ZWINT: A potential therapeutic biomarker in patients with glioblastoma correlates with cell proliferation and invasion.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:32323832", "kind": "science-record", "revision": 7, "content_hash": "sha256:f58ac2e51ddfc4b18627e248f91cdae224ba9a7d4a87cc8f8736bd0d4ae190cc", "created_at": "2026-11-01T15:41:00Z", "updated_at": "2026-11-01T22:01:32Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "ZWINT: A potential therapeutic biomarker in patients with glioblastoma correlates with cell proliferation and invasion.", "doi": "10.3892/or.2020.7573", "pmid": "32323832", "authors": ["Li Yang", "Na Han", "Xiaoxi Zhang", "Yangmei Zhou", "Rui Chen", "Mengxian Zhang"], "journal": "Oncology reports", "year": 2020, "abstract": "Glioblastoma (GBM) is the most aggressive primary intracranial tumor in adults. Chemoradiotherapy resistance and recurrence after surgery are the main malignant progression factors, leading to a high mortality rate. Therefore, the exploration of novel biomarkers and molecular mechanisms of GBM is urgent. Differentially expressed genes (DEGs) of GBM were screened in a TCGA dataset. Homo sapiens ZW10 interacting kinetochore protein (ZWINT) was found to be upregulated in GBM, which was confirmed by immunohistochemical staining of a tissue microarray. Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed using the Database for Annotation, Visualization and Integrated Discovery (DAVID) database. A protein‑protein interaction (PPI) network was established by the STRING database, and hub genes were visualized by Cytoscape. The correlation results were verified with the GSE15824 dataset. Bioinformatic analysis confirmed that ZWINT was significantly positively correlated with kinetochore protein NDC80 homolog (NDC80), serine/threonine‑protein kinase PLK1 (PLK1) and spindle and kinetochore associated complex subunit 1 (SKA1) and together are involved in regulating mitosis and the cell cycle of GBM. ZWINT expression was knocked down in U251 and U87 MG GBM cells by lentiviral vectors carrying a small hairpin RNA (shRNA) targeting ZWINT. The effect of ZWINT silencing on cell proliferation, invasion and apoptosis was determined by the Celigo assay, MTT assay, Transwell assay, flow cytometry and caspase‑3/7 assay in vitro. A subcutaneous xenograft tumor model was established to explore the influence of ZWINT knockdown on GBM growth in vivo. Our preliminary study demonstrated that ZWINT knockdown effectively inhibited proliferation and invasion and induced apoptosis of GBM cells and notably suppressed GBM growth in vivo. Therefore, we speculate that ZWINT may be a potential therapeutic biomarker for GBM, with NDC80 and PLK1 conjointly involved in regulating cell division and the mitotic cell cycle.", "claims": [{"id": "claim-method", "statement": "Student's t-test was used to compare data between two groups.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32323832", "locator": "sections[17] \"Statistical analysis\" paragraph 2", "quote": "Student's t-test was used to compare data between two groups."}], "state": "complete"}, {"id": "claim-data-access", "statement": "All datasets generated during our present study are available through data mining from public gene databases.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32323832", "locator": "sections[31] \"Availability of data and materials\" paragraph 2", "quote": "All datasets generated during our present study are available through data mining from public gene databases."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:32323832"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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