Time-Dependent miRNA Profile during Septic Acute Kidney Injury in Mice.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:32727087", "kind": "science-record", "revision": 8, "content_hash": "sha256:ce1b99b1a1e83ca20a896f681c0185c142ab8e76fc3e83f1a8b4791c01c2928b", "created_at": "2026-11-01T15:41:06Z", "updated_at": "2026-11-01T22:45:09Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Time-Dependent miRNA Profile during Septic Acute Kidney Injury in Mice.", "doi": "10.3390/ijms21155316", "pmid": "32727087", "authors": ["Pál Tod", "Beáta Róka", "Tamás Kaucsár", "Krisztina Szatmári", "Matej Vizovišek", "Robert Vidmar", "Marko Fonovič", "Gábor Szénási", "Péter Hamar"], "journal": "International journal of molecular sciences", "year": 2020, "abstract": "(1) Background: Lipopolysaccharide (LPS)-induced systemic inflammation is associated with septic acute kidney injury (AKI). We investigated the time-dependent miRNA expression changes in the kidney caused by LPS. (2) Methods: Male outbred NMRI mice were injected with LPS and sacrificed at 1.5 and 6 h (40 mg/kg i.p., early phase, EP) or at 24 and 48 h (10 mg/kg i.p., late phase, LP). The miRNA profile was established using miRCURY LNA™ microarray and confirmed with qPCR. Total renal proteome was analyzed by LC-MS/MS (ProteomeXchange: PXD014664). (3) Results: Septic AKI was confirmed by increases in plasma urea concentration and in renal TNF-α and IL-6 mRNA expression. Most miRNAs were altered at 6 and 24 h and declined by 48 h. In EP miR-762 was newly identified and validated and was the most elevated miRNA. The predicted target of miR-762, Ras related GTPase 1B (Sar1b) was downregulated. In LP miR-21a-5p was the most influenced miRNA followed by miR-451a, miR-144-3p, and miR-146a-5p. Among the potential protein targets of the most influenced miRNAs, only aquaporin-1, a target of miR-144-3p was downregulated at 24 h. (4) Conclusion: Besides already known miRNAs, septic AKI upregulated miR-762, which may regulate GTP signaling, and miR-144-3p and downregulated its target, aquaporin-1.", "claims": [{"id": "claim-method", "statement": "Statistical analysis was performed using one-way ANOVA. For multiple comparisons Dunnett’s post hoc test was used.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32727087", "locator": "sections[20] \"4.11. Statistics\" paragraph 2", "quote": "Statistical analysis was performed using one-way ANOVA. 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