Long non-coding RNA LINC00520 promotes the proliferation and metastasis of malignant melanoma by inducing the miR-125b-5p/EIF5A2 axis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:32466797", "kind": "science-record", "revision": 7, "content_hash": "sha256:9818147710a66b785a897b78ffae615c9cbb745e68f97d6cd4f4a1b4bf592f36", "created_at": "2026-11-01T15:41:02Z", "updated_at": "2026-11-01T22:01:42Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Long non-coding RNA LINC00520 promotes the proliferation and metastasis of malignant melanoma by inducing the miR-125b-5p/EIF5A2 axis.", "doi": "10.1186/s13046-020-01599-7", "pmid": "32466797", "authors": ["Wenkang Luan", "Yuting Ding", "Haitao Yuan", "Shaojun Ma", "Hongru Ruan", "Jinlong Wang", "Feng Lu", "Xuefeng Bu"], "journal": "Journal of experimental & clinical cancer research : CR", "year": 2020, "abstract": "BACKGROUND: Long intergenic non-protein coding RNA 520 (LINC00520), a novel identified lncRNA, has been shown to modulate the malignant phenotype of tumor cells in some malignant tumors. However, the exact role and molecular mechanism of LINC00520 in malignant melanoma has not been studied.\nMETHODS: The expression of LINC00520 in melanoma tissues were detected by using RNA-seq analysis and qRT-PCR. Melanoma cases from the public databases (The Cancer Genome Atlas (TCGA), GEO#GSE15605, GEO#GSE34460 and GEO#GSE24996) were included in this study. CCK-8 assay, EdU assay, transwell and scratch wound assay were used to explore the role of LINC00520 in melanoma cells. Luciferase reporter assays, MS2-RIP, RNA pull-down and RNA-ChIP assay were used to demonstrate the molecular biological mechanism of LINC00520 in melanoma.\nRESULTS: We found that LICN00520 was found to be overexpressed in melanoma tissue. High expression of LICN00520 is a risk factor for the prognosis of melanoma patients. LINC00520 promotes the proliferation, invasion and migration of melanoma cells. LICN00520 exerted its oncogenic role by competitive binding miR-125b-5p to promote Eukaryotic initiation factor 5A2 (EIF5A2) expression. We also showed that LICN00520 promotes the growth and metastasis of melanoma in vivo through regulating miR-125b-5p/EIF5A2 axis.\nCONCLUSIONS: All results elucidated the role and molecular mechanism of LINC00520 in the malignant development of melanoma. LINC00520, a new oncogene in melanoma, maybe serve as a survival biomarkers or therapeutic target for melanoma patients.", "claims": [{"id": "claim-data-access", "statement": "All the data and materials supporting the conclusions were included in the main paper.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32466797", "locator": "sections[36] \"Availability of data and materials\" paragraph 2", "quote": "All the data and materials supporting the conclusions were included in the main paper."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:32466797"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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CCK-8 assay, EdU assay, transwell and scratch woun", "tive ability of MeWo cells was determined by CCK8 assay, and the results were further confirme"], "order_basis": "paper_text_order:640", "id": "assay:2"}, {"role": "data_generation", "definition_id": "animal_model", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.77, "evidence": ["omotes the growth and metastasis of melanoma in vivo through regulating miR-125b-5p/EIF5A2 axis."], "order_basis": "paper_text_order:1379", "id": "assay:3"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["Materials Additional file 1: Figure S1. (A) Western blots identified EIF5A2 protein expression changes"], "order_basis": "paper_text_order:7700", "id": "assay:4"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["0 or si-LINC00520 plus miR-125b-5p inhibitor transfected MeWo cells, GAPDH was used as a control. (B)", "and the results were further confirmed by co-transfection miR-125b-5p inhibitor. 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