Prognostic Value of ANXA8 in Gastric Carcinoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:32284751", "kind": "science-record", "revision": 9, "content_hash": "sha256:1484f7253ca22da57377ed9f75290e27de196152696889256060eec7ced6ce90", "created_at": "2026-11-01T15:40:59Z", "updated_at": "2026-11-01T22:44:44Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Prognostic Value of ANXA8 in Gastric Carcinoma.", "doi": "10.7150/jca.40010", "pmid": "32284751", "authors": ["Fangqi Ma", "Xiaowei Li", "Haiming Fang", "Yueping Jin", "Qin Sun", "Xuejun Li"], "journal": "Journal of Cancer", "year": 2020, "abstract": "Gastric carcinoma (GC) remains one of the most common and deadly cancers worldwide. In China, the incidence and mortality rates related to GC were quite high. Annexin A8 (ANXA8) is a member of the annexins family of calcium-dependent membrane phospholipid binding proteins. According to recent research, the up-regulation of ANXA8 is closely associated with various types of tumors. However, the specific role of ANXA8 in GC remains unclear. In our study, we explored the prognostic value of ANXA8 in GC. Here, with the data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets (GSE19826 and GSE13861) analyzed, we further performed quantitative real-time polymerase chain reaction (qRT-PCR) using 58 pairs of fresh-frozen tissues. We also subjected 152 pairs of formalin-fixed, paraffin-embedded GC tumor tissues from patients, and the adjacent normal gastric tissues (ANGTs) to immunohistochemical (IHC) analysis. Hence, we found an elevated expression of ANXA8 in tumor tissues with bioinformatics analyses, qRT-PCR, western blot and IHC. Over-expression of ANXA8 was strongly correlated with TNM stages and differentiation grades. Kaplan-Meier and cox proportional-hazard analyses showed that the increased expression of ANXA8 was strongly associated with overall survival (OS) and disease-free survival (DFS) in GC patients. Moreover, we found that ANXA8 is an independent prognostic factor of GC patients' OS and DFS. In brief, those results suggest that ANXA8 can act as an oncogene of GC development and can serve as a potential prognostic biomarker for GC treatment.", "claims": [{"id": "claim-method", "statement": "To determine the prognostic value of ANXA8 in GC patients, we performed Kaplan-Meier (KM) analysis.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32284751", "locator": "sections[12] \"Elevated expression of ANXA8 indicated poor prognosis in GC patients\" paragraph 2", "quote": "To determine the prognostic value of ANXA8 in GC patients, we performed Kaplan-Meier (KM) analysis."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The expression data and corresponding clinical information for GC was obtained from The Cancer Genome Atlas (TCGA) (http://gdc.cancer.gov), Gene Expression Omnibus (GEO) GSE19826 and GSE13861 (http://www.ncbi.nlm.nih.gov/geo) datasets.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32284751", "locator": "sections[3] \"High-throughput data processing\" paragraph 2", "quote": "The expression data and corresponding clinical information for GC was obtained from The Cancer Genome Atlas (TCGA) (http://gdc.cancer.gov), Gene Expression Omnibus (GEO) GSE19826 and GSE13861 (http://www.ncbi.nlm.nih.gov/geo) datasets."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:32284751"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE13861"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE13861"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:32284751", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.7150/jca.40010", "sha256": "sha256:aded03670d48e55ea4cd4977153dc736fc4ff29d85eefaa49f4a6445f0e3774f", "bytes": 18422, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.7150/jca.40010", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC7150447", "source": "europe_pmc_fulltext", "coverage": "whole_body_without_references", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE13861", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE13861", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE13861", "version": null, "note": "The expression data and corresponding clinical information for GC was obtained from The Cancer Genome Atlas (TCGA) (http://gdc.cancer.gov), Gene Expression Omnibus (GEO) GSE19826 and GSE13861 (http://www.ncbi.nlm.nih.gov/geo) datasets.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The expression data and corresponding clinical information for GC was obtained from The Cancer Genome Atlas (TCGA) (http://gdc.cancer.gov), Gene Expression Omnibus (GEO) GSE19826 and GSE13861 (http://www.ncbi.nlm.nih.gov/geo) datasets.", "access_statement_kind": "repository_route_statement", "sample_accessions": ["GSM348332", "GSM348333", "GSM348334", "GSM348335", "GSM348336", "GSM348337", "GSM348338", "GSM348339", "GSM348340", "GSM348341", "GSM348342", "GSM348343", "GSM348344", "GSM348345", "GSM348346", "GSM348347", "GSM348348", "GSM348349", "GSM348350", "GSM348351", "GSM348352", "GSM348353", "GSM348354", "GSM348355", "GSM348356", "GSM348357", "GSM348358", "GSM348359", "GSM348360", "GSM348361", "GSM348362", "GSM348363", "GSM348364", "GSM348365", "GSM348366", "GSM348367", "GSM348368", "GSM348369", "GSM348370", "GSM348371", "GSM348372", "GSM348373", "GSM348374", "GSM348375", "GSM348376", "GSM348377", "GSM348378", "GSM348379", "GSM348380", "GSM348381", "GSM348382", "GSM348383", "GSM348384", "GSM348385", "GSM348386", "GSM348387", "GSM348388", "GSM348389", "GSM348390", "GSM348391", "GSM348392", "GSM348393", "GSM348394", "GSM348395", "GSM348396", "GSM348397", "GSM348398", "GSM348399", "GSM348400", "GSM348401", "GSM348402", "GSM348403", "GSM348404", "GSM348405", "GSM348406", "GSM348407", "GSM348408", "GSM348409", "GSM348410", "GSM348411", "GSM348412", "GSM348413", "GSM348414", "GSM348415", "GSM348416", "GSM348417", "GSM348418", "GSM348419", "GSM348420", "GSM348421"], "sample_count": 90, "group_sizes": null, "group_sizes_basis": null, "platform": "6884", "taxon": "Homo sapiens", "title": "Gene expression signature-based novel prognostic risk score in gastric cancer", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE19826", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE19826", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE19826", "version": null, "note": "The expression data and corresponding clinical information for GC was obtained from The Cancer Genome Atlas (TCGA) (http://gdc.cancer.gov), Gene Expression Omnibus (GEO) GSE19826 and GSE13861 (http://www.ncbi.nlm.nih.gov/geo) datasets.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The expression data and corresponding clinical information for GC was obtained from The Cancer Genome Atlas (TCGA) (http://gdc.cancer.gov), Gene Expression Omnibus (GEO) GSE19826 and GSE13861 (http://www.ncbi.nlm.nih.gov/geo) datasets.", "access_statement_kind": "repository_route_statement", "sample_accessions": ["GSM495051", "GSM495052", "GSM495053", "GSM495054", "GSM495055", "GSM495056", "GSM495057", "GSM495058", "GSM495059", "GSM495060", "GSM495061", "GSM495062", "GSM495063", "GSM495064", "GSM495065", "GSM495066", "GSM495067", "GSM495068", "GSM495069", "GSM495070", "GSM495071", "GSM495072", "GSM495073", "GSM495074", "GSM495075", "GSM495076", "GSM495077"], "sample_count": 27, "group_sizes": null, "group_sizes_basis": null, "platform": "570", "taxon": "Homo sapiens", "title": "Expression data from gastric cancer", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}], "gaps": ["asset:data:geo:GSE13861 not downloaded", "asset:data:geo:GSE13861 licence not read", "asset:data:geo:GSE19826 not downloaded", "asset:data:geo:GSE19826 licence not read"]}, "analysis": {"state": "partial", "methods": [{"id": "method-1", "family": "survival_analysis", "specific": "kaplan_meier", "label": "Survival analysis", "dsl_operations": ["survival_km"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["time_to_event_with_censoring"], "evidence": {"score": 1.0, "band": "verbatim", "quote": "To determine the prognostic value of ANXA8 in GC patients, we performed Kaplan-Meier (KM) analysis."}}, {"id": "method-2", "family": "group_comparison", "specific": "student_t_test", "label": "Group comparison", "dsl_operations": [], "missing_operations": ["student_t"], "expressible": false, "parameters": {}, "data_shapes": ["two_or_more_groups_continuous"], "evidence": {"score": 0.8, "band": "high", "quote": "Student's t-test"}}, {"id": "method-3", "family": "survival_analysis", "specific": "cox_proportional_hazards", "label": "Survival analysis", "dsl_operations": ["cox_ph"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["time_to_event_with_censoring"], "evidence": {"score": 0.8, "band": "high", "quote": "Cox proportional hazards"}}, {"id": "method-4", "family": "differential_expression", "specific": "edger", "label": "Differential expression analysis", "dsl_operations": ["normalise_counts", "differential_expression_fit", "fdr_bh"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["count_matrix_with_condition"], "evidence": {"score": 0.8, "band": "high", "quote": "edgeR"}}, {"id": "method-5", "family": "differential_expression", "specific": "limma_voom", "label": "Differential expression analysis", "dsl_operations": ["normalise_counts", "differential_expression_fit", "fdr_bh"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["count_matrix_with_condition"], "evidence": {"score": 0.8, "band": "high", "quote": "limma"}}, {"id": "method-6", "family": "contingency_analysis", "specific": "chi_square", "label": "Categorical (contingency table) analysis", "dsl_operations": ["chi_square_test"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["categorical_cross_tabulation"], "evidence": {"score": 0.8, "band": "high", "quote": "χ2"}}], "targets": [], "gaps": ["method.reader_differs_from_extractor"]}, "capability": {"state": "complete", "requirements": [{"capability_id": "capability:computational_analysis", "label": "Computational analysis platform", "assay_families": ["meta_analysis", "machine_learning", "survival_analysis", "statistical_regression", "pca", "molecular_docking", "network_analysis", "differential_expression", "gwas"], "availability_state": "owned", "tier_unlocked": "R1", "candidate": "Automated Assays Laravel application and simulated adapters"}], "gaps": []}, "readiness": {"state": "complete", "tier": "R1", "blocking_axis": "none", "blocked_on": "nothing:replicable_now", "axes": {"method": {"tier": "R1", "expressible": true, "required_operations": ["survival_km"], "missing_operations": [], "basis": "The paper names the method it used and the corpus's method extractor maps it onto the DSL catalogue: kaplan_meier."}, "capability": {"tier": "R1", "capability_id": "capability:computational_analysis", "availability_state": "owned", "basis": "The computational analysis is owned. The physical bench the paper used is not, so a physical validation cannot be run here."}, "data": {"tier": "R1", "reachable": true, "accessions": ["GSE19826", "GSE13861"], "resolved_accessions": ["GSE19826", "GSE13861"], "statements": ["The expression data and corresponding clinical information for GC was obtained from The Cancer Genome Atlas (TCGA) (http://gdc.cancer.gov), Gene Expression Omnibus (GEO) GSE19826 and GSE13861 (http://www.ncbi.nlm.nih.gov/geo) datasets."], "basis": "The paper names GSE19826, GSE13861 in Gene Expression Omnibus (GEO) and the accession resolves in the repository. No dataset was downloaded and no licence was read."}}, "can_run": {"dsl_operations": ["survival_km"], "assets": ["source:pmid:32284751", "data:geo:GSE13861"], "steps": ["run_analysis"]}, "must_build": {"dsl_operations": [], "converters": [], "readers": [], "capabilities": [], "data_gaps": ["data:geo:GSE13861"]}, "gaps": []}, "review": {"state": "partial", "machine_critique": {"kind": "extraction_review", "reader": {"model": "MiniMax-M3.1-Flash", "provider": "minimax", "provider_family": "minimax", "read_at": "2026-10-31T18:19:07Z", "independence": "single_reader"}, "evidence_check": {"method": "verbatim", "availability": null}, "calls": 3, "usage": {"prompt_tokens": 14645, "completion_tokens": 1135, "cached_tokens": 784}}, "human_critique": null, "stages": [{"id": "extraction", "state": "complete", "reader": "MiniMax-M3.1-Flash", "provider": "minimax"}], "gaps": ["review.independent_reader", "review.machine_critique", "review.human_critique"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "pmid:32284751", "type": "paper", "label": "pmid:32284751"}, {"id": "source:pmid:32284751", "type": "manuscript", "label": "PMID 32284751 full text"}, {"id": "data:geo:GSE13861", "type": "dataset", "label": "GEO GSE13861"}], "activities": [{"id": "activity-reading-sci-2026-11-01", "type": "classification", "used": [{"id": "pmid:32284751", "type": "paper", "label": "pmid:32284751"}, {"id": "source:pmid:32284751", "type": "manuscript", "label": "PMID 32284751 full text"}, {"id": "data:geo:GSE13861", "type": "dataset", "label": "GEO GSE13861"}], "agent": "agent:corpus-assay-engine"}, {"id": "activity-read-32284751", "type": "extraction", "used": ["source:pmid:32284751"], "agent": "agent:minimax-reader"}, {"id": "activity-resolve-GSE13861", "type": "accession_resolution", "used": ["data:geo:GSE13861"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}, {"id": "agent:minimax-reader", "type": "software", "label": "MiniMax literature reader"}], "source_run_id": "reading-sci-2026-11-01", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "pmid:32284751", "parent_hash": "sha256:b2ade00a54f63fbca0d3f627d0379c13d19113c4fa2b01fccfa74ea5959ef9ea", "fork_of": null, "generation": 8}, "gaps": ["asset:data:geo:GSE13861 licence not read", "asset:data:geo:GSE13861 not downloaded", "asset:data:geo:GSE19826 licence not read", "asset:data:geo:GSE19826 not downloaded", "execution.not_run", "method.reader_differs_from_extractor", "review.human_critique", "review.independent_reader", "review.machine_critique"], "assays": [{"role": "data_generation", "definition_id": "ihc", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["e adjacent normal gastric tissues (ANGTs) to immunohistochemical (IHC) analysis. Hence, we found an elevated", "tric tissues (ANGTs) to immunohistochemical (IHC) analysis. Hence, we found an elevated expre", "ormatics analyses, qRT-PCR, western blot and IHC. Over-expression of ANXA8 was strongly corre"], "order_basis": "paper_text_order:909", "id": "assay:1"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["ssues with bioinformatics analyses, qRT-PCR, western blot and IHC. Over-expression of ANXA8 was strong"], "order_basis": "paper_text_order:1049", "id": "assay:2"}, {"role": "analysis", "definition_id": "survival_analysis", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["survival_km"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["To determine the prognostic value of ANXA8 in GC patients, we performed Kaplan-Meier (KM) analysis."], "order_basis": "consumes_earlier_output", "id": "assay:3"}]}