Diagnostic model of combined ceRNA and DNA methylation related genes in esophageal carcinoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:32266120", "kind": "science-record", "revision": 6, "content_hash": "sha256:13665077e051c08058f86a9e779357d9bf8873811e1c14729044cb3dc212f012", "created_at": "2026-11-01T15:40:59Z", "updated_at": "2026-11-01T21:23:05Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Diagnostic model of combined ceRNA and DNA methylation related genes in esophageal carcinoma.", "doi": "10.7717/peerj.8831", "pmid": "32266120", "authors": ["Xiaojiao Guan", "Yao Yao", "Guangyao Bao", "Yue Wang", "Aimeng Zhang", "Xinwen Zhong"], "journal": "PeerJ", "year": 2020, "abstract": "Esophageal cancer is a common malignant tumor in the world, and the aim of this study was to screen key genes related to the development of esophageal cancer using a variety of bioinformatics analysis tools and analyze their biological functions. The data of esophageal squamous cell carcinoma from the Gene Expression Omnibus (GEO) were selected as the research object, processed and analyzed to screen differentially expressed microRNAs (miRNAs) and differential methylation genes. The competing endogenous RNAs (ceRNAs) interaction network of differentially expressed genes was constructed by bioinformatics tools DAVID, String, and Cytoscape. Biofunctional enrichment analysis was performed using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). The expression of the screened genes and the survival of the patients were verified. By analyzing GSE59973 and GSE114110, we found three down-regulated and nine up-regulated miRNAs. The gene expression matrix of GSE120356 was calculated by Pearson correlation coefficient, and the 11696 pairs of ceRNA relation were determined. In the ceRNA network, 643 lncRNAs and 147 mRNAs showed methylation difference. Functional enrichment analysis showed that these differentially expressed genes were mainly concentrated in the FoxO signaling pathway and were involved in the corresponding cascade of calcineurin. By analyzing the clinical data in The Cancer Genome Atlas (TCGA) database, it was found that four lncRNAs had an important impact on the survival and prognosis of esophageal carcinoma patients. QRT-PCR was also conducted to identify the expression of the key lncRNAs (RNF217-AS1, HCP5, ZFPM2-AS1 and HCG22) in ESCC samples. The selected key genes can provide theoretical guidance for further research on the molecular mechanism of esophageal carcinoma and the screening of molecular markers.", "claims": [{"id": "claim-method", "statement": "With the survival time of all esophageal cancers in the TCGA database, the Survival package in the R language was used to perform survival analysis on the differential lncRNA (Shi et al., 2020b).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32266120", "locator": "sections[11] \"Identification of prognostic lncRNAs from ceRNA network\" paragraph 2", "quote": "With the survival time of all esophageal cancers in the TCGA database, the Survival package in the R language was used to perform survival analysis on the differential lncRNA (Shi et al., 2020b)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The following information was supplied regarding data availability:Data is available at NCBI GEO: GSE59973, GSE114110, GSE120356 and GSE52826.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32266120", "locator": "sections[27] \"Data Availability\" paragraph 2", "quote": "The following information was supplied regarding data availability:Data is available at NCBI GEO: GSE59973, GSE114110, GSE120356 and GSE52826."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:32266120"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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