Effect of AGER on the biological behavior of non‑small cell lung cancer H1299 cells.

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Therefore, this study aimed to explore the effect of AGER on the biological behavior of non‑small cell lung cancer (NSCLC) H1299 cell line. A microarray‑based gene expression profiling analysis of the GSE27262 dataset from the Gene Expression Omnibus (GEO) database was conducted to identify differentially expressed genes, which were verified using The Cancer Genome Atlas (TCGA) database. The expression of AGER in the normal human lung BEAS‑2B cell line and NSCLC H1299 cell line was examined using reverse transcription‑quantitative PCR. Lentiviral interference and overexpression vectors of AGER were constructed and transfected into H1299 cells using Lipofectamine®. AGER expression and biological properties, including cell viability, apoptosis, migration and invasion abilities, in H1299 cells were investigated using MTT, flow cytometry, wound healing and Transwell assays. AGER was expressed at a low level in NSCLC tissues and H1299 cells (P<0.05). Compared with control cells, AGER overexpression cells displayed decreased cell viability, proliferation, migration and invasion abilities, and significantly increased levels of apoptosis. Furthermore, AGER overexpression increased the expression of Bax and decreased the expression of Bcl‑2 in H1299 cells (P<0.05), and AGER knockdown displayed the opposite effects on H1299 cells. Therefore, AGER overexpression decreased the proliferation, invasion and migration abilities of H1299 cells, and increased apoptosis. The present study suggested that AGER might serve as a potential molecular marker for NSCLC.", "claims": [{"id": "claim-method", "statement": "Comparisons between two groups were analyzed using paired Student's t-test and multigroup comparisons were made using analysis of variance (ANOVA) with Tukey's post hoc test.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32468030", "locator": "sections[13] \"Statistical analysis\" paragraph 2", "quote": "Comparisons between two groups were analyzed using paired Student's t-test and multigroup comparisons were made using analysis of variance (ANOVA) with Tukey's post hoc test."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The dataset (GSE27262) analyzed during the current study is available in the Gene Expression Omnibus (GEO) database (www.ncbi.nlm.nih.gov/geo). 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A microarray‑based gene expression profiling analysis of"], "order_basis": "paper_text_order:371", "id": "assay:1"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["ression vectors of AGER were constructed and transfected into H1299 cells using Lipofectamine®. AGER", "n of Bcl‑2 in H1299 cells (P<0.05), and AGER knockdown displayed the opposite effects on H1299 cell", "Materials and methods Cell culture and transfection The human normal lung BEAS-2B cell line, the", "vitrogen; Thermo Fisher Scientific, Inc.) co-transfected into 293T cells (cell density: 1.5×104) at a"], "order_basis": "paper_text_order:850", "id": "assay:2"}, {"role": "data_generation", "definition_id": "apoptosis_assay", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["ogical properties, including cell viability, apoptosis, migration and invasion abilities, in H1299", "ities, and significantly increased levels of apoptosis. Furthermore, AGER overexpression increased", "tion abilities of H1299 cells, and increased apoptosis. The present study suggested that AGER might", "ties, in H1299 cells were investigated using MTT, flow cytometry, wound healing and Transwell"], "order_basis": "paper_text_order:970", "id": "assay:3"}, {"role": "data_generation", "definition_id": "flow_cytometry", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["in H1299 cells were investigated using MTT, flow cytometry, wound healing and Transwell assays. AGER wa"], "order_basis": "paper_text_order:1060", "id": "assay:4"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["inese Academy of Sciences. BEAS-2B and H1299 cell lines were cultured in RPMI-1640 medium (Sigma-Aldrich; Merck KG", "Gibco; Thermo Fisher Scientific, Inc.), 293T cell line were cultured in Dulbecco's Modified Eagle's Medium (DMEM,", "Sciences. BEAS-2B and H1299 cell lines were cultured in RPMI-1640 medium (Sigma-Aldrich; Merck KGaA)", "isher Scientific, Inc.), 293T cell line were cultured in Dulbecco's Modified Eagle's Medium (DMEM,Gib"], "order_basis": "paper_text_order:2078", "id": "assay:5"}, {"role": "data_generation", "definition_id": "qpcr", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["sing reverse transcription-quantitative PCR (RT-qPCR). Interference efficiency was detected using", ". Interference efficiency was detected using RT-qPCR. Statistical analysis Statistical analyses w", "g reverse transcription-quantitative PCR (RT-qPCR). Interference efficiency was detected using", "nterference efficiency was detected using RT-qPCR. Statistical analysis Statistical analyses w"], "order_basis": "paper_text_order:4422", "id": "assay:6"}, {"role": "analysis", "definition_id": "group_comparison", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["Comparisons between two groups were analyzed using paired Student's t-test and multigroup comparisons were made using analysis of variance (ANOVA) with Tukey's post hoc test."], "order_basis": "consumes_earlier_output", "id": "assay:7"}]}