Long noncoding RNA LINC00518 acts as a competing endogenous RNA to promote the metastasis of malignant melanoma via miR-204-5p/AP1S2 axis.

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Although it has been reported that LINC00518 is dysregulated in melanoma, its exact role and molecular mechanism in melanoma remain unclear. RNA-seq analysis and qRT-PCR was used to detect the expression of LINC00518 in melanoma tissues. Melanoma cases from The Cancer Genome Atlas (TCGA), GEO#GSE15605 and GEO#GSE24469 were included in this study. 3D migration, transwell and scratch wound assay were used to explore the role of LINC00518 in melanoma cells. Bioinformatics, luciferase reporter assays, MS2-RIP assay, RNA pull-down assay and RNA-ChIP assay were used to demonstrate the mechanism of LINC00518 in melanoma. We found that LICN00518 was significantly upregulated in melanoma tissue, and high LICN00518 level was an independent risk factor for melanoma patients. LICN00518 promoted the invasion and migration of melanoma cells. LICN00518 exerted its role by decoying miR-204-5p to upregulate Adaptor Related Protein Complex 1 Sigma 2 Subunit (AP1S2) expression. We also demonstrated that LICN00518 promoted melanoma metastasis in vivo through pulmonary metastasis assay. This result elucidates a new mechanism for LICN00518 in the metastasis of melanoma. LICN00518 may serve as a survival indicator and potential therapeutic target in melanoma patients.", "claims": [{"id": "claim-method", "statement": "The data are expressed as the mean ± standard deviation and analyzed by SPSS13.0. The statistical significance of the data was evaluated by t test or one-way ANOVA. Spearman correlation analysis was performed by using MATLAB. Survival plots were drawn based on Kaplan−Meier analysis.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31712557", "locator": "sections[28] \"Statistical analysis\" paragraph 2", "quote": "The data are expressed as the mean ± standard deviation and analyzed by SPSS13.0. The statistical significance of the data was evaluated by t test or one-way ANOVA. Spearman correlation analysis was performed by using MATLAB. Survival plots were drawn based on Kaplan−Meier analysis."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Melanoma sample from The Cancer Genome Atlas (TCGA), GEO#GSE15605 and GEO#GSE24469 were also included in this study.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31712557", "locator": "sections[13] \"Patients and tissue samples\" paragraph 2", "quote": "Melanoma sample from The Cancer Genome Atlas (TCGA), GEO#GSE15605 and GEO#GSE24469 were also included in this study."}], "state": "complete"}, {"id": "claim-target-result", "statement": "High LINC00518 expression was an independent risk factor for melanoma patients, with a univariate hazard ratio of 2.538 and a multivariate hazard ratio of 2.295.", "kind": "quantitative", "target": {"value": 95.0, "unit": "per cent", "tolerance": null, "source": "LINC00518 expression (high vs. low)2.5381.115–5.7740.026Multivariate analysisHazard ratio95% CIP valueLINC00518 expression (high vs. low)2.2951.026–5.1320.043"}, "evidence": [{"asset": "source:pmid:31712557", "locator": "sections[4] \"Association between the expression of LINC00518 and prognosis in melanoma patients\" paragraph 2", "quote": "More importantly, univariate and multivariate Cox regression analyses further demonstrated that high LINC00518 expression is an independent risk factor for the melanoma patients (Table 2)."}, {"asset": "source:pmid:31712557", "locator": "sections[4] \"Association between the expression of LINC00518 and prognosis in melanoma patients\" paragraph 2", "quote": "LINC00518 expression (high vs. low)2.5381.115–5.7740.026Multivariate analysisHazard ratio95% CIP valueLINC00518 expression (high vs. low)2.2951.026–5.1320.043"}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "High LINC00518 expression was an independent risk factor for melanoma patients, with a univariate hazard ratio of 2.538 and a multivariate hazard ratio of 2.295.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:31712557"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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