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pmid:32154175
miR-1323 Promotes Cell Migration in Lung Adenocarcinoma by Targeting Cbl-b and Is an Early Prognostic Biomarker.
Huan Zhao · Chunlei Zheng · Yizhe Wang · Kezuo Hou · Xianghong Yang · Yang Cheng · Xiaofang Che · Shilin Xie · Shuo Wang · Tieqiong Zhang · Jian Kang · Yunpeng Liu · Dianzhu Pan · Xiujuan Qu · Xuejun Hu · Yibo Fan
Frontiers in oncology · 2020 doi:10.3389/fonc.2020.00181
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Complete file
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However, identification of specific microRNAs in NSCLC as potential therapeutic targets is controversial. We aim to determine the clinical significance of miR-1323 in the prognosis of patients with lung cancer and the potential mechanism. Patients and methods: A bioinformatics approach was used to screen the importance microRNA in NSCLC through the online GEO database (GSE42425). The relationship between expression level of miR-1323 and overall survival of lung cancer patients was analyzed. Additionally, an independent corhort including 53 NSCLC cases that underwent resection validated the connection between miR-1323 and LUAD patients' overall survival. Next, the function of miR-1323 was studied in vitro by transient transfection. A more in-depth mechanism was studied through luciferase reporter gene experiments. Results: High miR-1323 expression correlated with poor survival in NSCLC patients (P = 0.011), and in lung adenocarcinoma (LUAD) patients (P = 0.015) based on GEO database (GSE42425). In the independent cohort based on our hospital, high miR-1323 expression was associated with LUAD patients (P = 0.025). Moreover, transfection with mimics of miR-1323 showed an increased migratory capacity in LUAD A549 and HCC827 cells. In addition, E3 ubiquitin-protein ligase Casitas B-lineage Lymphoma-b (Cbl-b) was found to be the target genes of miR-1323 and significantly down regulated after mimics of miR-1323 transfection, and high Cbl-b expression predicted better prognosis in NSCLC and LUAD (P = 0.00072 and P = 0.02, respectively). Conclusion: The miR-1323 promoted LUAD migration through inhibiting Cbl-b expression. High miR-1323 expression predicted poor prognosis in LUAD patients.", "claims": [{"id": "claim-method", "statement": "Kaplan-Meier analysis were performed to do the survival analysis.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32154175", "locator": "sections[4] \"Bioinformatics Analysis\" paragraph 4", "quote": "Kaplan-Meier analysis were performed to do the survival analysis."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Publicly available datasets were analyzed in this study. This data can be found here: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE42425. The datasets generated for this study are available on request to the corresponding author.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32154175", "locator": "sections[21] \"Data Availability Statement\" paragraph 2", "quote": "Publicly available datasets were analyzed in this study. 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