PKMYT1 as a Potential Target to Improve the Radiosensitivity of Lung Adenocarcinoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:32411179", "kind": "science-record", "revision": 7, "content_hash": "sha256:9f809c2d074746707b5df7334025c5a6b412ad5f0f54a431e6e26fb95c35c0d2", "created_at": "2026-11-01T15:41:01Z", "updated_at": "2026-11-01T22:01:38Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "PKMYT1 as a Potential Target to Improve the Radiosensitivity of Lung Adenocarcinoma.", "doi": "10.3389/fgene.2020.00376", "pmid": "32411179", "authors": ["Huan-Ping Long", "Jia-Qing Liu", "Yang-Yang Yu", "Qiao Qiao", "Guang Li"], "journal": "Frontiers in genetics", "year": 2020, "abstract": "OBJECTIVE: This article is dedicated to finding important genes related to the prognosis of lung adenocarcinoma (LUAD), looking for a new gene that may affect tumor radiosensitivity, and conducting basic experiments to verify the relationship between this gene and the radiosensitivity of LUAD.\nMETHODS: The gene expression profiles GSE32863, GSE33532, and GSE43458 were obtained from NCBI-GEO. GEO2R and a Venn diagram were used to identify upregulated genes. STRING and Cytoscape were applied to develop a protein-protein interaction network (PPI) and analyze the modules. The Database for Annotation, Visualization and Integrated Discovery (DAVID) was used to process the GO and KEGG pathway analysis. The Kaplan Meier plotter and Gene Expression Profiling Interactive Analysis (GEPIA) were applied to get the significant prognostic information and differential expression between LUAD tissues and normal lung tissues. Western blotting and Q-PCR were used to detect the expression of PKMYT1 in tissues. Small interfering RNAs (siRNAs) were used to knockdown PKMYT1. The colony survival experiment was used to assess the effect of PMYT1 on the radiosensitivity of tumor cells. Cell cycle analysis was used to assess cell cycle distribution.\nRESULTS: We identified 14 genes (PKMYT1, TTK, CHEK1, CDC20, PTTG1, MCM2, CDC25C, MCM4, CCNB1, CDC45, MAD2L1, CCNB2, BUB1, and CCNA2) that are important for LUAD and may be potential therapeutic targets. We confirmed that PKMYT1 is highly expressed in LUAD and firstly demonstrated that artificially silencing the expression of PKMYT1 can abrogate IR-induced G2/M phase arrest and increase the sensitivity of cancer cells to radiation.\nCONCLUSION: In summary, we obtained 14 core genes related to the poor prognosis of LUAD via bioinformatical analysis. We identified that PKMYT1 was significantly upregulated in LUAD tissues and firstly demonstrated that knockdown of PKMYT1 can eliminate the radiation-induced G2/M arrest, resulting in a lower survival rate for cells receiving radiation therapy. Our findings suggested that PKMYT1 is a promising target to improve the radiosensitivity of LUAD.", "claims": [{"id": "claim-method", "statement": "Kaplan Meier plotter was used to analyze the overall survival (OS) of 72 hub genes.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32411179", "locator": "sections[16] \"Identification of Core Genes\" paragraph 2", "quote": "Kaplan Meier plotter was used to analyze the overall survival (OS) of 72 hub genes."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets generated for this study can be found in the data is available at NCBI GEO under accession numbers GSE33532, GSE32863, and GSE43458.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32411179", "locator": "sections[24] \"Data Availability Statement\" paragraph 2", "quote": "The datasets generated for this study can be found in the data is available at NCBI GEO under accession numbers GSE33532, GSE32863, and GSE43458."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:32411179"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE32863"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE32863"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:32411179", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.3389/fgene.2020.00376", "sha256": 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LUAD and normal lung tissues.", "rows": [["Categories", "Genes names"], ["Genes with significantly high expression in LUAD (P < 0.05)", "MAD2L1 PKMYT1 KIF2C TTK CCNA2 BUB1 KIF20A CKAP2L BIRC5 CCNB2 EXO1 CDCA3 CDKN3 CDC20 OIP5 HJURP MCM2 MCM10 PRC1 MELK KIF15 NCAPG PTTG1 TK1 KIF4A UBE2C FOXM1 AURKA KIFC1 CEP55 CDC25C TYMS ASF1B KIAA0101 CENPF DLGAP5 CHEK1 CDCA5 ZWINT ANLN CDC45 GINS2 SHCBP1 NEK2 AURKB UBE2T CCNB1 TPX2 MCM4 TOP2A PBK ASPM CDCA8"], ["Genes without significantly high expression in LUAD (P < 0.05)", "KIF14 EZH2 POLQ TIMELESS RFC4"]], "row_count": 3, "column_count": 2, "numeric_cells": 4, "header_rows": 1, "locator": "section[16]/table-wrap[2]#T3"}}, {"id": "asset:table:pmid:32411179:4", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:0a301d03f686305acf148292a98896b49cd6bd4f3ae916e6b4c522ba9bdfcfc2", "bytes": 632, "access": "open", "state": "verified", "reader": "read_table", "reader_status": 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