Quantitative Proteomics Reveal an Altered Pattern of Protein Expression in Brain Tissue from Mice Lacking GPR37 and GPR37L1.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:31903766", "kind": "science-record", "revision": 4, "content_hash": "sha256:357b1c488b65a2b0c5367594e153e7edd801138b695d6fbd9b8e14962990f85c", "created_at": "2026-11-01T17:33:18Z", "updated_at": "2026-11-01T22:01:10Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Quantitative Proteomics Reveal an Altered Pattern of Protein Expression in Brain Tissue from Mice Lacking GPR37 and GPR37L1.", "doi": "10.1021/acs.jproteome.9b00622", "pmid": "31903766", "authors": ["TrangKimberly Thu Nguyen", "Eric B Dammer", "Sharon A Owino", "Michelle M Giddens", "Nora S Madaras", "Duc M Duong", "Nicholas T Seyfried", "Randy A Hall"], "journal": "Journal of proteome research", "year": 2020, "abstract": "GPR37 and GPR37L1 are glia-enriched G protein-coupled receptors that have been implicated in several neurological and neurodegenerative diseases. To gain insight into the potential molecular mechanisms by which GPR37 and GPR37L1 regulate cellular physiology, proteomic analyses of whole mouse brain tissue from wild-type (WT) versus GPR37/GPR37L1 double knockout (DKO) mice were performed in order to identify proteins regulated by the absence versus presence of these receptors (data are available via ProteomeXchange with identifier PXD015202). These analyses revealed a number of proteins that were significantly increased or decreased by the absence of GPR37 and GPR37L1. One of the most decreased proteins in the DKO versus WT brain tissue was S100A5, a calcium-binding protein, and the reduction of S100A5 expression in KO brain tissue was validated via Western blot. Coexpression of S100A5 with either GPR37 or GPR37L1 in HEK293T cells did not result in any change in S100A5 expression but did robustly increase secretion of S100A5. To dissect the mechanism by which S100A5 secretion was enhanced, cells coexpressing S100A5 with the receptors were treated with different pharmacological reagents. These studies revealed that calcium is essential for the secretion of S100A5 downstream of GPR37 and GPR37L1 signaling, as treatment with BAPTA-AM, an intracellular Ca2+ chelator, reduced S100A5 secretion from transfected HEK293T cells. Collectively, these findings provide a panoramic view of proteomic changes resulting from loss of GPR37 and GPR37L1 and also impart mechanistic insight into the regulation of S100A5 by these receptors, thereby shedding light on the functions of GPR37 and GPR37L1 in brain tissue.", "claims": [{"id": "claim-method", "statement": "Significant differentially expressed proteins were then determined by additional filtering for t-test p values less than 0.05.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31903766", "locator": "sections[7] \"Data processing and determination of differential protein abundance in GPR37/GPR37L1 double knockout vs. wild-type\" paragraph 2", "quote": "Significant differentially expressed proteins were then determined by additional filtering for t-test p values less than 0.05."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE24 partner repository with the dataset identifier PXD015202.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31903766", "locator": "sections[6] \"Database search and label-free quantification\" paragraph 2", "quote": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE24 partner repository with the dataset identifier PXD015202."}], "state": "complete"}, {"id": "claim-target-result", "statement": "These analyses revealed a number of proteins that were significantly increased or decreased by the absence of GPR37 and GPR37L1.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:31903766", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "These analyses revealed a number of proteins that were significantly increased or decreased by the absence of GPR37 and GPR37L1."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "These analyses revealed a number of proteins that were significantly increased or decreased by the absence of GPR37 and GPR37L1.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:31903766"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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All mice were maintained on a C57BL/6J background and hous", "hole brain lysates from adult (3 months old) mice were prepared in urea lysis buffer as previously"], "order_basis": "paper_text_order:367", "id": "assay:1"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["ression in KO brain tissue was validated via Western blot. Coexpression of S100A5 with either GPR37 or", "databases)Supplemental Figure S5. Uncropped Western blots of Figure 2A (S100A5 protein and mRNA levels", "mouse brainSupplemental Figure S6. Uncropped Western blots of Figure 3C (Co-expression of S100A5 with e", "293T cells)Supplemental Figure S7. Uncropped Western blots of Figure 4A (Receptor regulation of S100A5"], "order_basis": "paper_text_order:940", "id": "assay:2"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["Ca2+ chelator, reduced S100A5 secretion from transfected HEK293T cells. Collectively, these findings", "les were excluded. Cell culture and cellular transfection Human embryonic kidney cells (HEK293T cells)", "re plated at 40% confluence the day prior to transfection. For standard transfections, 2 μg of each DN", "ng to the manufacturer’s protocol. 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