Short-Term Mild Temperature-Stress-Induced Alterations in the C. elegans Phosphoproteome.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:32899194", "kind": "science-record", "revision": 7, "content_hash": "sha256:250748ae3ce22867417d851a6dbaed5c1a13162f4e5184b800ac0ee894d4ea94", "created_at": "2026-11-01T15:41:09Z", "updated_at": "2026-11-01T22:02:08Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Short-Term Mild Temperature-Stress-Induced Alterations in the C. elegans Phosphoproteome.", "doi": "10.3390/ijms21176409", "pmid": "32899194", "authors": ["Jichang Huang", "Zhen Wu", "Xumin Zhang"], "journal": "International journal of molecular sciences", "year": 2020, "abstract": "Exposure to mild early-life stresses can slow down aging, and protein phosphorylation might be an essential regulator in this process. However, the mechanisms of phosphorylation-based signaling networks during mild early-life stress remain elusive. Herein, we systematically analyzed the phosphoproteomes of Caenorhabditis elegans, which were treated with three mild temperatures (15 °C, 20 °C, and 25 °C) in two different short-term groups (10 min and 60 min). By utilizing an iTRAQ-based quantitative phosphoproteomic approach, 18,187 phosphosites from 3330 phosphoproteins were detected in this study. Volcano plots illustrated that the phosphorylation abundance of 374 proteins and 347 proteins, were significantly changed at 15 °C and 25 °C, respectively. Gene ontology, KEGG pathway and protein-protein interaction network analyses revealed that these phosphoproteins were primarily associated with metabolism, translation, development, and lifespan determination. A motif analysis of kinase substrates suggested that MAPK, CK, and CAMK were most likely involved in the adaption processes. Moreover, 16 and 14 aging-regulated proteins were found to undergo phosphorylation modifications under the mild stresses of 15 °C and 25 °C, respectively, indicating that these proteins might be important for maintaining long-term health. Further lifespan experiments confirmed that the candidate phosphoproteins, e.g., EGL-27 and XNP-1 modulated longevity at 15 °C, 20 °C, and 25 °C, and they showed increased tolerance to thermal and oxidative stresses. In conclusion, our findings offered data that supports understanding of the phosphorylation mechanisms involved in mild early-life stresses in C. elegans. Data are available via ProteomeXchange with identifier PXD021081.", "claims": [{"id": "claim-method", "statement": "The log-rank (Kaplan–Meier) test was used to calculate p-values.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32899194", "locator": "sections[18] \"4.7. 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