Lowered levels of microRNA-129 and potential signaling pathways in papillary thyroid carcinoma: a determination of microRNA sequencing in 507 patients and bioinformatics analysis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:31966595", "kind": "science-record", "revision": 4, "content_hash": "sha256:c5f9b2ff155435d48cb6353a7c1bf90ff40099df52ff6ec6c07a11e21f413e35", "created_at": "2026-11-01T17:33:19Z", "updated_at": "2026-11-01T22:01:13Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "partial", "title": "Lowered levels of microRNA-129 and potential signaling pathways in papillary thyroid carcinoma: a determination of microRNA sequencing in 507 patients and bioinformatics analysis.", "doi": null, "pmid": "31966595", "authors": ["Liang Liang", "Yihuan Luo", "Xia Yang", "Rui Zhang", "Hanlin Wang", "Hong Yang", "Yun He", "Gang Chen", "Wei Ma", "Junqiang Chen"], "journal": "International journal of clinical and experimental pathology", "year": 2017, "abstract": "Papillary thyroid carcinoma (PTC) is one of the most common endocrine system malignancies. However, the mechanism of tumor development is unclear. microRNA-129-5p is a microRNA that plays an important role in the development of tumors. The main purpose of our article is to find the potential target genes of microRNA-129 and their pathways based on gene array, sequencing and bioinformatics studies. We obtained microRNA-129 expression and clinical associations in the TCGA database. In addition, we found a microRNA-129-related chip GSE19933, which is overexpressing microR-129-5p in thyroid cancer cell lines. The down-regulated gene is considered to be a potential target gene for microRNA-129. The target genes were predicted through 12 online tools. We performed Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of all down-regulated and predicted target genes. Furthermore, protein-protein interactions (PPI) were also analyzed for all potential genes. Finally, with intersecting down-regulated genes by overexpressed microRNA-129 and predicted target genes, the 889 genes are mainly enriched in the calcium signaling pathway, cGMP-PKG signaling pathway, ErbB signaling pathway and Proteoglycans in cancer, etc. The role of ten hub genes is particularly prominent in PPI analysis. These genes are differentially expressed in the thyroid by immunohistochemistry. We confirmed that microRNA-129 may play a major role in PTC through the above pathways, but more experiments are still needed to prove our results.", "claims": [{"id": "claim-method", "statement": "We performed Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of all down-regulated and predicted target genes.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31966595", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "We performed Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of all down-regulated and predicted target genes."}], "state": "complete"}, {"id": "claim-data-access", "statement": "We obtained microRNA-129 expression and clinical associations in the TCGA database.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31966595", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "We obtained microRNA-129 expression and clinical associations in the TCGA database."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The 889 genes intersecting down-regulated genes by overexpressed microRNA-129 and predicted target genes were mainly enriched in the calcium signaling pathway, cGMP-PKG signaling pathway, ErbB signaling pathway and Proteoglycans in cancer.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:31966595", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Finally, with intersecting down-regulated genes by overexpressed microRNA-129 and predicted target genes, the 889 genes are mainly enriched in the calcium signaling pathway, cGMP-PKG signaling pathway, ErbB signaling pathway and Proteoglycans in cancer, etc."}], "state": "complete"}], "gaps": ["doi"]}, "experiment": {"state": "complete", "objective": "The 889 genes intersecting down-regulated genes by overexpressed microRNA-129 and predicted target genes were mainly enriched in the calcium signaling pathway, cGMP-PKG signaling pathway, ErbB signaling pathway and Proteoglycans in cancer.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:31966595"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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