Comparative Platelet Releasate Proteomic Profiling of Acute Coronary Syndrome versus Stable Coronary Artery Disease.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:32671099", "kind": "science-record", "revision": 7, "content_hash": "sha256:51e78bc4cf22f323b3f0ddfc948aa72c33f16a598f8d7ada04e7e387760405ad", "created_at": "2026-11-01T15:41:06Z", "updated_at": "2026-11-01T22:01:55Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Comparative Platelet Releasate Proteomic Profiling of Acute Coronary Syndrome versus Stable Coronary Artery Disease.", "doi": "10.3389/fcvm.2020.00101", "pmid": "32671099", "authors": ["Patricia B Maguire", "Martin E Parsons", "Paulina B Szklanna", "Monika Zdanyte", "Patrick Münzer", "Madhumita Chatterjee", "Kieran Wynne", "Dominik Rath", "Shane P Comer", "Melanie Hayden", "Fionnuala Ní Áinle", "Meinrad Gawaz"], "journal": "Frontiers in cardiovascular medicine", "year": 2020, "abstract": "Upon activation, platelets release a host of soluble and vesicular signals, collectively termed the \"platelet releasate\" (PR). The contents of this PR play a significant role in haemostasis, inflammation, and pathologic sequelae. Despite this, proteomic studies investigating the PR in coronary artery disease have not been performed. Here, we undertook a comparative label-free quantitative (LFQ) proteomic profiling of the 1 U/ml thrombin-induced PR from 13 acute coronary syndrome vs. 14 stable angina pectoris patients using a tandem mass spectrometry approach. Data are available via ProteomeXchange with identifier PXD009356. 318 PR proteins were identified across both cohorts with 9 proteins found to be differentially released, including tetranectin (CLEC3B), protein disulfide-isomerase-A3 (PDIA3), coagulation factor V (F5), and fibronectin (FN1). Strikingly, these 9 differential proteins were all associated with the gene ontology cellular component term \"extracellular vesicle\" and reduced levels of EVs were detected in the corresponding plasma of ST-segment elevation myocardial infarction (STEMI) patients. Network analysis revealed 3 proteins either reduced (F5; FN1) or absent (CLEC3B) in the PR of STEMI patients that are strongly connected to both the clotting cascade and major druggable targets on platelets. This moderated proteomic signature may prove useful for non-invasive risk assessment of the progression of coronary artery disease. These data further contribute to the growing evidence-base of using the platelet releasate as a predictor of pathological state and disease severity.", "claims": [{"id": "claim-method", "statement": "The alteration in the secretion levels of the remaining 14 proteins (difference between the mean log2 LFQ values >1/1) were evaluated for statistical significance (p < 0.05) utilizing Student's t-test analysis with an FDR value of 0.05 and an S0 correction factor of 0.1, denoted by the black hyperbolic lines (Figure 2C) (24).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32671099", "locator": "sections[7] \"Results\" paragraph 4", "quote": "The alteration in the secretion levels of the remaining 14 proteins (difference between the mean log2 LFQ values >1/1) were evaluated for statistical significance (p < 0.05) utilizing Student's t-test analysis with an FDR value of 0.05 and an S0 correction factor of 0.1, denoted by the black hyperbolic lines (Figure 2C) (24)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Raw MS data has been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD009356.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32671099", "locator": "sections[6] \"Protein ID, Quantification, and Statistical Rationale\" paragraph 2", "quote": "Raw MS data has been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD009356."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:32671099"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:PXD009356"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "blocked", "blocked_on": "method:student_t", "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:PXD009356"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": ["step:run_analysis blocked on method:student_t"]}, "data": {"state": "partial", "assets": [{"id": "source:pmid:32671099", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.3389/fcvm.2020.00101", "sha256": "sha256:90491cbd8b2de90581b294b1caf7825da41e7916b34dd3b38af2ca282ad4afab", "bytes": 36237, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.3389/fcvm.2020.00101", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC7328343", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:PXD009356", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=PXD009356", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "PXD009356", "version": null, "note": "Raw MS data has been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD009356.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "Raw MS data has been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD009356.", "access_statement_kind": "data_availability_statement", "sample_accessions": [], "sample_count": null, "group_sizes": null, "group_sizes_basis": null, "platform": null, "taxon": null, "title": "Comparative platelet releasate proteome profiling of acute coronary syndrome versus stable coronary artery disease", "resolution_checked_at": "2026-10-31T18:35:55Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "asset:table:pmid:32671099:1", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": 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SD)", "27.31 (±4.0)", "25.23 (±2.6)", "28.44 (±4.3)", "0.116"], ["Cardiovascular risk factors"], ["Arterial Hypertension n (%)", "22 (81.5%)", "11 (84.6%)", "11 (78.6%)", "0.686"], ["Hyperlipidemia n (%)", "11 (40.7%)", "5 (38.5%)", "6 (42.9%)", "0.082"], ["Diabetes mellitus n (%)", "8 (29.6%)", "3 (23.1%)", "5 (35.7%)", "0.472"], ["Current smoking n (%)", "6 (22.2%)", "2 (15.4%)", "4 (28.6%)", "0.410"], ["Obesity n (%)", "6 (22.2%)", "1 (7.7%)", "5 (35.7%)", "0.080"], ["Renal function (GFR) (Mean ± SD)", "79.26 (±24.7)", "80.86 (±26.8)", "77.66 (±23.3)", "0.748"], ["Medication on admission"], ["Acetylsalicylic acid n (%)", "11 (40.7%)", "2 (15.4%)", "9 (64.3%)", "0.017"], ["Clopidogrel n (%)", "4 (14.81%)", "0 (0.0%)", "4 (28.6%)", "0.011"], ["Prasugrel n (%)", "1 (3.7%)", "0 (0.0%)", "1 (7.1%)", "0.024"], ["Ticagrelor n (%)", "1 (3.7%)", "0 (0.0%)", "1 (7.1%)", "0.024"], ["Oral anticoagulants n (%)", "3 (11.1%)", "0 (0.0%)", "3 (21.4%)", "0.014"], ["Angiotensin-converting enzyme inhibitors n (%)", "8 (29.6%)", "1 (7.7%)", "7 (50.0%)", "0.011"], ["Angiotensin II receptor antagonists n (%)", "4 (14.8%)", "2 (15.4%)", "2 (14.3%)", "0.372"], ["Beta-blockers n (%)", "13 (48.1%)", "3 (23.1%)", "10 (71.4%)", "0.016"], ["Statins n (%)", "10 (37.0%)", "1 (7.7%)", "9 (64.3%)", "0.004"], ["Lipid profile parameters"], ["Total cholesterol mg/dl (mean ± SD)mmol/l (mean ± SD)", "167.7 (±33.7) 4.4 (±0.9)", "169.0 (±37.0) 4.4 (±1.0)", "166.6 (±32.3) 4.3 (±0.8)", "0.872"], ["LDL-cholesterol mg/dl (mean ± SD)mmol/l (mean ± SD)", "103.3 (±32.1) 2.7 (±0.8)", "106.9 (±39.1) 2.8 (±1.0)", "101.2 (±28.9) 2.6 (±0.7)", "0.721"], ["HDL-cholesterolmg/dl (mean ± SD)mmol/l (mean ± SD)", "44.1 (±15.1) 1.1 (±0.4)", "39.3 (±21.2) 1.1 (±0.5)", "46.0 (±10.3) 1.2 (±0.3)", "0.324"], ["Triglyceridesmg/dl (mean ± SD)mmol/l (mean ± SD)", "138.8 (±67.0) 1.6 (±0.8)", "123.0 (±70.7) 1.4 (±0.8)", "152.0 (±63.7) 1.7 (±0.7)", "0.306"]], "row_count": 26, "column_count": 5, "numeric_cells": 91, "header_rows": 1, "locator": "section[3]/table-wrap[1]#T1"}}, {"id": "asset:table:pmid:32671099:2", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:de4109ced104bb11a93930e4068655a6301e752742d91e4c88f9ce59eee99d4a", "bytes": 2131, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[7]/table-wrap[1]#T2", "version": null, "note": "9 PR proteins differentially released in STEMI vs. SAP.", "structure": {"pmcid": "PMC7328343", "section_index": 7, "section_title": "Results", "row_count": 14, "column_count": 6}, "table": {"label": "Table 2", "caption": "9 PR proteins differentially released in STEMI vs. SAP.", "rows": [["Gene name", "Protein name", "SAP log2 LFQ", "STEMI log2 LFQ", "Difference (mean log2 LFQ values)", "Function"], ["Proteins unique to STEMI"], ["FAH", "Fumarylacetoacetase", "NaN", "22.398", "NaN", "Final enzyme in the tyrosine catabolism pathway. FAH deficiency is associated with Type 1 hereditary tyrosinemia"], ["Proteins unique to SAP"], ["CLEC3B", "Tetranectin", "24.613", "NaN", "NaN", "Binds to Kringle 4 domain of plasminogen. May be involved in the packaging of molecules destined for exocytosis."], ["HSP90AA1", "Heat shock protein HSP 90-alpha", "24.132", "NaN", "NaN", "Molecular chaperone, involved in platelet activation. Extracellularly, mediates immune-regulatory & tissue remodeling."], ["Proteins with increased secretion in STEMI"], ["GPI", "Glucose-6-phosphate isomerase", "24.76", "26.013", "1.171", "Glycolytic enzyme. Can also function extracellularly as a cytokine, an angiogenic, or neurotrophic factor."], ["PDIA3", "Protein disulfide-isomerase A3", "26.026", "26.671", "1.093", "Protein folding, Molecular chaperone, secreted from platelets, Mediates platelet aggregation, haemostasis & thrombosis."], ["SAA1", "Serum amyloid A-1 protein", "22.977", "23.784", "2.692", "Major acute phase protein highly expressed in inflammation/tissue injury. Plays a role in HDL metabolism & cholesterol homeostasis. High levels associated with chronic inflammatory diseases including atherosclerosis."], ["SOD1", "Superoxide dismutase", "25.693", "27.181", "1.147", "One of two isozymes responsible for destroying free superoxide radicals in the body"], ["Proteins with decreased secretion in STEMI"], ["F5", "Coagulation factor V", "28.229", "27.63", "1.679", "Critical cofactor for the prothrombinase activity of Factor Xa that results in the activation of prothrombin to thrombin"], ["FN1", "Fibronectin", "29.416", "28.669", "2.768", "Component of subendothelial matrices and abundant in plasma. 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