RNAi Screening Identifies that TEX10 Promotes the Proliferation of Colorectal Cancer Cells by Increasing NF-κB Activation.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:32995120", "kind": "science-record", "revision": 7, "content_hash": "sha256:af37ad22f45605efb68656253e3aca1ff4ecd9206acb11afb08a2846f73fbca3", "created_at": "2026-11-01T15:41:11Z", "updated_at": "2026-11-01T22:02:16Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "RNAi Screening Identifies that TEX10 Promotes the Proliferation of Colorectal Cancer Cells by Increasing NF-κB Activation.", "doi": "10.1002/advs.202000593", "pmid": "32995120", "authors": ["Ziyang Wang", "Chunjie Sheng", "Guangyan Kan", "Chen Yao", "Rong Geng", "Shuai Chen"], "journal": "Advanced science (Weinheim, Baden-Wurttemberg, Germany)", "year": 2020, "abstract": "Colorectal cancer (CRC) has become a predominant cancer worldwide. To understand the process of carcinogenesis, a short hairpin RNA library screening is employed to search for candidate genes that promote proliferation in the CRC cell line HT29. The candidate genes overlap with differentially expressed genes in 32 CRC tumor tissues in the GEO dataset GSE8671. The seventh-ranked testis expressed 10 (TEX10) is upregulated in CRC and its knockdown decreases cell proliferation. The TEX10 high-expression group exhibits worse overall survival (P = 0.003) and progression-free survival (P = 0.001) than the TEX10 low-expression group. TEX10 depletion decreases the growth of CRC cells in vitro and in vivo. Gene set enrichment analysis indicates that the nuclear factor-kappa B pathway is significantly enriched in the genes downregulated by TEX10 knockdown. Mechanistically, TEX10 interacts with RELA and increases its nuclear localization. TEX10 promotes RELA occupancy at gene promoters and regulates the expression of a subset of RELA-targeted genes, including TNFAIP8, SAT1, and IL6ST. Taken together, this study identifies that TEX10 promotes the proliferation of CRC cells in an RELA-dependent manner. In addition, high TEX10 expression is associated with poor prognosis in CRC patients.", "claims": [{"id": "claim-method", "statement": "The Cox proportional hazards model was used for analyzing associations of variables with survival.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32995120", "locator": "sections[31] \"Statistical Analysis\" paragraph 2", "quote": "The Cox proportional hazards model was used for analyzing associations of variables with survival."}], "state": "complete"}, {"id": "claim-data-access", "statement": "RNAseq data have been submitted to the GEO with accession number GSE147334. Raw data of this study have been deposited to the Research Data Deposit database (http://www.researchdata.org.cn), with the Approval Number as RDDB2020000881.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:32995120", "locator": "sections[32] \"Data Availability\" paragraph 2", "quote": "RNAseq data have been submitted to the GEO with accession number GSE147334. Raw data of this study have been deposited to the Research Data Deposit database (http://www.researchdata.org.cn), with the Approval Number as RDDB2020000881."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:32995120"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE12945"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE12945"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:32995120", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1002/advs.202000593", "sha256": "sha256:53c9aedfa407615442d0ee56b49a7d6f9380078b215d5f8bde654291c38f763e", "bytes": 43482, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1002/advs.202000593", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC7507032", "source": "europe_pmc_fulltext", "coverage": "whole_body_without_references", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE12945", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE12945", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE12945", "version": null, "note": "RNAseq data have been submitted to the GEO with accession number GSE147334. Raw data of this study have been deposited to the Research Data Deposit database (http://www.researchdata.org.cn), with the Approval Number as RDDB2020000881.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "RNAseq data have been submitted to the GEO with accession number GSE147334. Raw data of this study have been deposited to the Research Data Deposit database (http://www.researchdata.org.cn), with the Approval Number as RDDB2020000881.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM324715", "GSM324716", "GSM324717", "GSM324718", "GSM324719", "GSM324720", "GSM324721", "GSM324722", "GSM324723", "GSM324724", "GSM324725", "GSM324726", "GSM324727", "GSM324728", "GSM324729", "GSM324730", "GSM324731", "GSM324732", "GSM324733", "GSM324734", "GSM324735", "GSM324736", "GSM324737", "GSM324738", "GSM324739", "GSM324740", "GSM324741", "GSM324742", "GSM324743", "GSM324744", "GSM324745", "GSM324746", "GSM324747", "GSM324748", "GSM324749", "GSM324750", "GSM324751", "GSM324752", "GSM324753", "GSM324754", "GSM324755", "GSM324756", "GSM324757", "GSM324758", "GSM324759", "GSM324760", "GSM324761", "GSM324762", "GSM324763", "GSM324764", "GSM324765", "GSM324766", "GSM324767", "GSM324768", "GSM324769", "GSM324770", "GSM324771", "GSM324772", "GSM324773", "GSM324774", "GSM324775", "GSM324776"], "sample_count": 62, "group_sizes": null, "group_sizes_basis": null, "platform": "96", "taxon": "Homo sapiens", "title": "Expression data from colorectal cancers", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE147334", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE147334", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE147334", "version": null, "note": "RNAseq data have been submitted to the GEO with accession number GSE147334. Raw data of this study have been deposited to the Research Data Deposit database (http://www.researchdata.org.cn), with the Approval Number as RDDB2020000881.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "RNAseq data have been submitted to the GEO with accession number GSE147334. Raw data of this study have been deposited to the Research Data Deposit database (http://www.researchdata.org.cn), with the Approval Number as RDDB2020000881.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM4426221", "GSM4426222", "GSM4426223", "GSM4426224", "GSM4426225", "GSM4426226", "GSM4426227", "GSM4426228", "GSM4426229", "GSM4426230"], "sample_count": 10, "group_sizes": null, "group_sizes_basis": null, "platform": "11154", "taxon": "Homo sapiens", "title": "RNAi Screening Identifies that TEX10 Promotes the Proliferation of Colorectal Cancer Cells by Increasing NF-κB Activation", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE17536", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE17536", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE17536", "version": null, "note": "RNAseq data have been submitted to the GEO with accession number GSE147334. Raw data of this study have been deposited to the Research Data Deposit database (http://www.researchdata.org.cn), with the Approval Number as RDDB2020000881.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "RNAseq data have been submitted to the GEO with accession number GSE147334. Raw data of this study have been deposited to the Research Data Deposit database (http://www.researchdata.org.cn), with the Approval Number as RDDB2020000881.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM437093", "GSM437094", "GSM437095", "GSM437096", "GSM437097", "GSM437098", "GSM437099", "GSM437100", "GSM437101", "GSM437102", "GSM437103", "GSM437104", "GSM437105", "GSM437106", "GSM437107", "GSM437108", "GSM437109", "GSM437110", "GSM437111", "GSM437112", "GSM437113", "GSM437114", "GSM437115", "GSM437116", "GSM437117", "GSM437118", "GSM437119", "GSM437120", "GSM437121", "GSM437122", "GSM437123", "GSM437124", "GSM437125", "GSM437126", "GSM437127", "GSM437128", "GSM437129", "GSM437130", "GSM437131", "GSM437132", "GSM437133", "GSM437134", "GSM437135", "GSM437136", "GSM437137", "GSM437138", "GSM437139", "GSM437140", "GSM437141", "GSM437142", "GSM437143", "GSM437144", "GSM437145", "GSM437146", "GSM437147", "GSM437148", "GSM437149", "GSM437150", "GSM437151", "GSM437152", "GSM437153", "GSM437154", "GSM437155", "GSM437156", "GSM437157", "GSM437158", "GSM437159", "GSM437160", "GSM437161", "GSM437162", "GSM437163", "GSM437164", "GSM437165", "GSM437166", "GSM437167", "GSM437168", "GSM437169", "GSM437170", "GSM437171", "GSM437172", "GSM437173", "GSM437174", "GSM437175", "GSM437176", "GSM437177", "GSM437178", "GSM437179", "GSM437180", "GSM437181", "GSM437182", "GSM437183", "GSM437184", "GSM437185", "GSM437186", "GSM437187", "GSM437188", "GSM437189", "GSM437190", "GSM437191", "GSM437192", "GSM437193", "GSM437194", "GSM437195", "GSM437196", "GSM437197", "GSM437198", "GSM437199", "GSM437200", "GSM437201", "GSM437202", "GSM437203", "GSM437204", "GSM437205", "GSM437206", "GSM437207", "GSM437208", "GSM437209", "GSM437210", "GSM437211", "GSM437212", "GSM437213", "GSM437214", "GSM437215", "GSM437216", "GSM437217", "GSM437218", "GSM437219", "GSM437220", "GSM437221", "GSM437222", "GSM437223", "GSM437224", "GSM437225", "GSM437226", "GSM437227", "GSM437228", "GSM437229", "GSM437230", "GSM437231", "GSM437232", "GSM437233", "GSM437234", "GSM437235", "GSM437236", "GSM437237", "GSM437238", "GSM437239", "GSM437240", "GSM437241", "GSM437242", "GSM437243", "GSM437244", "GSM437245", "GSM437246", "GSM437247", "GSM437248", "GSM437249", "GSM437250", "GSM437251", "GSM437252", "GSM437253", "GSM437254", "GSM437255", "GSM437256", "GSM437257", "GSM437258", "GSM437259", "GSM437260", "GSM437261", "GSM437262", "GSM437263", "GSM437264", "GSM437265", "GSM437266", "GSM437267", "GSM437268", "GSM437269"], "sample_count": 177, "group_sizes": null, "group_sizes_basis": null, "platform": "570", "taxon": "Homo sapiens", "title": "Metastasis Gene Expression Profile Predicts Recurrence and Death in Colon Cancer Patients (Moffitt Samples)", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE17537", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE17537", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE17537", "version": null, "note": "RNAseq data have been submitted to the GEO with accession number GSE147334. Raw data of this study have been deposited to the Research Data Deposit database (http://www.researchdata.org.cn), with the Approval Number as RDDB2020000881.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "RNAseq data have been submitted to the GEO with accession number GSE147334. Raw data of this study have been deposited to the Research Data Deposit database (http://www.researchdata.org.cn), with the Approval Number as RDDB2020000881.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM437270", "GSM437271", "GSM437272", "GSM437273", "GSM437274", "GSM437275", "GSM437276", "GSM437277", "GSM437278", "GSM437279", "GSM437280", "GSM437281", "GSM437282", "GSM437283", "GSM437284", "GSM437285", "GSM437286", "GSM437287", "GSM437288", "GSM437289", "GSM437290", "GSM437291", "GSM437292", "GSM437293", "GSM437294", "GSM437295", "GSM437296", "GSM437297", "GSM437298", "GSM437299", "GSM437300", "GSM437301", "GSM437302", "GSM437303", "GSM437304", "GSM437305", "GSM437306", "GSM437307", "GSM437308", "GSM437309", "GSM437310", "GSM437311", "GSM437312", "GSM437313", "GSM437314", "GSM437315", "GSM437316", "GSM437317", "GSM437318", "GSM437319", "GSM437320", "GSM437321", "GSM437322", "GSM437323", "GSM437324"], "sample_count": 55, "group_sizes": null, "group_sizes_basis": null, "platform": "570", "taxon": "Homo sapiens", "title": "Metastasis Gene Expression Profile Predicts Recurrence and Death in Colon Cancer Patients (VMC Samples)", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE8671", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE8671", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE8671", "version": null, "note": "RNAseq data have been submitted to the GEO with accession number GSE147334. Raw data of this study have been deposited to the Research Data Deposit database (http://www.researchdata.org.cn), with the Approval Number as RDDB2020000881.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "RNAseq data have been submitted to the GEO with accession number GSE147334. Raw data of this study have been deposited to the Research Data Deposit database (http://www.researchdata.org.cn), with the Approval Number as RDDB2020000881.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM215051", "GSM215052", "GSM215053", "GSM215054", "GSM215055", "GSM215056", "GSM215057", "GSM215058", "GSM215059", "GSM215060", "GSM215061", "GSM215062", "GSM215063", "GSM215064", "GSM215065", "GSM215066", "GSM215067", "GSM215068", "GSM215069", "GSM215070", "GSM215071", "GSM215072", "GSM215073", "GSM215074", "GSM215075", "GSM215076", "GSM215077", "GSM215078", "GSM215079", "GSM215080", "GSM215081", "GSM215082", "GSM215083", "GSM215084", "GSM215085", "GSM215086", "GSM215087", "GSM215088", "GSM215089", "GSM215090", "GSM215091", "GSM215092", "GSM215093", "GSM215094", "GSM215095", "GSM215096", "GSM215097", "GSM215098", "GSM215099", "GSM215100", "GSM215101", "GSM215102", "GSM215103", "GSM215104", "GSM215105", "GSM215106", "GSM215107", "GSM215108", "GSM215109", "GSM215110", "GSM215111", "GSM215112", "GSM215113", "GSM215114"], "sample_count": 64, "group_sizes": null, "group_sizes_basis": null, "platform": "570", "taxon": "Homo sapiens", "title": "Transcriptome profile of human colorectal adenomas.", "resolution_checked_at": "2026-11-01T15:39:55Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}], "gaps": ["asset:data:geo:GSE12945 not downloaded", "asset:data:geo:GSE12945 licence not read", "asset:data:geo:GSE147334 not downloaded", "asset:data:geo:GSE147334 licence not read", "asset:data:geo:GSE17536 not downloaded", "asset:data:geo:GSE17536 licence not read", "asset:data:geo:GSE17537 not downloaded", "asset:data:geo:GSE17537 licence not read", "asset:data:geo:GSE8671 not downloaded", "asset:data:geo:GSE8671 licence not read"]}, "analysis": {"state": "partial", "methods": [{"id": "method-1", "family": "survival_analysis", "specific": "cox_proportional_hazards", "label": "Survival analysis", "dsl_operations": ["cox_ph"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["time_to_event_with_censoring"], "evidence": {"score": 1.0, "band": "verbatim", "quote": "The Cox proportional hazards model was used for analyzing associations of variables with survival."}}, {"id": "method-2", "family": "group_comparison", "specific": "anova", "label": "Group comparison", "dsl_operations": ["group_summary", "welch_t", "bootstrap_mean_difference", "group_comparison_anova"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["two_or_more_groups_continuous"], "evidence": {"score": 0.8, "band": "high", "quote": "analysis of variance"}}], "targets": [], "gaps": ["method.reader_differs_from_extractor"]}, "capability": {"state": "complete", "requirements": [{"capability_id": "capability:computational_analysis", "label": "Computational analysis platform", "assay_families": ["meta_analysis", "machine_learning", "survival_analysis", "statistical_regression", "pca", "molecular_docking", "network_analysis", "differential_expression", "gwas"], "availability_state": "owned", "tier_unlocked": "R1", "candidate": "Automated Assays Laravel application and simulated adapters"}], "gaps": []}, "readiness": {"state": "complete", "tier": "R1", "blocking_axis": "none", "blocked_on": "nothing:replicable_now", "axes": {"method": {"tier": "R1", "expressible": true, "required_operations": ["cox_ph"], "missing_operations": [], "basis": "The paper names the method it used and the corpus's method extractor maps it onto the DSL catalogue: cox_proportional_hazards."}, "capability": {"tier": "R1", "capability_id": "capability:computational_analysis", "availability_state": "owned", "basis": "The computational analysis is owned. The physical bench the paper used is not, so a physical validation cannot be run here."}, "data": {"tier": "R1", "reachable": true, "accessions": ["GSE8671", "GSE12945", "GSE17536", "GSE17537", "GSE147334"], "resolved_accessions": ["GSE8671", "GSE12945", "GSE17536", "GSE17537", "GSE147334"], "statements": ["RNAseq data have been submitted to the GEO with accession number GSE147334. Raw data of this study have been deposited to the Research Data Deposit database (http://www.researchdata.org.cn), with the Approval Number as RDDB2020000881."], "basis": "The paper names GSE8671, GSE12945, GSE17536, GSE17537, GSE147334 in Gene Expression Omnibus (GEO) and the accession resolves in the repository. No dataset was downloaded and no licence was read."}}, "can_run": {"dsl_operations": ["cox_ph"], "assets": ["source:pmid:32995120", "data:geo:GSE12945"], "steps": ["run_analysis"]}, "must_build": {"dsl_operations": [], "converters": [], "readers": [], "capabilities": [], "data_gaps": ["data:geo:GSE12945"]}, "gaps": []}, "review": {"state": "partial", "machine_critique": {"kind": "extraction_review", "reader": {"model": "MiniMax-M3.1-Flash", "provider": "minimax", "provider_family": "minimax", "read_at": "2026-10-31T18:22:57Z", "independence": "single_reader"}, "evidence_check": {"method": "verbatim", "availability": "verbatim"}, "calls": 3, "usage": {"prompt_tokens": 34620, "completion_tokens": 1866, "cached_tokens": 792}}, "human_critique": null, "stages": [{"id": "extraction", "state": "complete", "reader": "MiniMax-M3.1-Flash", "provider": "minimax"}], "gaps": ["review.independent_reader", "review.machine_critique", "review.human_critique"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "pmid:32995120", "type": "paper", "label": "pmid:32995120"}, {"id": "source:pmid:32995120", "type": "manuscript", "label": "PMID 32995120 full text"}, {"id": "data:geo:GSE12945", "type": "dataset", "label": "GEO GSE12945"}], "activities": [{"id": "activity-reading-sci-2026-11-01", "type": "classification", "used": [{"id": "pmid:32995120", "type": "paper", "label": "pmid:32995120"}, {"id": "source:pmid:32995120", "type": "manuscript", "label": "PMID 32995120 full text"}, {"id": "data:geo:GSE12945", "type": "dataset", "label": "GEO GSE12945"}], "agent": "agent:corpus-assay-engine"}, {"id": "activity-read-32995120", "type": "extraction", "used": ["source:pmid:32995120"], "agent": "agent:minimax-reader"}, {"id": "activity-resolve-GSE12945", "type": "accession_resolution", "used": ["data:geo:GSE12945"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}, {"id": "agent:minimax-reader", "type": "software", "label": "MiniMax literature reader"}], "source_run_id": "reading-sci-2026-11-01", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "pmid:32995120", "parent_hash": "sha256:51078d79780e014081ecc24f3c58dcb5fd7bf6e2ecdd6e07397aa28a45682563", "fork_of": null, "generation": 6}, "gaps": ["asset:data:geo:GSE12945 licence not read", "asset:data:geo:GSE12945 not downloaded", "asset:data:geo:GSE147334 licence not read", "asset:data:geo:GSE147334 not downloaded", "asset:data:geo:GSE17536 licence not read", "asset:data:geo:GSE17536 not downloaded", "asset:data:geo:GSE17537 licence not read", "asset:data:geo:GSE17537 not downloaded", "asset:data:geo:GSE8671 licence not read", "asset:data:geo:GSE8671 not downloaded", "execution.not_run", "method.reader_differs_from_extractor", "review.human_critique", "review.independent_reader", "review.machine_critique"], "assays": [{"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["sed 10 (TEX10) is upregulated in CRC and its knockdown decreases cell proliferation. The TEX10 high", "enriched in the genes downregulated by TEX10 knockdown. Mechanistically, TEX10 interacts with RELA"], "order_basis": "paper_text_order:517", "id": "assay:1"}, {"role": "data_generation", "definition_id": "animal_model", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["creases the growth of CRC cells in vitro and in vivo. Gene set enrichment analysis indicates that", "ight2 × length. After 20 d of injection, the mice were sacrificed and the tumors removed and weighed. Statisti", "19000P). 4‐ to 5‐week‐old female BABL/c nude mice were purchased from Beijing Vital River Laborator", "ight2 × length. After 20 d of injection, the mice were sacrificed and the tumors removed and weighe"], "order_basis": "paper_text_order:775", "id": "assay:2"}, {"role": "analysis", "definition_id": "survival_analysis", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["cox_ph"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["The Cox proportional hazards model was used for analyzing associations of variables with survival."], "order_basis": "consumes_earlier_output", "id": "assay:3"}]}