Insights into Biomechanical and Proteomic Characteristics of Small Diameter Vascular Grafts Utilizing the Human Umbilical Artery.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:32785189", "kind": "science-record", "revision": 8, "content_hash": "sha256:4532ce6d6aabcdcb9c2d6a8f6dbce0007ff0bfb9875bc04388477cdc6f513a70", "created_at": "2026-11-01T15:41:07Z", "updated_at": "2026-11-01T22:45:11Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Insights into Biomechanical and Proteomic Characteristics of Small Diameter Vascular Grafts Utilizing the Human Umbilical Artery.", "doi": "10.3390/biomedicines8080280", "pmid": "32785189", "authors": ["Panagiotis Mallis", "Dimitrios P Sokolis", "Manousos Makridakis", "Jerome Zoidakis", "Athanasios D Velentzas", "Michalis Katsimpoulas", "Antonia Vlahou", "Alkiviadis Kostakis", "Catherine Stavropoulos-Giokas", "Efstathios Michalopoulos"], "journal": "Biomedicines", "year": 2020, "abstract": "The gold standard vascular substitutes, used in cardiovascular surgery, are the Dacron or expanded polytetrafluoroethylene (ePTFE)-derived grafts. However, major adverse reactions accompany their use. For this purpose, decellularized human umbilical arteries (hUAs) may be proven as a significant source for the development of small diameter conduits. The aim of this study was the evaluation of a decellularization protocol in hUAs. To study the effect of the decellularization to the hUAs, histological analysis was performed. Then, native and decellularized hUAs were biochemically and biomechanically evaluated. Finally, broad proteomic analysis was applied. Histological analysis revealed the successful decellularization of the hUAs. Furthermore, a great amount of DNA was removed from the decellularized hUAs. Biomechanical analysis revealed statistically significant differences in longitudinal direction only in maximum stress (p < 0.013) and strain (p < 0.001). On the contrary, all parameters tested for circumferential direction exhibited significant differences (p < 0.05). Proteomic analysis showed the preservation of the extracellular matrix and cytoskeletal proteins in both groups. Proteomic data are available via ProteomeXchange with identifier PXD020187. The above results indicated that hUAs were efficiently decellularized. The tissue function properties of these conduits were well retained, making them ideal candidates for the development of small diameter vascular grafts.", "claims": [{"id": "claim-method", "statement": "Collagen, sGAG, and DNA quantification data were compared using the Mann-Whitney test. Statistical significance was considered when the p-value was less than 0.05. Indicated values were the mean ± standard deviation. The unpaired Student’s t-test was used for comparisons of the biomechanical parameters between decellularized and native arteries, while comparisons between directions were assessed with the paired t-test. The Pearson correlation coefficient r was used for identifying associations of the biomechanical parameters with gestational age, infant’s weight, and arterial caliber, i.e., wall thickness and diameter. The effect of infant’s gender on the biomechanical properties was assessed with the unpaired t-test.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32785189", "locator": "sections[15] \"2.13. Statistical Analysis\" paragraph 2", "quote": "Collagen, sGAG, and DNA quantification data were compared using the Mann-Whitney test. Statistical significance was considered when the p-value was less than 0.05. Indicated values were the mean ± standard deviation. The unpaired Student’s t-test was used for comparisons of the biomechanical parameters between decellularized and native arteries, while comparisons between directions were assessed with the paired t-test. The Pearson correlation coefficient r was used for identifying associations of the biomechanical parameters with gestational age, infant’s weight, and arterial caliber, i.e., wall thickness and diameter. The effect of infant’s gender on the biomechanical properties was assessed with the unpaired t-test."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD020187.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32785189", "locator": "sections[24] \"Appendix A\" paragraph 2", "quote": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD020187."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:32785189"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:PXD020187"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "blocked", "blocked_on": "method:student_t", "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:PXD020187"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": ["step:run_analysis blocked on method:student_t"]}, "data": {"state": "partial", "assets": [{"id": "source:pmid:32785189", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.3390/biomedicines8080280", "sha256": "sha256:4e3628c0be69e7f69a6be9dae8030b034f33e7016db14cdedb1c6505f7a8c0c5", "bytes": 66784, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.3390/biomedicines8080280", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC7460081", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:PXD020187", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=PXD020187", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "PXD020187", "version": null, "note": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD020187.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD020187.", "access_statement_kind": "data_availability_statement", "sample_accessions": [], "sample_count": null, "group_sizes": null, "group_sizes_basis": null, "platform": null, "taxon": null, "title": "Proteomic evaluation of decellularized human umbilical artery", "resolution_checked_at": "2026-10-31T18:35:55Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "asset:table:pmid:32785189:1", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:490c26226d05d08a066962e528966878af09289d9552133402e4061f7f9129b8", "bytes": 2886, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[20]/table-wrap[1]#biomedicines-08-00280-t001", "version": null, "note": "List of representative identified proteins in native human umbilical arteries (hUAs).", "structure": {"pmcid": "PMC7460081", "section_index": 20, "section_title": "3.4. 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Statistical significance was considered when the p-value was less than 0.05. Indicated values were the mean ± standard deviation. The unpaired Student’s t-test was used for comparisons of the biomechanical parameters between decellularized and native arteries, while comparisons between directions were assessed with the paired t-test. The Pearson correlation coefficient r was used for identifying associations of the biomechanical parameters with gestational age, infant’s weight, and arterial caliber, i.e., wall thickness and diameter. 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Statistical significance was considered when the p-value was less than 0.05. Indicated values were the mean ± standard deviation. The unpaired Student’s t-test was used for comparisons of the biomechanical parameters between decellularized and native arteries, while comparisons between directions were assessed with the paired t-test. The Pearson correlation coefficient r was used for identifying associations of the biomechanical parameters with gestational age, infant’s weight, and arterial caliber, i.e., wall thickness and diameter. The effect of infant’s gender on the biomechanical properties was assessed with the unpaired t-test."], "order_basis": "analysis_only", "id": "assay:1"}]}