Integrative analysis of miRNA and mRNA sequencing data reveals potential regulatory mechanisms of ACE2 and TMPRSS2.

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In this work we searched for putative ACE2 and TMPRSS2 expression regulation networks mediated by various miRNA isoforms (isomiR) across different human organs using publicly available paired miRNA/mRNA-sequencing data from The Cancer Genome Atlas (TCGA) project. As a result, we identified several miRNA families targeting ACE2 and TMPRSS2 genes in multiple tissues. In particular, we found that lysine-specific demethylase 5B (JARID1B), encoded by the KDM5B gene, can indirectly affect ACE2 / TMPRSS2 expression by repressing transcription of hsa-let-7e / hsa-mir-125a and hsa-mir-141 / hsa-miR-200 miRNA families which are targeting these genes.", "claims": [{"id": "claim-method", "statement": "This observation indicates that the expression of KDM5B gene could be necessary for ACE2 expression (binomial test p = 2.2 × 10−7) in the majority of cells.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32726325", "locator": "sections[6] \"Expression of JARID1B is neccesary for ACE2 and TMPRSS2 expression in the majority of human cells\" paragraph 3", "quote": "This observation indicates that the expression of KDM5B gene could be necessary for ACE2 expression (binomial test p = 2.2 × 10−7) in the majority of cells."}], "state": "complete"}, {"id": "claim-data-access", "statement": "All relevant data are within the manuscript and its Supporting Information files. 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"PMC7390267", "section_index": 4, "section_title": "Landscape of interactions between isomiRs and ACE2 / TMPRSS2", "row_count": 11, "column_count": 2}, "table": {"label": "Table 1", "caption": "Intronic sense pre-miRNAs and their host genes.", "rows": [["pre-miRNA", "Host gene"], ["hsa-mir-335", "MEST"], ["hsa-mir-139", "PDE2A"], ["hsa-let-7e, hsa-mir-125a", "SPACA6"], ["hsa-mir-23b", "AOPEP"], ["hsa-let-7f-2, hsa-mir-98", "HUWE1"], ["hsa-mir-28", "LPP"], ["hsa-miR-9-1", "C1orf61"], ["hsa-mir-149", "GPC1"], ["hsa-mir-204", "TRPM3"], ["hsa-miR-664a", "RAB3GAP2"]], "row_count": 11, "column_count": 2, "numeric_cells": 11, "header_rows": 1, "locator": "section[4]/table-wrap[1]#pone.0235987.t001"}}, {"id": "asset:table:pmid:32726325:2", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:8471204e7e2582804dc706176cd50f9b1cf208112db72d6f62aeecf9bfeded41", "bytes": 1281, "access": "open", "state": "verified", "reader": 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