Screening and Identification of Therapeutic Targets for Pulmonary Arterial Hypertension Through Microarray Technology.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:32849793", "kind": "science-record", "revision": 7, "content_hash": "sha256:834f5a5b59e9332c66c9cdce58bb519e67be824032458a94f9ecbd86933af121", "created_at": "2026-11-01T15:41:08Z", "updated_at": "2026-11-01T22:02:05Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Screening and Identification of Therapeutic Targets for Pulmonary Arterial Hypertension Through Microarray Technology.", "doi": "10.3389/fgene.2020.00782", "pmid": "32849793", "authors": ["Qing Li", "LingBing Meng", "DePing Liu"], "journal": "Frontiers in genetics", "year": 2020, "abstract": "Pulmonary arterial hypertension (PAH) is a rare but fatal disease characterized by vascular cell proliferation; the pathogenesis of PAH has yet to be fully elucidated. Publicly available genetic data were downloaded from the Gene Expression Omnibus (GEO) database, and gene set enrichment analysis (GSEA) was used to determine significant differences in gene expression between tissues with PAH and healthy lung tissues. Differentially expressed genes (DEGs) were identified using the online tool, GEO2R, and functional annotation of DEGs was performed using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Next, the construction and module analysis of the protein-protein interaction (PPI) network and verification of the expression level of hub genes was performed. Finally, prediction and enrichment analysis of microRNAs associated with the hub genes was carried out. A total of 110 DEGs were detected by screening PAH and healthy lung samples. The expression of nine genes [polo-like kinase 4 (PLK4), centromere protein U, kinesin family member 20B, structural maintenance of chromosome 2 (SMC2), abnormal spindle microtubule assembly, Fanconi Anemia complementation group I, kinesin family member 18A, spindle apparatus coiled-coil protein 1, and MIS18 binding protein 1] was elevated in PAH; this was statistically significant compared with their expression in healthy lung tissue, and they were identified as hub genes. GO and KEGG analysis showed that the variations in DEGs were abundant in DNA-templated transcription, sister chromatid cohesion, mitotic nuclear division, cell proliferation, and regulation of the actin cytoskeleton. In conclusion, this study has successfully identified hub genes and key pathways of PAH, with a total of 110 DEGs and nine hub genes related to PAH, especially the PLK4 and SMC2 genes, thus providing important clues for the in-depth understanding of the molecular mechanism of PAH and providing potential therapeutic targets.", "claims": [{"id": "claim-method", "statement": "The interactive web tool, GEO2R, was used to screen and identify differentially expressed genes (DEGs) by comparing samples from the GEO series (Barrett et al., 2013).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32849793", "locator": "sections[1] \"Introduction\" paragraph 5", "quote": "The interactive web tool, GEO2R, was used to screen and identify differentially expressed genes (DEGs) by comparing samples from the GEO series (Barrett et al., 2013)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets generated for this study can be found in the Gene Expression Omnibus (GEO, www.ncbi.nlm.nih.gov/geo), GSE15197, GSE53408, GSE113439, and GSE117261.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32849793", "locator": "sections[21] \"Data Availability Statement\" paragraph 2", "quote": "The datasets generated for this study can be found in the Gene Expression Omnibus (GEO, www.ncbi.nlm.nih.gov/geo), GSE15197, GSE53408, GSE113439, and GSE117261."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:32849793"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE113439"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE113439"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:32849793", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.3389/fgene.2020.00782", "sha256": 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"state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[18]/table-wrap[1]#T4", "version": null, "note": "Chemicals that may affect the expression of hub genes in pulmonary arterial hypertension.", "structure": {"pmcid": "PMC7396553", "section_index": 10, "section_title": "Identification of Chemicals Associated With Hub Genes", "row_count": 10, "column_count": 6}, "table": {"label": "TABLE 4", "caption": "Chemicals that may affect the expression of hub genes in pulmonary arterial hypertension.", "rows": [["Num.", "Gene ID", "Gene symbol", "Gene name", "Inference network", "Inference score"], ["1", "9585", "KIF20B", "Kinesin Family Member 20B", "Copper sulfate, epigallocatechin gallate, oxygen, quercetin, valproic acid", "12.78"], ["2", "10592", "SMC2", "Structural maintenance Of chromosomes 2", "4,4′-diaminodiphenylmethane, epigallocatechin gallate, quercetin, resveratrol, valproic acid", "12.74"], ["3", "10733", "PLK4", "Polo like kinase 4", 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ADAMTS9, ASPM, ATP6V1C1, ATRX, BCLAF1, BLZF1, BZW1, CCP110, CENPU, CMAHP, DDX21, DOCK10, EEA1, FANCI, FGF7, FRK, GAPT, GCC2, HOOK3, IARS, ITPR2, JMJD1C, KIAA0368, KIF18A, KIF20B, KRR1, MAPK6, MET, MIS18BP1, NAA15, PBRM1, PDGFD, PHF20L1, PIK3C2A, PLK4, POLK, PPP1R12A, PYROXD1, RANBP6, RIOK2, RNF6, S100A3, SAMD9, SCN4B, SERPINB2, SLC6A14, SLC7A11, SLK, SLTM, SMC2, SMC5, SOCS4, SOSTDC1, SPDL1, SRFBP1, STAG2, STEAP1, SUCLA2, THOC2, TXNRD1, USP34, ZC3H15, ZNF267, ZNF845"], ["Down-regulated DEGs", "ALAS2, ANKRD50, ASCC3, BOD1L1, CA1, CCDC186, DDX52, DST, EHF, EPHA4, ESF1, FMO5, FNBP1L, HIST2H2AC, HLTF, HOOK1, HSP90AA1, IGF1, IQGAP2, ITGA2, KIAA1109, LBH, MACC1, MYCBP2, MYO5A, N4BP2, NCL, NIPBL, NSF, OSBPL8, RFC1, RSPO3, RSRC1, SECISBP2L, SUPT16H, TFEC, TTN, TXLNG, UBXN4, USP15, VEPH1, ZC3H13, ZFX, ZNF148, ZNF292"]], "row_count": 3, "column_count": 2, "numeric_cells": 2, "header_rows": 1, "locator": "section[13]/table-wrap[2]#T2"}}, {"id": "asset:table:pmid:32849793:5", "role": "table", 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