Hat1-Dependent Lysine Acetylation Targets Diverse Cellular Functions.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:32081014", "kind": "science-record", "revision": 3, "content_hash": "sha256:508dd10ec36d142139f0a1769f1af2496a7c0dea97d59614f60f0f676943df9b", "created_at": "2026-11-01T17:33:24Z", "updated_at": "2026-11-01T21:22:55Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Hat1-Dependent Lysine Acetylation Targets Diverse Cellular Functions.", "doi": "10.1021/acs.jproteome.9b00843", "pmid": "32081014", "authors": ["Paula A Agudelo Garcia", "Prabakaran Nagarajan", "Mark R Parthun"], "journal": "Journal of proteome research", "year": 2020, "abstract": "Lysine acetylation has emerged as one of the most important post-translational modifications, regulating different biological processes. However, its regulation by lysine acetyltransferases is still unclear in most cases. Hat1 is a lysine acetyltransferase originally identified based on its ability to acetylate histones. Using an unbiased proteomics approach, we have determined how loss of Hat1 affects the mammalian acetylome. Hat1+/+ and Hat1-/- mouse embryonic fibroblast cell lines were grown in both glucose- and galactose-containing media, as Hat1 is required for growth on galactose, and Hat1-/- cells exhibit defects in mitochondrial function. Following trypsin digestion of whole cell extracts, acetylated peptides were enriched by acetyllysine affinity purification, and acetylated peptides were identified and analyzed by label-free quantitation. Comparison of the acetylome from Hat1+/+ cells grown on galactose and glucose demonstrated that there are large carbon source-dependent changes in the mammalian acetylome where the acetylation of enzymes involved in glycolysis were the most affected. Comparisons of the acetylomes from Hat1+/+ and Hat1-/- cells identified 65 proteins whose acetylation decreased by at least 2.5-fold in cells lacking Hat1. In Hat1-/- cells, acetylation of the autoregulatory loop of CBP (CREB-binding protein) was the most highly affected, decreasing by up to 20-fold. In addition to the proteins involved in chromatin structure, Hat1-dependent acetylation was also found in a number of transcriptional regulators, including p53 and mitochondrial proteins. Hat1 mitochondrial localization suggests that it may be directly involved in the acetylation of mitochondrial proteins. Data are available via ProteomeXchange with identifier PXD017362.", "claims": [{"id": "claim-method", "statement": "The resulting peptides were analyzed by LC-MS/MS, and the abundance of the acetylated peptides in each sample was determined using label free-quantitation. The log2 fold change was calculated using the relative fold change between the integrated peak area of the experimental conditions (Hat1+/+ galactose, Hat1−/− glucose or Hat1−/− galactose) and the control (Hat1+/+ glucose). Values greater than ±2.5 were used for subsequent analysis.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32081014", "locator": "sections[9] \"Mammalian Acetylome Altered by a Shift from Glucose to Galactose\" paragraph 3", "quote": "The resulting peptides were analyzed by LC-MS/MS, and the abundance of the acetylated peptides in each sample was determined using label free-quantitation."}, {"asset": "source:pmid:32081014", "locator": "sections[7] \"PTM Scan Lysine Acetylation\" paragraph 2", "quote": "The log2 fold change was calculated using the relative fold change between the integrated peak area of the experimental conditions (Hat1+/+ galactose, Hat1−/− glucose or Hat1−/− galactose) and the control (Hat1+/+ glucose)."}, {"asset": "source:pmid:32081014", "locator": "sections[7] \"PTM Scan Lysine Acetylation\" paragraph 2", "quote": "Values greater than ±2.5 were used for subsequent analysis."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data are available via ProteomeXchange with identifier PXD017362.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32081014", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Data are available via ProteomeXchange with identifier PXD017362."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Comparisons of the acetylomes from Hat1+/+ and Hat1−/− cells identified 65 proteins whose acetylation decreased by at least 2.5 fold in cells lacking Hat1.", "kind": "quantitative", "target": {"value": 2.5, "unit": "fold change", "tolerance": null, "source": "Comparisons of the acetylomes from Hat1+/+ and Hat1−/− cells identified 65 proteins whose acetylation decreased by at least 2.5 fold in cells lacking Hat1."}, "evidence": [{"asset": "source:pmid:32081014", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Comparisons of the acetylomes from Hat1+/+ and Hat1−/− cells identified 65 proteins whose acetylation decreased by at least 2.5 fold in cells lacking Hat1."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Comparisons of the acetylomes from Hat1+/+ and Hat1−/− cells identified 65 proteins whose acetylation decreased by at least 2.5 fold in cells lacking Hat1.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:32081014"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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