Prognostic values and prospective pathway signaling of MicroRNA-182 in ovarian cancer: a study based on gene expression omnibus (GEO) and bioinformatics analysis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:31703725", "kind": "science-record", "revision": 7, "content_hash": "sha256:fd5e100216235b2fb6019af39d9712c22701a3b05677a136017519c4894dbb45", "created_at": "2026-11-01T15:40:51Z", "updated_at": "2026-11-01T22:00:59Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Prognostic values and prospective pathway signaling of MicroRNA-182 in ovarian cancer: a study based on gene expression omnibus (GEO) and bioinformatics analysis.", "doi": "10.1186/s13048-019-0580-7", "pmid": "31703725", "authors": ["Yaowei Li", "Li Li"], "journal": "Journal of ovarian research", "year": 2019, "abstract": "BACKGROUND: Ovarian carcinoma (OC) is a common cause of death among women with gynecological cancer. MicroRNAs (miRNAs) are believed to have vital roles in tumorigenesis of OC. Although miRNAs are broadly recognized in OC, the role of has-miR-182-5p (miR-182) in OC is still not fully elucidated.\nMETHODS: We evaluated the significance of miR-182 expression in OC by using analysis of a public dataset from the Gene Expression Omnibus (GEO) database and a literature review. Furthermore, we downloaded three mRNA datasets of OC and normal ovarian tissues (NOTs), GSE14407, GSE18520 and GSE36668, from GEO to identify differentially expressed genes (DEGs). Then the targeted genes of hsa-miR-182-5p (TG_miRNA-182-5p) were predicted using miRWALK3.0. Subsequently, we analyzed the gene overlaps integrated between DEGs in OC and predicted target genes of miR-182 by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. STRING and Cytoscape were used to construct a protein-protein interaction (PPI) network and the prognostic effects of the hub genes were analyzed.\nRESULTS: A common pattern of up-regulation for miR-182 in OC was found in our review of the literature. A total of 268 DEGs, both OC-related and miR-182-related, were identified, of which 133 genes were discovered from the PPI network. A number of DEGs were enriched in extracellular matrix organization, pathways in cancer, focal adhesion, and ECM-receptor interaction. Two hub genes, MCM3 and GINS2, were significantly associated with worse overall survival of patients with OC. Furthermore, we identified covert miR-182-related genes that might participate in OC by network analysis, such as DCN, AKT3, and TIMP2. The expressions of these genes were all down-regulated and negatively correlated with miR-182 in OC.\nCONCLUSIONS: Our study suggests that miR-182 is essential for the biological progression of OC.", "claims": [{"id": "claim-method", "statement": "The overall survival of patients with OC was analyzed using a Kaplan–Meier plot.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31703725", "locator": "sections[6] \"Survival analysis\" paragraph 2", "quote": "The overall survival of patients with OC was analyzed using a Kaplan–Meier plot."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31703725", "locator": "sections[25] \"notes\" paragraph 1", "quote": "The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:31703725"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE14407"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE14407"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:31703725", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1186/s13048-019-0580-7", "sha256": 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"licence_read": false}}, {"id": "asset:table:pmid:31703725:1", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:587225a4f2883024808024d9ecaf424a43301e66052348dfe6a563a7543de7c7", "bytes": 1558, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[15]/table-wrap[1]#Tab5", "version": null, "note": "Correlation between hsa-miR-182-5p (miR-182) and target genes in patients with ovarian cancer(n = 376)", "structure": {"pmcid": "PMC6839211", "section_index": 6, "section_title": "Survival analysis", "row_count": 22, "column_count": 5}, "table": {"label": "Table 5", "caption": "Correlation between hsa-miR-182-5p (miR-182) and target genes in patients with ovarian cancer(n = 376)", "rows": [["miRNA", "Target gene", "r", "p-value", "FDR"], ["hsa-miR-182-5p", "TPM1", "−0.143", "5.61E-03", "1.99E-02"], ["hsa-miR-182-5p", "COL1A1", "−0.154", "2.74E-03", "1.14E-02"], 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"identifier": "section[10]/table-wrap[1]#Tab2", "version": null, "note": "Overview of the 6 studies selected from literature", "structure": {"pmcid": "PMC6839211", "section_index": 10, "section_title": "Literature review of miR-182 expression profiles in OC versus NOTs", "row_count": 8, "column_count": 9}, "table": {"label": "Table 2", "caption": "Overview of the 6 studies selected from literature", "rows": [["Author", "Year", "Country", "Case", "Control", "Result", "Detection methods"], ["Source name", "n", "Source name", "n"], ["Wei Lu", "2016", "China", "OC", "12", "NOTs", "8", "Down-regulation", "qRT-PCR"], ["Barbara Marzec-Kotarska", "2016", "Poland", "EOC", "47", "NOTs", "26", "Up-regulation", "microRNA microarrays"], ["Lin Wang", "2014", "China", "OC", "41", "NOTs", "15", "Up-regulation", "Real-time PCR"], ["Bente Vilming Elgaaen", "2014", "Norway", "HGSC", "35", "NOTs", "9", "Up-regulation", "qRT-PCR"], ["Zhaojian Liu", "2012", "USA", "HG-PSC", "56", "FT", "21", 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"column_count": 4}, "table": {"label": "Table 3", "caption": "Top 10 hsa-miR-182-5p-related differentially expressed genes in ovarian cancer tissues compared with normal ovarian tissues according to data from Gene Expression Omnibus (GSE14407)", "rows": [["DEG", "logFC", "P.Value", "adj. P.Value"], ["up-regulated genes"], ["MECOM", "4.902333971", "9.81E-08", "1.99E-05"], ["TFAP2A", "4.745918435", "1.88E-08", "6.04E-06"], ["DEPDC1", "4.032946443", "5.66E-09", "3.03E-06"], ["AIF1L", "3.868411982", "3.79E-08", "9.54E-06"], ["BIRC5", "3.7736042", "8.61E-09", "3.89E-06"], ["BCL11A", "3.534029736", "1.11E-06", "1.13E-04"], ["L1CAM", "3.279255511", "1.66E-06", "1.49E-04"], ["HMMR", "3.262907245", "2.33E-06", "1.95E-04"], ["NCAPG", "3.229344903", "1.07E-08", "4.36E-06"], ["DTL", "3.208682435", "1.51E-10", "3.76E-07"], ["down-regulated genes"], ["PDE7B", "−5.031930277", "6.84E-09", "3.37E-06"], ["TCEAL7", "−4.928868798", "4.70E-09", "2.66E-06"], ["DCN", "−4.886965887", "1.16E-08", "4.61E-06"], ["GPM6A", "−4.799779935", "2.81E-08", "7.77E-06"], ["PGR", "−4.345763842", "2.85E-08", "7.83E-06"], ["PPM1E", "−4.270922539", "1.43E-08", "5.13E-06"], ["TMOD2", "−4.108261122", "5.21E-10", "7.49E-07"], ["NKX3–1", "−3.851343755", "1.34E-07", "2.48E-05"], ["TACC1", "−3.745549626", 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hsa-miR-182-5p-related differentially expressed genes in ovarian cancer", "rows": [["Term", "Description", "Count", "P-Value", "FDR"], ["Cellular components"], ["GO:0005634", "nucleus", "120", "4.67E-08", "6.23E-05"], ["GO:0005737", "cytoplasm", "108", "1.38E-05", "1.85E-02"], ["Molecular function"], ["GO:0005515", "protein binding", "173", "1.58E-07", "2.23E-04"], ["GO:0043565", "sequence-specific DNA binding", "23", "1.18E-05", "1.67E-02"], ["Biological processes"], ["GO:0030198", "extracellular matrix organization", "14", "1.00E-05", "1.70E-02"], ["KEGG pathway"], ["hsa05200", "Pathways in cancer", "22", "1.93E-06", "2.41E-03"], ["hsa04510", "Focal adhesion", "15", "8.20E-06", "1.02E-02"], ["hsa04512", "ECM-receptor interaction", "10", "1.38E-05", "1.72E-02"]], "row_count": 13, "column_count": 5, "numeric_cells": 32, "header_rows": 1, "locator": "section[13]/table-wrap[1]#Tab4"}}, {"id": "asset:table:pmid:31703725:6", "role": "table", "format": "jats-table", "media_type": 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