Heat Stress Triggers Differential Protein Accumulation in the Extracellular Matrix of Sorghum Cell Suspension Cultures.

{"sci_version": "1.0", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:33105781", "kind": "science-record", "revision": 4, "content_hash": "sha256:ac61be037141a4a1819cd2cc2f7c15ad5c61d03c38873d57321422327f4b4a06", "created_at": "2026-11-01T15:41:14Z", "updated_at": "2026-11-01T21:24:00Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Heat Stress Triggers Differential Protein Accumulation in the Extracellular Matrix of Sorghum Cell Suspension Cultures.", "doi": "10.3390/proteomes8040029", "pmid": "33105781", "authors": ["Mamosa G Ngcala", "Tatenda Goche", "Adrian P Brown", "Stephen Chivasa", "Rudo Ngara"], "journal": "Proteomes", "year": 2020, "abstract": "Plants reprogram gene expression as an adaptive response to survive high temperatures. While the identity and functions of intracellular heat stress-responsive proteins have been extensively studied, the heat response of proteins secreted to the extracellular matrix is unknown. Here, we used Sorghum bicolor, a species adapted for growth in hot climates, to investigate the extracellular heat-induced responses. When exposed to 40 C for 72 h, heat-sensitive Arabidopsis cell suspension cultures died, while ICSB338 sorghum cell cultures survived by activation of a transcriptional response characterized by the induction of HSP70 and HSP90 genes. Quantitative proteomic analysis of proteins recovered from cell culture medium revealed specific heat stress-induced protein accumulation within the sorghum secretome. Of the 265 secreted proteins identified, 31 responded to heat (2-fold change), with 84% possessing a predicted signal peptide for targeting to the classical secretory pathway. The differentially accumulated proteins have putative functions in metabolism, detoxification, and protein modifications. A germin (SORBI_3003G427700) was highly heat-inducible at both protein and gene level. Overall, our study reveals new insights into sorghum responses to heat and provides a useful resource of extracellular proteins that could serve as targets for developing thermotolerant crops. Data are available via ProteomeXchange with identifier PXD021536.", "claims": [{"id": "claim-method", "statement": "A Student’s t-test at p ≤ 0.05 was used to calculate the probability values of the differentially accumulated proteins and a cutoff threshold of two-fold was applied to filter the dataset.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33105781", "locator": "sections[6] \"2.4. 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This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:PXD021536"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:PXD021536"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:33105781", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.3390/proteomes8040029", "sha256": "sha256:fd0652f4677686a49602ac2689878f6697a28bc8344a1cd4475b5df56ade3564", "bytes": 61364, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.3390/proteomes8040029", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC7709130", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:PXD021536", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=PXD021536", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "PXD021536", "version": null, "note": "Data are available via ProteomeXchange with identifier PXD021536.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "Data are available via ProteomeXchange with identifier PXD021536.", "access_statement_kind": "data_availability_statement", "sample_accessions": [], "sample_count": null, "group_sizes": null, "group_sizes_basis": null, "platform": null, "taxon": null, "title": "Heat stress triggers protein secretion into the extracellular matrix of sorghum cell suspension cultures", "resolution_checked_at": "2026-10-31T18:35:55Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "asset:table:pmid:33105781:1", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:a58d250e95262c059a2c9e432bf17b91194eaa0eda7afce87be9098fb5f24bae", "bytes": 5241, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[12]/table-wrap[1]#proteomes-08-00029-t001", "version": null, "note": "List of differentially accumulated sorghum secreted proteins in response to heat stress.", "structure": {"pmcid": "PMC7709130", "section_index": 12, "section_title": "3.3. Heat Stress Triggers Differential Protein Accumulation in the Sorghum Extracellular Matrix (ECM)", "row_count": 36, "column_count": 8}, "table": {"label": "Table 1", "caption": "List of differentially accumulated sorghum secreted proteins in response to heat stress.", "rows": [["Prot No. a", "Accession b", "Protein Name", "Ratio c", "SD d", "p-Value e", "Signal Peptide f", "Family Name g"], ["Metabolism"], ["11", "A0A1B6QCB0 *", "Alpha-amylase OS = Sorghum bicolorGN = SORBI_3002G190500", "2.84", "0.22", "4.89 × 10−5", "+", "Alpha-amylase, plant"], ["36", "C5XB39 *", "Uncharacterized protein OS = Sorghum bicolorGN = SORBI_3002G055700", "2.41", "0.20", "3.80 × 10−4", "+", "Glycosyl hydrolase superfamily"], ["92", "C5XD22", "Uncharacterized protein OS = Sorghum bicolor GN = SORBI_3002G356800", "−2.32", "0.05", "2.54 × 10−3", "+", "Lipase_GDSL domain"], ["106", "A0A1W0VY92 *", "Uncharacterized protein OS = Sorghum bicolor GN = SORBI_3003G205900", "−2.31", "0.08", "4.37 × 10−4", "+", "Lipase_GDSL domain"], ["113", "A0A1B6Q537", "Uncharacterized protein OS = Sorghum bicolor GN = SORBI_3003G244600", "2.02", "0.47", "5.86 × 10−3", "-", "Glycosyl hydrolase, family 18"], ["125", "C5WSY5", "Uncharacterized protein OS = Sorghum bicolor GN = SORBI_3001G014700", "−2.51", "0.07", "4.89 × 10−3", "+", "Glycoside hydrolase, family 17"], ["127", "C5Z8T4 *", "Xyloglucan endotransglucosylase/hydrolase OS = Sorghum bicolor GN = SORBI_3010G246600", "2.47", "0.30", "4.69 × 10−4", "+", "Xyloglucan endotransglucosylase/hydrolase"], ["250", "C5 × 578", "Uncharacterized protein OS = Sorghum bicolor GN = SORBI_3002G128000", "2.31", "0.33", "1.32 × 10−2", "+", "SGNH hydrolase superfamily"], ["279", "A0A1B6QEM0", "Xyloglucan endotransglucosylase/hydrolase OS = Sorghum bicolor GN = SORBI_3002G324100", "−2.50", "0.10", "2.61 ×10 −2", "+", "Xyloglucan endotransglucosylase/hydrolase"], ["Detoxification & Defence"], ["15", "C5X5K6", "Peroxidase OS = Sorghum bicolor GN = SORBI_3002G416700", "−2.17", "0.02", "1.34 × 10−2", "+", "Plant peroxidase"], ["50", "C5X040 *", "Peroxidase OS = Sorghum bicolor GN = SORBI_3001G080300", "2.06", "0.27", "5.18 × 10−4", "+", "Plant peroxidase"], ["58", "C5YVR0 *", "Superoxide dismutase OS = Sorghum bicolor GN = SORBI_3009G093200", "2.35", "0.38", "8.83 × 10−4", "-", "Manganese/iron superoxide dismutase"], ["60", "C5XHF1 *", "Uncharacterized protein OS = Sorghum bicolor GN = SORBI_3003G136200", "2.08", "0.24", "2.38 × 10−4", "+", "Germin"], ["108", "C5XL59 *", "Peroxidase OS = Sorghum bicolor GN = SORBI_3003G024700", "−3.23", "0.07", "2.70 × 10−5", "-", "Plant peroxidase"], ["137", "C5XHX2 *", "Uncharacterized protein OS = Sorghum bicolor GN = SORBI_3003G427700", "2.85", "0.43", "4.85 × 10−4", "+", "Germin"], ["154", "C5XJT8 *", "Uncharacterized protein OS = Sorghum bicolor GN = SORBI_3003G156400", "2.17", "0.29", "4.45 × 10−4", "+", "Thioredoxin-like superfamily"], ["166", "C5YD83", "Uncharacterized protein OS = Sorghum bicolor GN = SORBI_3006G031200", "2.09", "0.45", "7.25 × 10−3", "+", "Thioredoxin-like superfamily"], ["Protein Degradation"], ["47", "C5XHP9 *", "Uncharacterized protein OS = Sorghum bicolor GN = SORBI_3003G419500", "2.18", "0.13", "5.94× 10−5", "+", "Aspartic peptidase A1 family"], ["49", "C5XHP7 *", "Uncharacterized protein OS = Sorghum bicolor GN = SORBI_3003G419300", "2.74", "0.20", "3.77 × 10−5", "-", "Aspartic peptidase A1 family"], ["74", "C5WVG9 *", "Cysteine proteinase inhibitor OS = Sorghum bicolor GN = SORBI_3001G324800", "2.19", "0.17", "8.33 × 10−5", "+", "Cysteine proteinase inhibitor"], ["153", "C5Z1X3", "Uncharacterized protein OS = Sorghum bicolor GN = SORBI_3010G268400", "−2.10", "0.03", "5.84 × 10−3", "+", "Aspartic peptidase A1 family"], ["215", "C5XAQ7", "Uncharacterized protein OS = Sorghum bicolor GN = SORBI_3002G326100", "−2.05", "0.08", "4.50 × 10−2", "+", "Aspartic peptidase A1 family"], ["219", "A0A1B6Q242", "Uncharacterized protein OS = Sorghum bicolor GN = SORBI_3003G085300", "−2.00", "0.03", "5.30 × 10−3", "+", "Bowman-Birk type proteinase inhibitor"], ["225", "C5XGM0", "Cysteine proteinase inhibitor OS = Sorghum bicolor GN = SORBI_3003G126800", "2.16", "0.42", "9.39 × 10−3", "+", "Cysteine proteinase inhibitor"], ["259", "A0A1B6Q6G6", "Uncharacterized protein OS = Sorghum bicolor GN = SORBI_3003G314800", "−2.00", "0.11", "2.50 × 10−2", "+", "Aspartic peptidase A1 family"], ["Unclassified"], ["26", "A0A1Z5R915 *", "Purple acid phosphatase OS = Sorghum bicolor GN = SORBI_3007G091100", "−4.18", "0.04", "2.42 × 10−4", "-", "Purple acid phosphatase-like"], ["33", "C5XBP7", "Uncharacterized protein OS = Sorghum bicolor GN = SORBI_3002G343600", "−2.55", "0.06", "3.77 × 10−3", "+", "Leucine-rich repeat domain superfamily"], ["34", "A0A1B6PLT5 *", "Uncharacterized protein OS = Sorghum bicolor GN = SORBI_3006G133000", "−2.83", "0.05", "5.29 × 10−4", "+", "Domain of unknown function DUF642"], ["101", "C5Y2R8 *", "Uncharacterized protein OS = 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