Screening and identification of critical biomarkers in erectile dysfunction: evidence from bioinformatic analysis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:32161689", "kind": "science-record", "revision": 7, "content_hash": "sha256:708a45616255459c0b3a4a24310b1d277af2a29df96da734c12d54046569169a", "created_at": "2026-11-01T15:40:57Z", "updated_at": "2026-11-01T22:01:22Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Screening and identification of critical biomarkers in erectile dysfunction: evidence from bioinformatic analysis.", "doi": "10.7717/peerj.8653", "pmid": "32161689", "authors": ["Jialiang Hui", "Ruiyu Liu", "Haibo Zhang", "Shuhua He", "Anyang Wei"], "journal": "PeerJ", "year": 2020, "abstract": "PURPOSE: Erectile dysfunction (ED) is one of the most common male-disease globally. Despite efforts to explain its pathogenesis, the molecular mechanisms of ED are still not well understood.\nMETHODS: The microarray dataset GSE10804 was downloaded from the Gene Expression Omnibus (GEO) to find candidate genes in ED progression. After differentially expressed genes (DEGs) were identified, functional enrichment analysis was performed. In addition, a protein-protein interaction network (PPI) was established and module analysis was performed through the STRING and Cytoscape.\nRESULTS AND CONCLUSIONS: A total of 618 DEGs were identified in all, containing 430 downregulated genes and 188 upregulated genes. The enriched functions and pathways of the DEGs include transcription from RNA polymerase II promoter, cell adhesion, calcium ion binding, receptor binding, Akt signaling pathway, receptor interaction, protein digestion, and absorption. We picked out twenty-five hub genes, with biological process (BP) analyses revealing that the genes were principally associated with cellular responses to amino acid stimuli, extracellular matrix structural constituent, collagen trimer, protein digestion and absorption, ECM-receptor interaction and PI3K-Akt signaling pathway. To sum up, DEGs and hub genes distinguished in this study not only help us understand the molecular mechanisms behind the carcinogenesis and progression of ED, but also play a part in the diagnosis and treatment of ED by providing candidate targets.", "claims": [{"id": "claim-method", "statement": "The DEGs in the ED and non-ED specimen were picked out from GEO2R (http://www.ncbi.nlm.nih.gov/geo/geo2r), which is a platform for examing DEGs across experimental conditions by comparing multiple datasets in a GEO series.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32161689", "locator": "sections[4] \"Identification of DEGs\" paragraph 2", "quote": "The DEGs in the ED and non-ED specimen were picked out from GEO2R (http://www.ncbi.nlm.nih.gov/geo/geo2r), which is a platform for examing DEGs across experimental conditions by comparing multiple datasets in a GEO series."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The following information was supplied regarding data availability:Raw data is available at NCBI GEO: GSE10804.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32161689", "locator": "sections[15] \"Additional Information and Declarations\" paragraph 4", "quote": "The following information was supplied regarding data availability:Raw data is available at NCBI GEO: GSE10804."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:32161689"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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