CDK1 and CDC20 overexpression in patients with colorectal cancer are associated with poor prognosis: evidence from integrated bioinformatics analysis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:32127012", "kind": "science-record", "revision": 8, "content_hash": "sha256:764e608ec8aeaadf9738365dc83ce4d94c9b6f10dc93fd44edbcf6cfbc170a40", "created_at": "2026-11-01T15:40:57Z", "updated_at": "2026-11-01T22:44:37Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "CDK1 and CDC20 overexpression in patients with colorectal cancer are associated with poor prognosis: evidence from integrated bioinformatics analysis.", "doi": "10.1186/s12957-020-01817-8", "pmid": "32127012", "authors": ["Jianxin Li", "Yinchun Wang", "Xin Wang", "Qingqiang Yang"], "journal": "World journal of surgical oncology", "year": 2020, "abstract": "BACKGROUND: Colorectal cancer (CRC) is one of the most common malignancies of the digestive system, which causes severe financial burden worldwide. However, the specific mechanisms involved in CRC are still unclear.\nMETHODS: To identify the significant genes and pathways involved in the initiation and progression of CRC, the microarray dataset GSE126092 was downloaded from Gene Expression Omnibus (GEO) database, and then, the data was analyzed to identify differentially expressed genes (DEGs). Subsequently, the Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed on these DEGs using the DAVID database, and the protein-protein interaction (PPI) network was constructed using the STRING database and analyzed using the Cytoscape software. Finally, hub genes were screened, and the survival analysis was performed on these hub genes using the Kaplan-Meier curves in the cBioPortal database.\nRESULTS: In total, 937 DEGs were obtained, including 316 upregulated genes and 621 downregulated genes. GO analysis revealed that the DEGs were mostly enriched in terms of nuclear division, organelle fission, cell division, and cell cycle process. KEGG pathway analysis showed that the DEGs were mostly enriched in cell cycle, oocyte meiosis, cytokine-cytokine receptor interaction, and cGMP-PKG signaling pathway. The PPI network comprised 608 nodes and 3100 edges, and 4 significant modules and 10 hub genes with the highest degree were identified using the Cytoscape software. Finally, survival analysis showed that overexpression of CDK1 and CDC20 in patients with CRC were statistically associated with worse overall survival.\nCONCLUSIONS: This bioinformatics analysis revealed that CDK1 and CDC20 might be candidate targets for diagnosis and treatment of CRC, which provided valuable clues for CRC.", "claims": [{"id": "claim-method", "statement": "In order to identify the potential prognostic role of these hub genes, the survival analysis of hub genes was performed using the Kaplan-Meier curves in the cBioPortal database (http://www.cbioportal.org) [16], and log rank test P value < 0.05 being the threshold of statistical significance.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32127012", "locator": "sections[7] \"Survival analysis and validation of gene expression\" paragraph 2", "quote": "In order to identify the potential prognostic role of these hub genes, the survival analysis of hub genes was performed using the Kaplan-Meier curves in the cBioPortal database (http://www.cbioportal.org) [16], and log rank test P value < 0.05 being the threshold of statistical significance."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets generated and/or analyzed during the current study are available in the Gene Expression Omnibus (GEO) repository (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE126092).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32127012", "locator": "sections[20] \"notes\" paragraph 1", "quote": "The datasets generated and/or analyzed during the current study are available in the Gene Expression Omnibus (GEO) repository (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE126092)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:32127012"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE126092"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE126092"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:32127012", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1186/s12957-020-01817-8", "sha256": "sha256:b5632f780639e18838ad6ab49654a9e5575f90114129fce109173e0db8e243b2", "bytes": 32670, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1186/s12957-020-01817-8", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC7055103", "source": "europe_pmc_fulltext", "coverage": "whole_body_without_references", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE126092", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE126092", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE126092", "version": null, "note": "The datasets generated and/or analyzed during the current study are available in the Gene Expression Omnibus (GEO) repository (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE126092).", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The datasets generated and/or analyzed during the current study are available in the Gene Expression Omnibus (GEO) repository (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE126092).", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM3591506", "GSM3591507", "GSM3591508", "GSM3591509", "GSM3591510", "GSM3591511", "GSM3591512", "GSM3591513", "GSM3591514", "GSM3591515", "GSM3591516", "GSM3591517", "GSM3591518", "GSM3591519", "GSM3591520", "GSM3591521", "GSM3591522", "GSM3591523", "GSM3591524", "GSM3591525"], "sample_count": 20, "group_sizes": null, "group_sizes_basis": null, "platform": "21047", "taxon": "Homo sapiens", "title": "Hsa_circ_101555 functions as a competing endogenous RNA of miR-597-5p to promote colorectal cancer progression [lncRNA/mRNAs]", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "asset:table:pmid:32127012:1", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:c19b6ad2ac01643d401776c09f5a7c928d4c06a56e60ead9f3a749a482d056e2", "bytes": 1990, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[11]/table-wrap[1]#Tab2", "version": null, "note": "Functional roles of 10 hub genes", "structure": {"pmcid": "PMC7055103", "section_index": 6, "section_title": "Integration of protein-protein interaction network and module analysis", "row_count": 11, "column_count": 4}, "table": {"label": "Table 2", "caption": "Functional roles of 10 hub genes", "rows": [["No.", "Gene symbol", "Full name", "Function"], ["1", "CDK1", "Cyclin-dependent kinase 1", "Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset"], ["2", "CDC20", "Cell division cycle protein 20 homolog", "Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome and may confer substrate specificity upon the complex"], ["3", "AURKA", "Aurora kinase A", "Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression."], ["4", "PLK1", "Polo-like kinase 1", "Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle"], ["5", "AURKB", "Aurora kinase B", "Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis"], ["6", "CDC6", "Cell division control protein 6 homolog", "Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated"], ["7", "KIF11", "Kinesin family member 11", "Motor protein required for establishing a bipolar spindle during mitosis. Required in nonmitotic cells for transport of secretory proteins from the Golgi complex to the cell surface"], ["8", "CCNA2", "Cyclin A2", "Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle"], ["9", "CENPE", "Centromere-associated protein E", "Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation, and spindle assembly checkpoint activation"], ["10", "MKI67", "Marker of proliferation Ki-67", "Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly"]], "row_count": 11, "column_count": 4, "numeric_cells": 19, "header_rows": 1, "locator": "section[11]/table-wrap[1]#Tab2"}}, {"id": "asset:table:pmid:32127012:2", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:e5cd0d99b18ed07a7291db4f7a2b2ec5b4bf8906ba7d53bdeb08a1c109eaa038", "bytes": 3109, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[10]/table-wrap[1]#Tab1", "version": null, "note": "Gene Ontology and KEGG pathway analysis of DEGs in colorectal cancer", "structure": {"pmcid": "PMC7055103", "section_index": 10, "section_title": "GO and KEGG enrichment analyses of DEGs", "row_count": 41, "column_count": 5}, "table": {"label": "Table 1", "caption": "Gene Ontology and KEGG pathway analysis of DEGs in colorectal cancer", "rows": [["Expression", "Category", "Term", "Count", "P value"], ["Upregulated", "GOTERM_BP_FAT", "GO:0000280~nuclear division", "40", "1.34E−18"], ["GOTERM_BP_FAT", "GO:0048285~organelle fission", "40", "1.23E−17"], ["GOTERM_BP_FAT", "GO:0051301~cell division", "37", "2.68E−16"], ["GOTERM_BP_FAT", "GO:0022402~cell cycle process", "56", "4.22E−16"], ["GOTERM_BP_FAT", "GO:0007049~cell cycle", "61", "3.43E−15"], ["GOTERM_CC_FAT", "GO:0005694~chromosome", "41", "1.95E−11"], 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"identifier": "section[11]/table-wrap[1]#Tab2", "version": null, "note": "Functional roles of 10 hub genes", "structure": {"pmcid": "PMC7055103", "section_index": 11, "section_title": "Integration of protein-protein interaction network and module analysis", "row_count": 11, "column_count": 4}, "table": {"label": "Table 2", "caption": "Functional roles of 10 hub genes", "rows": [["No.", "Gene symbol", "Full name", "Function"], ["1", "CDK1", "Cyclin-dependent kinase 1", "Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset"], ["2", "CDC20", "Cell division cycle protein 20 homolog", "Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome and may confer substrate specificity upon the complex"], ["3", "AURKA", "Aurora kinase A", "Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression."], ["4", "PLK1", "Polo-like kinase 1", "Serine/threonine-protein 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Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated"], ["7", "KIF11", "Kinesin family member 11", "Motor protein required for establishing a bipolar spindle during mitosis. Required in nonmitotic cells for transport of secretory proteins from the Golgi complex to the cell surface"], ["8", "CCNA2", "Cyclin A2", "Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle"], ["9", "CENPE", "Centromere-associated protein E", "Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation, and spindle assembly checkpoint activation"], ["10", "MKI67", "Marker of proliferation Ki-67", "Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly"]], "row_count": 11, "column_count": 4, "numeric_cells": 19, "header_rows": 1, "locator": "section[11]/table-wrap[1]#Tab2"}}], "gaps": ["asset:data:geo:GSE126092 not downloaded", "asset:data:geo:GSE126092 licence not read"]}, "analysis": {"state": "complete", "methods": [{"id": "method-1", "family": "survival_analysis", "specific": "kaplan_meier", "label": "Survival analysis", "dsl_operations": ["survival_km"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["time_to_event_with_censoring"], "evidence": {"score": 1.0, "band": "verbatim", "quote": "In order to identify the potential prognostic role of these hub genes, the survival analysis of hub genes was performed using the Kaplan-Meier curves in the cBioPortal database (http://www.cbioportal.org) [16], and log rank test P value < 0.05 being the threshold of statistical significance."}}, {"id": "method-2", "family": "survival_analysis", "specific": "log_rank_test", "label": "Survival analysis", "dsl_operations": ["logrank_test"], "missing_operations": [], "expressible": true, "parameters": {"p_value": "[16], and log rank test P value < 0"}, "data_shapes": ["time_to_event_with_censoring"], "evidence": {"score": 0.8, "band": "high", "quote": "log rank"}}, {"id": "method-3", "family": "network_analysis", "specific": "ppi_network", "label": "Network / pathway analysis", "dsl_operations": ["build_graph", "centrality_scores", "pathway_enrich"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["edge_list"], "evidence": {"score": 0.7, "band": "medium", "quote": "protein-protein interaction"}}], "targets": [], "gaps": []}, "capability": {"state": "complete", "requirements": [{"capability_id": "capability:computational_analysis", "label": "Computational analysis platform", "assay_families": ["meta_analysis", "machine_learning", "survival_analysis", "statistical_regression", "pca", "molecular_docking", "network_analysis", "differential_expression", "gwas"], "availability_state": "owned", "tier_unlocked": "R1", "candidate": "Automated Assays Laravel application and simulated adapters"}], "gaps": []}, "readiness": {"state": "complete", "tier": "R1", "blocking_axis": "none", "blocked_on": "nothing:replicable_now", "axes": {"method": {"tier": "R1", "expressible": true, "required_operations": ["survival_km"], "missing_operations": [], "basis": "The paper names the method it used and the corpus's method extractor maps it onto the DSL catalogue: kaplan_meier."}, "capability": {"tier": "R1", "capability_id": "capability:computational_analysis", "availability_state": "owned", "basis": "The computational analysis is owned. 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