Hsa_circ_103973 Acts as a Sponge of miR-335 to Promote Cervical Cancer Progression.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:32184614", "kind": "science-record", "revision": 9, "content_hash": "sha256:4f284ea60976dc612647305dba52290bf5d8df7f3cedd02fb6e110c51cffa5d8", "created_at": "2026-11-01T15:40:58Z", "updated_at": "2026-11-01T22:44:40Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Hsa_circ_103973 Acts as a Sponge of miR-335 to Promote Cervical Cancer Progression.", "doi": "10.2147/OTT.S215736", "pmid": "32184614", "authors": ["Yingping Zhu", "Xuelu Jiang", "Shuo Zhang", "Lingcong Wang", "Qun Zhou", "Jun Jiang"], "journal": "OncoTargets and therapy", "year": 2020, "abstract": "BACKGROUND: Cervical cancer (CC) ranks as the second most common malignancy in women, accounting for more two 2 million deaths every year in the world. Recently, circular RNAs (circRNAs) have been reported to regulate the progression of multiple human tumors; however, whether it involves in CC remains largely elusive.\nMATERIALS AND METHODS: Two GEO circRNA expression profiles (GSE102686, GSE113696) were downloaded to analyze the differentially expressed circRNAs using bioinformatics methods. Expression of circ_103973, miR-335 and PPP6C in CC tissues and cell lines were examined by qRT-PCR. Cell apoptosis was assessed with PI/Annexin-V double staining followed by the analysis of flow cytometry. Cell proliferation was evaluated by MTT and colony formation assays. Interaction between circ_103973 and miR-335, as well as miR-335 and PPP6C, were verified by dual-luciferase reporter assay.\nRESULTS: Circ_103973 was found to be highly expressed in both GSE102686 and GSE113696 datasets as well as in CC tissue samples and cell lines. Higher levels of circ_103973 were correlated to a worse outcome of CC patients. Knockdown of circ_103973 significantly promoted CC cell apoptosis and inhibited CC cell proliferation in vitro. Mechanistically, we demonstrated that circ_103973 served as a sponge of miR-335, which directly targeted PPP6C in CC cells. miR-335 was found to be decreased in CC, while PPP6C was found to be increased in CC. Moreover, anti-miR-335 could reverse the inhibitory effects of circ_103973 knockdown on CC cell proliferation, and this phenomenon could be blocked by si-PPP6C.\nCONCLUSION: Circ_103973 promoted CC cell proliferation in vitro by physically binding miR-335, which further targeted and regulated PPP6C.", "claims": [{"id": "claim-method", "statement": "Statistical analysis was conducted on SPSS v20 software (IBM Corp., Armonk, NY, USA) using one-way analysis of variance.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32184614", "locator": "sections[13] \"Statistical Analysis\" paragraph 2", "quote": "Statistical analysis was conducted on SPSS v20 software (IBM Corp., Armonk, NY, USA) using one-way analysis of variance."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Two GEO circRNA expression profiles (GSE102686, GSE113696) were downloaded to analyze the differentially expressed circRNAs using bioinformatics methods.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32184614", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "Two GEO circRNA expression profiles (GSE102686, GSE113696) were downloaded to analyze the differentially expressed circRNAs using bioinformatics methods."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:32184614"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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