Circular RNA circ-CMPK1 contributes to cell proliferation of non-small cell lung cancer by elevating cyclin D1 via sponging miR-302e.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:31863641", "kind": "science-record", "revision": 7, "content_hash": "sha256:a1df32c13c8707a7f6a094609cd2330b588a02d6345788a1c8bb715d62d3dd16", "created_at": "2026-11-01T15:40:53Z", "updated_at": "2026-11-01T21:22:45Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Circular RNA circ-CMPK1 contributes to cell proliferation of non-small cell lung cancer by elevating cyclin D1 via sponging miR-302e.", "doi": "10.1002/mgg3.999", "pmid": "31863641", "authors": ["Dong Cui", "Runlin Qian", "Yin Li"], "journal": "Molecular genetics & genomic medicine", "year": 2020, "abstract": "BACKGROUND: It is well recognized that competing endogenous RNA (ceRNA) regulatory network is linked to the development and progression of cancer, including non-small cell lung cancer (NSCLC). Herein, we aimed to explore the functional role of circ-CMPK1/miR-302e/cyclin D1 ceRNA signaling in NSCLC.\nMETHODS: GEO database (GSE102287) was utilized to screen differentially expressed miRNAs in NSCLC. Quantitative reverse transcription PCR (qRT-PCR) and western blotting assays were used to determine gene expression. Cell proliferation analysis was performed with Cell Counting Kit-8 (CCK-8) and cell cycle assays. Luciferase reporter and RNA pull-down assays were conducted to identify the interaction among circ-CMPK1, miR-302e, and cyclin D1. Xenograft tumor model was established to evaluate the role of circ-CMPK1/miR-302e/cyclin D1 axis in vivo.\nRESULTS: miR-302e expression was significantly down-regulated in NSCLC cell lines and tissues and its decrease was closely associated with aggressive clinicopathological features and unfavorable outcome. Overexpression and knockdown of miR-302e obviously retarded and enhanced the growth of NSCLC, respectively. Furthermore, we found that miR-302 was sponged by circular RNA CMPK1 (circ-CMPK1, hsa_circ_0012384), which was remarkably up-regulated in NSCLC and predicted poor prognosis. Circ-CMPK1 was capable to promote NSCLC cells proliferation by increasing the expression of cyclin D1 via inhibiting miR-302 activity. Moreover the miR-302e-mediated tumor inhibition could be effectively counteracted by ectopic expression of circ-CMPK1 or cyclin D1 both in vitro and in vivo.\nCONCLUSION: Our data demonstrate for the first time that circ-CMPK1/miR-302e/cyclin D1 signaling plays an essential regulatory role in NSCLC and targeting this axis may be an efficacious avenue for treatment of NSCLC patients.", "claims": [{"id": "claim-data-access", "statement": "To identify NSCLC‐related miRNAs, we first analyzed the GEO database (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE102287) using the GEO2R method (https://www.ncbi.nlm.nih.gov/geo/geo2r/?acc=GSE102287).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31863641", "locator": "sections[14] \"miR‐302e is significantly decreased in NSCLC cell lines and tissues\" paragraph 2", "quote": "To identify NSCLC‐related miRNAs, we first analyzed the GEO database (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE102287) using the GEO2R method (https://www.ncbi.nlm.nih.gov/geo/geo2r/?acc=GSE102287)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:31863641"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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Quantitative reverse transcription PCR (qRT-PCR) and western blotting assays were u"], "order_basis": "paper_text_order:488", "id": "assay:1"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["tive reverse transcription PCR (qRT-PCR) and western blotting assays were used to determine gene expressio"], "order_basis": "paper_text_order:541", "id": "assay:2"}, {"role": "data_generation", "definition_id": "apoptosis_assay", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["ysis was performed with Cell Counting Kit-8 (CCK-8) and cell cycle assays. Luciferase reporter"], "order_basis": "paper_text_order:673", "id": "assay:3"}, {"role": "data_generation", "definition_id": "animal_model", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["e role of circ-CMPK1/miR-302e/cyclin D1 axis in vivo. RESULTS: miR-302e expression was significan", "of circ-CMPK1 or cyclin D1 both in vitro and in vivo. CONCLUSION: Our data demonstrate for the fi", "/c nude mice (n = 5 in each group). All nude mice were male with 4–6‐week‐old and purchased from th", "ry 7 days. 35 days after the injection, nude mice were all sacrificed and the tumor tissues were ob"], "order_basis": "paper_text_order:931", "id": "assay:4"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["and unfavorable outcome. Overexpression and knockdown of miR-302e obviously retarded and enhanced", "istics in 80 NSCLC patients Cell culture and transfection All NSCLC cell lines including A549, SPC‐A1,", "640 medium with 10% fetal bovine serum. Cell transfection was carried out by using Lipofectamine 2000", "sh xenograft tumor model, 5 × 106 A549 cells transfected with the indicated oligonucleotides or vecto"], "order_basis": "paper_text_order:1164", "id": "assay:5"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["transfection All NSCLC cell lines including A549, SPC‐A1, HCC827, 95‐D, H1299, H460 and a nor", "To establish xenograft tumor model, 5 × 106 A549 cells transfected with the indicated oligonu"], "order_basis": "paper_text_order:2632", "id": "assay:6"}, {"role": "analysis", "definition_id": "correlation_analysis", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["pearson_r"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 0.8, "evidence": ["Pearson's correlation"], "order_basis": "consumes_earlier_output", "id": "assay:7"}]}