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pmid:31832020
Expression profile analysis identifies IER3 to predict overall survival and promote lymph node metastasis in tongue cancer.
Fang Xiao · Yinhua Dai · Yujiao Hu · Mengmeng Lu · Qun Dai
Cancer cell international · 2019 doi:10.1186/s12935-019-1028-2
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Experiment
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Analysis
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Complete file
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R software was used to identify the differentially expressed hallmark gene sets and individual genes between metastatic lymph node tissues and primary tongue cancer tissues, and the Kaplan-Meier method was used to evaluate the association with overall survival. The screening and validation of functional genes was performed using western blot, q-PCR, CCK-8, migration and invasion assays, and lymphangiogenesis was examined by using a tube formation assay.\nRESULTS: Thirteen common hallmark gene sets were found based on Gene Set Variation Analysis (GSVA) and then subjected to differential gene expression analysis, by which 76 deregulated genes were found. Gene coexpression network analysis and survival analysis further confirmed that IER3 was the key gene associated with the prognosis and lymph node metastasis of tongue cancer patients. Knockdown of IER3 with siRNA inhibited the proliferation, colony formation, migration and invasion of Tca-8113 cells in vitro and it also inhibited the secretion and expression of VEGF-C in these cells. The culture supernatant of Tca-8113 cells could promote lymphangiogenesis and migration of lymphatic endothelial cells, and knockdown of IER3 in Tca-8113 cells suppressed these processes.\nCONCLUSION: Our study demonstrated that IER3 plays important roles in lymphangiogenesis regulation and prognosis in tongue cancer and might be a potential therapeutic target.", "claims": [{"id": "claim-method", "statement": "Student’s t-test was used to calculate the P-value when comparing two groups. 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