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pmid:33061846
Hsa_circ_0026416 promotes proliferation and migration in colorectal cancer via miR-346/NFIB axis.
Yahang Liang · Jingbo Shi · Qingsi He · Guorui Sun · Lei Gao · Jianhong Ye · Xiaolong Tang · Hui Qu
Cancer cell international · 2020 doi:10.1186/s12935-020-01593-1
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Complete file
{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:33061846", "kind": "science-record", "revision": 6, "content_hash": "sha256:0ad0cc226dec129483d0ed56b398fc739ab1606a94a8848dab4ebeddb9e5b60d", "created_at": "2026-11-01T15:41:13Z", "updated_at": "2026-11-01T21:23:57Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Hsa_circ_0026416 promotes proliferation and migration in colorectal cancer via miR-346/NFIB axis.", "doi": "10.1186/s12935-020-01593-1", "pmid": "33061846", "authors": ["Yahang Liang", "Jingbo Shi", "Qingsi He", "Guorui Sun", "Lei Gao", "Jianhong Ye", "Xiaolong Tang", "Hui Qu"], "journal": "Cancer cell international", "year": 2020, "abstract": "BACKGROUND: Colorectal cancer (CRC) is one of the most common cancers worldwide. Circular RNAs (circRNAs), a novel class of non-coding RNAs, have been confirmed to be key regulators of many diseases. With many scholars devoted to studying the biological function and mechanism of circRNAs, their mysterious veil is gradually being revealed. In our research, we explored a new circRNA, hsa_circ_0026416, which was identified as upregulated in CRC with the largest fold change (logFC = 3.70) of the evaluated circRNAs via analysing expression profiling data by high throughput sequencing of members of the GEO dataset (GSE77661) to explore the molecular mechanisms of CRC.\nMETHODS: qRT-PCR and western blot analysis were utilized to assess the expression of hsa_circ_0026416, miR-346 and Nuclear Factor I/B (NFIB). CCK-8 and transwell assays were utilized to examine cell proliferation, migration and invasion in vitro, respectively. A luciferase reporter assay was used to verify the combination of hsa_circ_0026416, miR-346 and NFIB. A nude mouse xenograft model was also utilized to determine the role of hsa_circ_0026416 in CRC cell growth in vivo.\nRESULTS: Hsa_circ_0026416 was markedly upregulated in CRC patient tissues and plasma and was a poor prognosis in CRC patients. In addition, the area under the curve (AUC) of hsa_circ_0026416 (0.767) was greater than the AUC of CEA (0.670), CA19-9 (0.592) and CA72-4 (0.575). Functionally, hsa_circ_0026416 promotes cell proliferation, migration and invasion both in vitro and in vivo. Mechanistically, hsa_circ_0026416 may function as a ceRNA via competitively absorbing miR-346 to upregulate the expression of NFIB.\nCONCLUSIONS: In summary, our findings demonstrate that hsa_circ_0026416 is an oncogene in CRC. Hsa_circ_0026416 promotes the progression of CRC via the miR-346/NFIB axis and may represent a potential biomarker for diagnosis and therapy in CRC.", "claims": [{"id": "claim-method", "statement": "The efficiency of hsa_circ_0026416 in the diagnosis of CRC was evaluated by receiver operating characteristic (ROC) curve.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33061846", "locator": "sections[15] \"Statistical analysis\" paragraph 2", "quote": "The efficiency of hsa_circ_0026416 in the diagnosis of CRC was evaluated by receiver operating characteristic (ROC) curve."}], "state": "complete"}, {"id": "claim-data-access", "statement": "All data generated or analysed during this study are included in the published article and are available in Gene Expression Omnibus (GEO), circBase, The Human Protein Atlas, RegRNA2.0, CircInteractome, miRDB, miRWalk, starBase v3.0 and TargetScan 7.1. 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No dataset was downloaded and no licence was read."}}, "can_run": {"dsl_operations": ["logrank_test"], "assets": ["source:pmid:33061846", "data:geo:GSE77661", "asset:table:pmid:33061846:1", "asset:table:pmid:33061846:2"], "steps": ["run_analysis"]}, "must_build": {"dsl_operations": [], "converters": [], "readers": [], "capabilities": [], "data_gaps": ["data:geo:GSE77661"]}, "gaps": []}, "review": {"state": "partial", "machine_critique": {"kind": "extraction_review", "reader": {"model": "MiniMax-M3.1-Flash", "provider": "minimax", "provider_family": "minimax", "read_at": "2026-10-31T18:23:20Z", "independence": "single_reader"}, "evidence_check": {"method": "verbatim", "availability": "verbatim"}, "calls": 3, "usage": {"prompt_tokens": 43980, "completion_tokens": 1522, "cached_tokens": 784}}, "human_critique": null, "stages": [{"id": "extraction", "state": "complete", "reader": "MiniMax-M3.1-Flash", "provider": "minimax"}], "gaps": ["review.independent_reader", "review.machine_critique", "review.human_critique"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "pmid:33061846", "type": "paper", "label": "pmid:33061846"}, {"id": "source:pmid:33061846", "type": "manuscript", "label": "PMID 33061846 full text"}, {"id": "data:geo:GSE77661", "type": "dataset", "label": "GEO GSE77661"}], "activities": [{"id": "activity-reading-sci-2026-11-01", "type": "classification", "used": [{"id": "pmid:33061846", "type": "paper", "label": "pmid:33061846"}, {"id": "source:pmid:33061846", "type": "manuscript", "label": "PMID 33061846 full text"}, {"id": "data:geo:GSE77661", "type": "dataset", "label": "GEO GSE77661"}], "agent": "agent:corpus-assay-engine"}, {"id": "activity-read-33061846", "type": "extraction", "used": ["source:pmid:33061846"], "agent": "agent:minimax-reader"}, {"id": "activity-resolve-GSE77661", "type": "accession_resolution", "used": ["data:geo:GSE77661"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}, {"id": "agent:minimax-reader", "type": "software", "label": "MiniMax literature reader"}], "source_run_id": "reading-sci-2026-11-01", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "pmid:33061846", "parent_hash": "sha256:8933887e50b7fa6a16bea022e3ca4a84d055729a5806573a8cfed1e90728ad7d", "fork_of": null, "generation": 5}, "gaps": ["asset:data:geo:GSE77661 licence not read", "asset:data:geo:GSE77661 not downloaded", "execution.not_run", "method.reader_named_unmapped", "review.human_critique", "review.independent_reader", "review.machine_critique"], "assays": [{"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["ular mechanisms of CRC. METHODS: qRT-PCR and western blot analysis were utilized to assess the express"], "order_basis": "paper_text_order:745", "id": "assay:1"}, {"role": "data_generation", "definition_id": "apoptosis_assay", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["6416, miR-346 and Nuclear Factor I/B (NFIB). CCK-8 and transwell assays were utilized to examin"], "order_basis": "paper_text_order:866", "id": "assay:2"}, {"role": "data_generation", "definition_id": "animal_model", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["role of hsa_circ_0026416 in CRC cell growth in vivo. RESULTS: Hsa_circ_0026416 was markedly upre", "on, migration and invasion both in vitro and in vivo. Mechanistically, hsa_circ_0026416 may funct", "f hsa_circ_0026416, miR-346 and NFIB. A nude mouse xenograft model was also utilized to determi"], "order_basis": "paper_text_order:1094", "id": "assay:3"}, {"role": "analysis", "definition_id": "survival_analysis", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["logrank_test"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 0.8, "evidence": ["log-rank"], "order_basis": "consumes_earlier_output", "id": "assay:4"}]}