Ultraviolet Photodissociation of Tryptic Peptide Backbones at 213 nm.

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We obtained 15 178 unique high-confidence peptide UVPD spectrum matches by recording a reference beam-type collision-induced dissociation (HCD) spectrum of each precursor, ensuring that our investigation includes a broad selection of peptides, including those that fragmented poorly by UVPD. Type a, b, and y ions were most prominent in UVPD spectra, and median sequence coverage ranged from 5.8% (at 5 ms laser excitation time) to 45.0% (at 100 ms). Overall, the sequence fragment intensity remained relatively low (median: 0.4% (5 ms) to 16.8% (100 ms) of total intensity), and the remaining precursor intensity, high. The sequence coverage and sequence fragment intensity ratio correlated with the precursor charge density, suggesting that UVPD at 213 nm may suffer from newly formed fragments sticking together due to noncovalent interactions. The UVPD fragmentation efficiency therefore might benefit from supplemental activation, as was shown for ETD. Aromatic amino acids, most prominently tryptophan, facilitated UVPD. This points to aromatic tags as possible enhancers of UVPD. Data are available via ProteomeXchange with identifier PXD018176 and on spectrumviewer.org/db/UVPD-213nm-trypPep.", "claims": [{"id": "claim-method", "statement": "We split our PSMs by the charge state and evaluated the correlation to the sequence coverage by linear regression fitting (Figure 4A).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32352297", "locator": "sections[10] \"Influence of the Charge State on UVPD\" paragraph 2", "quote": "We split our PSMs by the charge state and evaluated the correlation to the sequence coverage by linear regression fitting (Figure 4A)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The mass spectrometry raw files, peak lists, search engine results, and FASTA files have been deposited to the ProteomeXchange Consortium via the PRIDE17 partner repository with the PXD018176 data set identifier and 10.6019/PXD018176. 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