Pseudomonas aeruginosa Presents Multiple Vital Changes in Its Proteome in the Presence of 3-Hydroxyphenylacetic Acid, a Promising Antimicrobial Agent.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:32832748", "kind": "science-record", "revision": 6, "content_hash": "sha256:e8d3624b8da02c04115bc68c2ab21d245171a4bd5f1118c6d837903fd324a28a", "created_at": "2026-11-01T15:41:08Z", "updated_at": "2026-11-01T21:23:40Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Pseudomonas aeruginosa Presents Multiple Vital Changes in Its Proteome in the Presence of 3-Hydroxyphenylacetic Acid, a Promising Antimicrobial Agent.", "doi": "10.1021/acsomega.0c00703", "pmid": "32832748", "authors": ["Ozgun O Ozdemir", "Ferda Soyer"], "journal": "ACS omega", "year": 2020, "abstract": "Pseudomonas aeruginosa, a widely distributed opportunistic pathogen, is an important threat to human health for causing serious infections worldwide. Due to its antibiotic resistance and virulence factors, it is so difficult to combat this bacterium; thus, new antimicrobial agents are in search. 3-Hydroxyphenylacetic acid (3-HPAA), which is a phenolic acid mostly found in olive oil wastewater, can be a promising candidate with its dose-dependent antimicrobial properties. Elucidating the molecular mechanism of action is crucial for future examinations and the presentation of 3-HPAA as a new agent. In this study, the antimicrobial activity of 3-HPAA on P. aeruginosa and its action mechanism was investigated via shot-gun proteomics. The data, which are available via ProteomeXchange with identifier PXD016243, were examined by STRING analysis to determine the interaction networks of proteins. KEGG Pathway enrichment analysis via the DAVID bioinformatics tool was also performed to investigate the metabolic pathways that undetected and newly detected groups of the proteins. The results displayed remarkable changes after 3-HPAA exposure in the protein profile of P. aeruginosa related to DNA replication and repair, RNA modifications, ribosomes and proteins, cell envelope, oxidative stress, as well as nutrient availability. 3-HPAA showed its antimicrobial action on P. aeruginosa by affecting multiple bacterial processes; hence, it could be categorized as a multitarget antimicrobial agent.", "claims": [{"id": "claim-method", "statement": "The significant metabolic pathways of the genes of these proteins were elucidated by the KEGG pathway enrichment analysis using the DAVID bioinformatics database (version 6.8).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32832748", "locator": "sections[19] \"Data Analysis\" paragraph 2", "quote": "The significant metabolic pathways of the genes of these proteins were elucidated by the KEGG pathway enrichment analysis using the DAVID bioinformatics database (version 6.8)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD016243.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32832748", "locator": "sections[24] \"notes\" paragraph 1", "quote": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD016243."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:32832748"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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"ATP-dependent zinc metalloprotease FtsH", "ftsH PA4751", "cell division, response to antibiotic", "cell wall and membrane"], ["P50598", "Tol-Pal system protein TolQ", "tolQ PA0969", "cell cycle, cell division, bacteriocin transport", "cell wall and membrane"], ["P23189", "glutathione reductase", "gor PA2025", "cell redox homeostasis, response to oxygen radical", "redox and cell homeostasis"], ["Q9I6Z2", "alkyl hydroperoxide reductase", "ahpF PA0140", "response to reactive oxygen species", "redox and cell homeostasis"], ["P53652", "superoxide dismutase [Mn]", "sodA PA4468", "removal of superoxide radicals", "redox and cell homeostasis"], ["Q9I5R7", "S-adenosylmethionine decarboxylase proenzyme", "speD PA0654", "S-adenosylmethioninamine biosynthetic process, spermidine biosynthetic process", "redox and cell homeostasis"], ["Q9I5F9", "lon protease", "lon PA0779", "response to antibiotic, response to stress, nitric oxide metabolic process, peptidase activity", "redox and cell 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