An Organometallic Gold(I) Bis-N-Heterocyclic Carbene Complex with Multimodal Activity in Ovarian Cancer Cells.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:32902006", "kind": "science-record", "revision": 6, "content_hash": "sha256:049ab2affa93994c168fb27aae12a5b8e2953b1a2fc4082d323c71e9e4f4b7e9", "created_at": "2026-11-01T15:41:09Z", "updated_at": "2026-11-01T21:23:45Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "An Organometallic Gold(I) Bis-N-Heterocyclic Carbene Complex with Multimodal Activity in Ovarian Cancer Cells.", "doi": "10.1002/chem.202003495", "pmid": "32902006", "authors": ["Samuel M Meier-Menches", "Benjamin Neuditschko", "Katja Zappe", "Martin Schaier", "Marlene C Gerner", "Klaus G Schmetterer", "Giorgia Del Favero", "Riccardo Bonsignore", "Margit Cichna-Markl", "Gunda Koellensperger", "Angela Casini", "Christopher Gerner"], "journal": "Chemistry (Weinheim an der Bergstrasse, Germany)", "year": 2020, "abstract": "The organometallic AuI bis-N-heterocyclic carbene complex [Au(9-methylcaffeine-8-ylidene)2 ]+ (AuTMX2 ) was previously shown to selectively and potently stabilise telomeric DNA G-quadruplex (G4) structures. This study sheds light on the molecular reactivity and mode of action of AuTMX2 in the cellular context using mass spectrometry-based methods, including shotgun proteomics in A2780 ovarian cancer cells. In contrast to other metal-based anticancer agents, this organogold compound is less prone to form coordinative bonds with biological nucleophiles and is expected to exert its drug effects mainly by non-covalent interactions. Global protein expression changes of treated cancer cells revealed a multimodal mode of action of AuTMX2 by alterations in the nucleolus, telomeres, actin stress-fibres and stress-responses, which were further supported by pharmacological assays, fluorescence microscopy and cellular accumulation experiments. 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