Serum microRNA-592 serves as a novel potential biomarker for early diagnosis of colorectal cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:32724351", "kind": "science-record", "revision": 7, "content_hash": "sha256:0bbff717a7431863dd8cbe0d11bac17ee8d3ffe209ef0f93fcd8ec73a09a0afb", "created_at": "2026-11-01T15:41:06Z", "updated_at": "2026-11-01T22:01:57Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Serum microRNA-592 serves as a novel potential biomarker for early diagnosis of colorectal cancer.", "doi": "10.3892/ol.2020.11682", "pmid": "32724351", "authors": ["Zhenguo Pan", "Lin Miao"], "journal": "Oncology letters", "year": 2020, "abstract": "Colorectal cancer (CRC) is the second leading cause of cancer-associated mortality worldwide. Currently, available diagnostic biomarkers are neither sensitive nor specific. Thus, the present study aimed to identify novel circulating microRNAs (miRNAs) as biomarkers for the early diagnosis of CRC. All samples were provided by The Second Affiliated Hospital of Nanjing Medical University (Nanjing, China). Analysis of the GSE108153 and GSE55139 datasets, downloaded from the Gene Expression Omnibus (GEO) database was performed using the online tool, GEO2R. Reverse transcription-quantitative PCR was performed to determine miR-592 expression in CRC tissues, cells and serums of patients. Subsequently, the diagnostic value of serum miR-592 was assessed via receiver operating characteristic (ROC) curve analysis. Both the assessment of clinical samples and bioinformatics analysis demonstrated that miR-592 expression levels were significantly upregulated in the tissues and serum of patients with CRC, suggesting that elevated serum miR-592 may be tumor-derived. ROC analysis indicated that serum miR-592 levels may differentiate patients with early stage CRC and advanced adenoma from healthy individuals, with area under the curve values of 0.801 and 0.747, respectively. Taken together, the results of the present study suggest that serum miR-592 may be implicated as a potential biomarker for the early diagnosis of CRC.", "claims": [{"id": "claim-method", "statement": "The receiver operating characteristic (ROC) curve and area under the curve (AUC) were established to determine the diagnostic value of serum miR-592.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32724351", "locator": "sections[9] \"Statistical analysis\" paragraph 2", "quote": "The receiver operating characteristic (ROC) curve and area under the curve (AUC) were established to determine the diagnostic value of serum miR-592."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets generated and/or analyzed during the present study are available in the GEO repository, [http://www.ncbi.nlm.nih.gov/geo].", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32724351", "locator": "sections[18] \"Availability of data and materials\" paragraph 2", "quote": "The datasets generated and/or analyzed during the present study are available in the GEO repository, [http://www.ncbi.nlm.nih.gov/geo]."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:32724351"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE108153"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE108153"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:32724351", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.3892/ol.2020.11682", "sha256": "sha256:1d75c5d9b15bcdbf2d6b3e1003017aa4a1ce68eb7b063e4011ad4b2ebd249e57", "bytes": 21115, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.3892/ol.2020.11682", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC7377022", "source": "europe_pmc_fulltext", "coverage": "whole_body_without_references", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE108153", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE108153", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE108153", "version": null, "note": "The datasets generated and/or analyzed during the present study are available in the GEO repository, [http://www.ncbi.nlm.nih.gov/geo].", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The datasets generated and/or analyzed during the present study are available in the GEO repository, [http://www.ncbi.nlm.nih.gov/geo].", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM2891292", "GSM2891293", "GSM2891294", "GSM2891295", "GSM2891296", "GSM2891297", "GSM2891298", "GSM2891299", "GSM2891300", "GSM2891301", "GSM2891302", "GSM2891303", "GSM2891304", "GSM2891305", "GSM2891306", "GSM2891307", "GSM2891308", "GSM2891309", "GSM2891310", "GSM2891311", "GSM2891312", "GSM2891313", "GSM2891314", "GSM2891315", "GSM2891316", "GSM2891317", "GSM2891318", "GSM2891319", "GSM2891320", "GSM2891321", "GSM2891322", "GSM2891323", "GSM2891324", "GSM2891325", "GSM2891326", "GSM2891327", "GSM2891328", "GSM2891329", "GSM2891330", "GSM2891331", "GSM2891332", "GSM2891333"], "sample_count": 42, "group_sizes": null, "group_sizes_basis": null, "platform": "19730", "taxon": "Homo sapiens", "title": "MicroRNA Expression Analysis of Advanced Colorectal Cancer Reveals a microRNA Signature with Prognostic and Predictive Value", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE55139", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE55139", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE55139", "version": null, "note": "The datasets generated and/or analyzed during the present study are available in the GEO repository, [http://www.ncbi.nlm.nih.gov/geo].", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The datasets generated and/or analyzed during the present study are available in the GEO repository, [http://www.ncbi.nlm.nih.gov/geo].", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM1330397", "GSM1330398", "GSM1330399", "GSM1330400", "GSM1330401", "GSM1330402", "GSM1330403", "GSM1330404", "GSM1330405", "GSM1330406", "GSM1330407", "GSM1330408", "GSM1330409", "GSM1330410", "GSM1330411", "GSM1330412", "GSM1330413", "GSM1330414", "GSM1330415", "GSM1330416"], "sample_count": 20, "group_sizes": null, "group_sizes_basis": null, "platform": "14767", "taxon": "Homo sapiens", "title": "Identification of a novel biomarker serum miRNA from the patients with colorectal cancer (CRC).", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}], "gaps": ["asset:data:geo:GSE108153 not downloaded", "asset:data:geo:GSE108153 licence not read", "asset:data:geo:GSE55139 not downloaded", "asset:data:geo:GSE55139 licence not read"]}, "analysis": {"state": "partial", "methods": [{"id": "method-1", "family": "diagnostic_evaluation", "specific": "roc", "label": "Diagnostic test evaluation", "dsl_operations": ["confusion_counts", "roc_curve", "sensitivity_specificity", "solve_confusion_matrix", "classification_metrics", "published_precision_envelope"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["reference_standard_and_test"], "evidence": {"score": 0.7, "band": "medium", "quote": "ROC analysis"}}, {"id": "method-2", "family": "contingency_analysis", "specific": "chi_square", "label": "Categorical (contingency table) analysis", "dsl_operations": ["chi_square_test"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["categorical_cross_tabulation"], "evidence": {"score": 0.8, "band": "high", "quote": "χ2"}}, {"id": "method-3", "family": null, "specific": null, "label": "Receiver operating characteristic (ROC) curve and area under the curve (AUC) analysis", "dsl_operations": [], "missing_operations": [], "expressible": null, "parameters": {}, "data_shapes": [], "evidence": {"score": 1.0, "band": "verbatim", "quote": "The receiver operating characteristic (ROC) curve and area under the curve (AUC) were established to determine the diagnostic value of serum miR-592."}}], "targets": [], "gaps": ["method.reader_named_unmapped"]}, "capability": {"state": "complete", "requirements": [{"capability_id": "capability:computational_analysis", "label": "Computational analysis platform", "assay_families": ["meta_analysis", "machine_learning", "survival_analysis", "statistical_regression", "pca", "molecular_docking", "network_analysis", "differential_expression", "gwas"], "availability_state": "owned", "tier_unlocked": "R1", "candidate": "Automated Assays Laravel application and simulated adapters"}], "gaps": []}, "readiness": {"state": "complete", "tier": "R1", "blocking_axis": "none", "blocked_on": "nothing:replicable_now", "axes": {"method": {"tier": "R1", "expressible": true, "required_operations": ["confusion_counts", "roc_curve", "sensitivity_specificity", "solve_confusion_matrix", "classification_metrics", "published_precision_envelope"], "missing_operations": [], "basis": "The paper names the method it used and the corpus's method extractor maps it onto the DSL catalogue: roc."}, "capability": {"tier": "R1", "capability_id": "capability:computational_analysis", "availability_state": "owned", "basis": "The computational analysis is owned. The physical bench the paper used is not, so a physical validation cannot be run here."}, "data": {"tier": "R1", "reachable": true, "accessions": ["GSE108153", "GSE55139"], "resolved_accessions": ["GSE108153", "GSE55139"], "statements": ["The datasets generated and/or analyzed during the present study are available in the GEO repository, [http://www.ncbi.nlm.nih.gov/geo]."], "basis": "The paper names GSE108153, GSE55139 in Gene Expression Omnibus (GEO) and the accession resolves in the repository. No dataset was downloaded and no licence was read."}}, "can_run": {"dsl_operations": ["confusion_counts", "roc_curve", "sensitivity_specificity", "solve_confusion_matrix", "classification_metrics", "published_precision_envelope"], "assets": ["source:pmid:32724351", "data:geo:GSE108153"], "steps": ["run_analysis"]}, "must_build": {"dsl_operations": [], "converters": [], "readers": [], "capabilities": [], "data_gaps": ["data:geo:GSE108153"]}, "gaps": []}, "review": {"state": "partial", "machine_critique": {"kind": "extraction_review", "reader": {"model": "MiniMax-M3.1-Flash", "provider": "minimax", "provider_family": "minimax", "read_at": "2026-10-31T18:21:27Z", "independence": "single_reader"}, "evidence_check": {"method": "verbatim", "availability": "verbatim"}, "calls": 3, "usage": {"prompt_tokens": 16759, "completion_tokens": 1449, "cached_tokens": 784}}, "human_critique": null, "stages": [{"id": "extraction", "state": "complete", "reader": "MiniMax-M3.1-Flash", "provider": "minimax"}], "gaps": ["review.independent_reader", "review.machine_critique", "review.human_critique"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "pmid:32724351", "type": "paper", "label": "pmid:32724351"}, {"id": "source:pmid:32724351", "type": "manuscript", "label": "PMID 32724351 full text"}, {"id": "data:geo:GSE108153", "type": "dataset", "label": "GEO GSE108153"}], "activities": [{"id": "activity-reading-sci-2026-11-01", "type": "classification", "used": [{"id": "pmid:32724351", "type": "paper", "label": "pmid:32724351"}, {"id": "source:pmid:32724351", "type": "manuscript", "label": "PMID 32724351 full text"}, {"id": "data:geo:GSE108153", "type": "dataset", "label": "GEO GSE108153"}], "agent": "agent:corpus-assay-engine"}, {"id": "activity-read-32724351", "type": "extraction", "used": ["source:pmid:32724351"], "agent": "agent:minimax-reader"}, {"id": "activity-resolve-GSE108153", "type": "accession_resolution", "used": ["data:geo:GSE108153"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}, {"id": "agent:minimax-reader", "type": "software", "label": "MiniMax literature reader"}], "source_run_id": "reading-sci-2026-11-01", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "pmid:32724351", "parent_hash": "sha256:ddf0724ff1aca1917a8c879688c084fd0a96194c2a45029df2dab5257a1d36fd", "fork_of": null, "generation": 6}, "gaps": ["asset:data:geo:GSE108153 licence not read", "asset:data:geo:GSE108153 not downloaded", "asset:data:geo:GSE55139 licence not read", "asset:data:geo:GSE55139 not downloaded", "execution.not_run", "method.reader_named_unmapped", "review.human_critique", "review.independent_reader", "review.machine_critique"], "assays": [{"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["Institute of Biological Sciences. Cells were cultured in DMEM supplemented with 10% FBS (Thermo Fishe", "3C). miR-592 expression was also analyzed in cultured media of CRC cells (HCT116 and SW480 cells)."], "order_basis": "paper_text_order:3702", "id": "assay:1"}, {"role": "data_generation", "definition_id": "qpcr", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["mined across all CRC tissues and cell lines. RT-qPCR analysis demonstrated that miR-592 expressio", "n may be used to diagnose patients with CRC. RT-qPCR analysis indicated that serum miR-592 expres", "ed across all CRC tissues and cell lines. RT-qPCR analysis demonstrated that miR-592 expressio", "ay be used to diagnose patients with CRC. RT-qPCR analysis indicated that serum miR-592 expres"], "order_basis": "paper_text_order:6441", "id": "assay:2"}, {"role": "analysis", "definition_id": "diagnostic_evaluation", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["confusion_counts", "roc_curve", "sensitivity_specificity", "solve_confusion_matrix", "classification_metrics", "published_precision_envelope"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 0.7, "evidence": ["ROC analysis"], "order_basis": "consumes_earlier_output", "id": "assay:3"}]}