Enhanced fatty acid oxidation mediated by CPT1C promotes gastric cancer progression.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:32953153", "kind": "science-record", "revision": 3, "content_hash": "sha256:47ecdf8af66394ca1141fd496afa418d9fd12902be95ac622859ecf7ef081c67", "created_at": "2026-11-01T17:34:06Z", "updated_at": "2026-11-01T21:23:49Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Enhanced fatty acid oxidation mediated by CPT1C promotes gastric cancer progression.", "doi": "10.21037/jgo-20-157", "pmid": "32953153", "authors": ["Tianyi Chen", "Guiyang Wu", "Hai Hu", "Chongshan Wu"], "journal": "Journal of gastrointestinal oncology", "year": 2020, "abstract": "BACKGROUND: Carnitine palmitoyltransferase 1C (CPT1C) is a critical enzyme that catalyzes carnitinylation of fatty acids for transport into mitochondria for β-oxidation. No previous studies have been conducted to explore the prognostic and oncogenic role of CPT1C in gastric cancer (GC).\nMETHODS: Public RNA-sequencing data and micro-array data were extracted from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases respectively. Survival analysis was performed in TCGA and GSE62254 cohorts. RT-qPCR and Western blot analyses were used to determine genes expression in GC cells. Fatty acid oxidation (FAO) assay kit was used to examine cell FAO rate. The cell proliferation ability and cell cycle were tested by using CCK-8 and cell cycle assay kits.\nRESULTS: In the both TCGA and GSE62254 cohorts, high expression of CPT1C was significantly associated with poor overall (OS) (P<0.001) and disease free survival (DFS) of GC patients (P<0.001). Silence of CPT1C significantly inhibited cell FAO rate, suppressed cell proliferation and induced cell cycle arrest, while enforced CPT1C expression had the opposite effects. However, etomoxir treatment completely restricted the increase of FAO rate, cell viability and the phase of DNA synthesis caused by enhanced CPT1C expression. Of note, CPT1C expression was transcriptionally activated by hypoxia inducible factor-1α.\nCONCLUSIONS: High expression of CPT1C induced by hypoxia was closely associated with poor prognosis and can promote proliferation of GC cells.", "claims": [{"id": "claim-method", "statement": "Survival analysis was performed in TCGA and GSE62254 cohorts.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32953153", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "Survival analysis was performed in TCGA and GSE62254 cohorts."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Public RNA-sequencing data and micro-array data were extracted from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases respectively.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32953153", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "Public RNA-sequencing data and micro-array data were extracted from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases respectively."}], "state": "complete"}, {"id": "claim-target-result", "statement": "High expression of CPT1C induced by hypoxia was closely associated with poor prognosis and can promote proliferation of GC cells.", "kind": "quantitative", "target": {"value": 0.001, "unit": "p-value", "tolerance": null, "source": "In the both TCGA and GSE62254 cohorts, high expression of CPT1C was significantly associated with poor overall (OS) (P<0.001) and disease free survival (DFS) of GC patients (P<0.001)."}, "evidence": [{"asset": "source:pmid:32953153", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "In the both TCGA and GSE62254 cohorts, high expression of CPT1C was significantly associated with poor overall (OS) (P<0.001) and disease free survival (DFS) of GC patients (P<0.001)."}, {"asset": "source:pmid:32953153", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "High expression of CPT1C induced by hypoxia was closely associated with poor prognosis and can promote proliferation of GC cells."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "High expression of CPT1C induced by hypoxia was closely associated with poor prognosis and can promote proliferation of GC cells.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:32953153"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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