Mutations in the notch signalling pathway are associated with enhanced anti-tumour immunity in colorectal cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:32924269", "kind": "science-record", "revision": 7, "content_hash": "sha256:9d8247fd43d900fc60a2d72b3ed9a8d6e045ec1cc476a5695043244dc6826574", "created_at": "2026-11-01T15:41:10Z", "updated_at": "2026-11-01T22:02:11Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Mutations in the notch signalling pathway are associated with enhanced anti-tumour immunity in colorectal cancer.", "doi": "10.1111/jcmm.15867", "pmid": "32924269", "authors": ["Fei Wang", "Jie Long", "Liang Li", "Zhi-Bin Zhao", "Fang Wei", "Yuan Yao", "Wen-Jing Qiu", "Zi-Xin Wu", "Qing-Qing Luo", "Wei Liu", "Yi-Bo Quan", "Zhe-Xiong Lian", "Jie Cao"], "journal": "Journal of cellular and molecular medicine", "year": 2020, "abstract": "The Notch signalling pathway is involved in the development of several cancers, including colorectal cancer (CRC). However, whether mutations in this pathway could alter the CRC immunophenotype remains unknown. Here, we investigated the relationship between Notch signalling pathway mutations and the tumour immune microenvironment by analysing gene expression data from the GSE108989 single T cell RNA sequencing data set and The Cancer Genome Atlas (TCGA) data set. We found that Notch signalling pathway mutations were associated with an increased number of tumour-specific CD8+ T cells and decreased number of inhibitory regulatory T (Treg) cells, representing an enhanced anti-tumour response in the GSE108989 data set. In TCGA data set, we also found that Notch signalling pathway mutations were associated with enrichment of genes associated with immune activation pathways and higher expressions of PDCD1, GZMB and PRF1. Although Notch signalling pathway mutations did not affect the overall survival and disease-free survival of CRC patients, they were associated with earlier disease stages and lower rates of metastasis. These results demonstrated that Notch signalling pathway mutations can enhance anti-tumour immunity in CRC, as validated by the two data sets, suggesting that they may be promising biomarkers for immune checkpoint blockade therapies for CRC patients.", "claims": [{"id": "claim-method", "statement": "The P values were calculated by the Mann‐Whitney U test and used to determine the statistical significance in IBM SPSS statistics 21.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32924269", "locator": "sections[5] \"CRC TCGA data set analysis\" paragraph 2", "quote": "The P values were calculated by the Mann‐Whitney U test and used to determine the statistical significance in IBM SPSS statistics 21."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The single T cell sequencing raw data are available as GEO accession GSE108989: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc = GSE108989. 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