HHIP overexpression inhibits the proliferation, migration and invasion of non-small cell lung cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:31765425", "kind": "science-record", "revision": 6, "content_hash": "sha256:1d1f26a9acc75865e28f1ff731a5c30c89cd9e7fa401d7347210439b1f12ed06", "created_at": "2026-11-01T15:40:52Z", "updated_at": "2026-11-01T21:22:39Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "HHIP overexpression inhibits the proliferation, migration and invasion of non-small cell lung cancer.", "doi": "10.1371/journal.pone.0225755", "pmid": "31765425", "authors": ["Jian-Guo Zhao", "Jian-Fang Wang", "Jiang-Feng Feng", "Xue-Ying Jin", "Wan-Li Ye"], "journal": "PloS one", "year": 2019, "abstract": "OBJECTIVE: The primary purpose of this study is to investigate the effect of hedgehog-interacting protein (HHIP) overexpression on the proliferation, migration and invasion of non-small cell lung cancer (NSCLC).\nMETHODS: Firstly, HHIP gene expression data of NSCLC tissues and normal tissues were obtained from GSE18842/GSE19804/GSE43458 databases of the Gene Expression Omnibus (GEO) database and then validated by TCGA NSCLC database in a cohort of 1027 cases of NSCLC patients and 108 cases of normal people. A chi-square test was used to analyze the relationship between HHIP expression and clinicopathological characteristics of NSCLC. The expression levels of HHIP in NSCLC cells were detected by quantitative-real time PCR. The function of HHIP was investigated by a series of in vitro assays. CCK-8, wounding healing, Transwell invasion assay were utilized to explore the mechanisms of HHIP.\nRESULTS: HHIP mRNA were significantly down-regulated in NSCLC in three GEO databases and TCGA database (P<0.05). This result was confirmed in NSCLC cell lines by qRT-PCR analysis, its expression in normal NSCLC cell line BEAS-2B was significantly higher than that in NSCLC cells. Chi-square test results showed that the low expression of HHIP was correlated with gender, cancer type, TNM stage and tumor size. Functional experimental results showed that over-expressing HHIP significantly decreased the ability of cell proliferation, migration and invasion in NSCLC cells (P<0.05).\nCONCLUSION: Overall, the above results indicated that HHIP could regulate proliferation, migration and invasion, and could be used as a judging criterion for identifying NSCLC classification and stage.", "claims": [{"id": "claim-method", "statement": "Paired samples t test was performed for 60 cases of lung adenocarcinoma tissues (2.709±0.323) and adjacent normal tissues (3.163±0.21) in GSE19804 and 44 cases of that (2.128±0.308 vs 2.936±0.188) in GSE18842, finding that the expression of HHIP mRNA in cancer tissues significantly down-regulated(Fig 1A and 1B) and the difference were statistically significant (P < 0.001). Independent samples t test was performed for GSE43458 data, finding that the expression of HHIP mRNA of cancer patients (2.865±0.158) was significantly lower than that of normal people (3.285±0.152) (Fig 1C).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31765425", "locator": "sections[13] \"The expression of HHIP significantly decreased in samples and cell strains of NSCLC patients\" paragraph 2", "quote": "Paired samples t test was performed for 60 cases of lung adenocarcinoma tissues (2.709±0.323) and adjacent normal tissues (3.163±0.21) in GSE19804 and 44 cases of that (2.128±0.308 vs 2.936±0.188) in GSE18842, finding that the expression of HHIP mRNA in cancer tissues significantly down-regulated(Fig 1A and 1B) and the difference were statistically significant (P < 0.001). Independent samples t test was performed for GSE43458 data, finding that the expression of HHIP mRNA of cancer patients (2.865±0.158) was significantly lower than that of normal people (3.285±0.152) (Fig 1C)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "All relevant data are within the manuscript and its Supporting Information files.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31765425", "locator": "sections[19] \"Data Availability\" paragraph 2", "quote": "All relevant data are within the manuscript and its Supporting Information files."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:31765425"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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