LPPR4 promotes peritoneal metastasis via Sp1/integrin α/FAK signaling in gastric cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:32266108", "kind": "science-record", "revision": 3, "content_hash": "sha256:619a45767f47e2c3ba7e2ae2a3228b5af09b94018591c85e3ebbd38aa83e595d", "created_at": "2026-11-01T17:33:31Z", "updated_at": "2026-11-01T21:23:05Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "partial", "title": "LPPR4 promotes peritoneal metastasis via Sp1/integrin α/FAK signaling in gastric cancer.", "doi": null, "pmid": "32266108", "authors": ["Dan Zang", "Chuang Zhang", "Ce Li", "Yibo Fan", "Zhi Li", "Kezuo Hou", "Xiaofang Che", "Yunpeng Liu", "Xiujuan Qu"], "journal": "American journal of cancer research", "year": 2020, "abstract": "Gastric cancer (GC) is one of the most common malignancies which has high incidence and mortality worldwide. Peritoneal dissemination is the main route of metastasis in advanced GC. However, few reliable diagnostic or prognostic biomarkers are available for peritoneal metastasis of GC. This study aimed to investigate the effect of lipid phosphate phosphatase-related protein type 4 (LPPR4) on the prognosis of peritoneal metastasis in GC, so as to explore the underlying molecular mechanisms and clinical significance of the process. Differentially expressed genes (DEGs) between tumor tissues and adjacent normal tissues were identified. The prognostic values of the DEGs were tested in two independent cohorts (TCGA-STAD cohort and GSE62254 cohort). Eight DEGs including LPPR4 with prognostic value in GC peritoneal metastasis were identified. The expression of LPPR4 increased in peritoneal metastasis of GC tissues, and high LPPR4 expression was associated with poor overall survival in GC. Loss- and gain-of functional experiments were performed to reveal that LPPR4 could promote the migration, invasion and adhesion abilities of GC cells in vitro. Tumor peritoneal dissemination was investigated in a mouse model to reveal that LPPR4 could promote peritoneal metastasis of GC cells in vivo. According to the Kyoto Encyclopedia of Genes and Genomics (KEGG) and gene set enrichment analysis (GSEA), LPPR4 was found to be related to focal adhesion, cell adhesion molecules (CAMs) and ECM-receptor interaction pathways. LPPR4 knockdown significantly inhibited the expression of integrin α1, integrin α2, integrin α5, integrin α6, integrin α7, p-FAK, p-Akt, p-Src and MMP2. Moreover, this process was regulated by the Specificity Protein 1 (Sp1) transcription factor. Taken together, LPPR4 plays an essential role in promoting peritoneal metastasis of GC through Sp1/integrin α/FAK signaling, and acts as a novel biomarker of prognosis of GC peritoneal metastasis. The results suggest that LPPR4 may serve as a new therapeutic target for patients with GC peritoneal metastasis.", "claims": [{"id": "claim-method", "statement": "According to the Kyoto Encyclopedia of Genes and Genomics (KEGG) and gene set enrichment analysis (GSEA), LPPR4 was found to be related to focal adhesion, cell adhesion molecules (CAMs) and ECM-receptor interaction pathways.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32266108", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "According to the Kyoto Encyclopedia of Genes and Genomics (KEGG) and gene set enrichment analysis (GSEA), LPPR4 was found to be related to focal adhesion, cell adhesion molecules (CAMs) and ECM-receptor interaction pathways."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The prognostic values of the DEGs were tested in two independent cohorts (TCGA-STAD cohort and GSE62254 cohort).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32266108", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "The prognostic values of the DEGs were tested in two independent cohorts (TCGA-STAD cohort and GSE62254 cohort)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Eight DEGs including LPPR4 with prognostic value in GC peritoneal metastasis were identified.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:32266108", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Eight DEGs including LPPR4 with prognostic value in GC peritoneal metastasis were identified."}], "state": "complete"}], "gaps": ["doi"]}, "experiment": {"state": "complete", "objective": "Eight DEGs including LPPR4 with prognostic value in GC peritoneal metastasis were identified.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:32266108"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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