miR‑95 promotes osteosarcoma growth by targeting SCNN1A.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:32323794", "kind": "science-record", "revision": 8, "content_hash": "sha256:f12a728c3114307ce1f781153c98371e2aacc86f3cbf685a5368d6ea209210d6", "created_at": "2026-11-01T15:41:00Z", "updated_at": "2026-11-01T22:44:46Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "miR‑95 promotes osteosarcoma growth by targeting SCNN1A.", "doi": "10.3892/or.2020.7514", "pmid": "32323794", "authors": ["Yannan Geng", "Shaorong Zhao", "Yutao Jia", "Gang Xia", "Huiming Li", "Zhao Fang", "Quan Zhang", "Rong Tian"], "journal": "Oncology reports", "year": 2020, "abstract": "Osteosarcoma (OS) is a common malignant bone tumor, presenting particularly in children and young adults, and accounts for approximately 19% of all malignant bone cancers. Despite advances in OS treatment, long‑term prognosis remains poor. miRNAs are non‑coding single‑stranded RNAs ~22 nucleotides in length. Increasing evidence suggests that numerous miRNAs may play critical roles in tumorigenesis and tumor progression; however, the role of miR‑95 in OS has not been examined. In the present study, we investigated the role of miR‑95 in OS using in vitro and in vivo models and publicly available expression data. Our findings indicate that abnormal miR‑95 expression occurs in OS, according to the Gene Expression Omnibus (GEO) database. The miR‑95 inhibitor reduced cell proliferation and promoted apoptosis in OS cell lines as detected by EdU staining, TUNEL staining and flow cytometry. Furthermore, a dual luciferase reporter assay revealed that miR‑95 regulates the cell cycle of OS cells and apoptosis by targeting sodium channel epithelial 1α subunit (SCNN1A). Additionally, miR‑95 antagomir suppressed the growth of U2OS xenograft tumors in a mouse model. In summary, our results suggest that miR‑95 induces OS growth in vitro and in vivo by targeting SCNN1A. Our results help clarify the mechanism underlying the miR‑95‑mediated effects on OS tumor growth, thus potentially establishing it as a diagnostic target.", "claims": [{"id": "claim-method", "statement": "Significant differences were analyzed by the Student's t-test or one-way analysis of variance (ANOVA) followed by Tukey's post-hoc test. Spearman's correlation analysis was also performed.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32323794", "locator": "sections[13] \"Statistical analysis\" paragraph 2", "quote": "Significant differences were analyzed by the Student's t-test or one-way analysis of variance (ANOVA) followed by Tukey's post-hoc test. 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In summary, our results suggest that miR‑95", "the role of miR‑95 in OS using in vitro and in vivo models and publicly available expression dat", "t that miR‑95 induces OS growth in vitro and in vivo by targeting SCNN1A. Our results help clarif", "sed the growth of U2OS xenograft tumors in a mouse model. In summary, our results suggest that"], "order_basis": "paper_text_order:576", "id": "assay:1"}, {"role": "data_generation", "definition_id": "apoptosis_assay", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["itor reduced cell proliferation and promoted apoptosis in OS cell lines as detected by EdU staining", "‑95 regulates the cell cycle of OS cells and apoptosis by targeting sodium channel epithelial 1α su"], "order_basis": "paper_text_order:816", "id": "assay:2"}, {"role": "data_generation", "definition_id": "flow_cytometry", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["detected by EdU staining, TUNEL staining and flow cytometry. Furthermore, a dual luciferase reporter ass"], "order_basis": "paper_text_order:891", "id": "assay:3"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["y, miR‑95 antagomir suppressed the growth of U2OS xenograft tumors in a mouse model. In summar", "n Type Culture Collection (ATCC). Cells were cultured in DMEM containing 10% FBS and 100 U/ml penicil", "culture and transfection Human OS cell lines U2OS, MG-63, and Saos-2 were obtained from the Am"], "order_basis": "paper_text_order:1141", "id": "assay:4"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["Materials and methods Cell culture and transfection Human OS cell lines U2OS, MG-63, and Saos-2", "h the manufacturer's instructions. Following transfection at 48 h, subsequent experimentation was perf"], "order_basis": "paper_text_order:1485", "id": "assay:5"}, {"role": "analysis", "definition_id": "group_comparison", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["Significant differences were analyzed by the Student's t-test or one-way analysis of variance (ANOVA) followed by Tukey's post-hoc test. Spearman's correlation analysis was also performed."], "order_basis": "consumes_earlier_output", "id": "assay:6"}]}