DUS4L Silencing Suppresses Cell Proliferation and Promotes Apoptosis in Human Lung Adenocarcinoma Cell Line A549.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:33116848", "kind": "science-record", "revision": 7, "content_hash": "sha256:0ebadd6fb1fc88ec0bc274770495b098778d3378b0bc2a11699d91409807ee3d", "created_at": "2026-11-01T15:41:14Z", "updated_at": "2026-11-01T22:02:25Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "DUS4L Silencing Suppresses Cell Proliferation and Promotes Apoptosis in Human Lung Adenocarcinoma Cell Line A549.", "doi": "10.2147/CMAR.S265671", "pmid": "33116848", "authors": ["Zheng Li", "Ci Yin", "Bin Li", "Qi-Yao Yu", "Wen-Jie Mao", "Jie Li", "Jun-Ping Lin", "Yu-Qi Meng", "Hai-Ming Feng", "Tao Jing"], "journal": "Cancer management and research", "year": 2020, "abstract": "PURPOSE: This study aims to investigate the potential role of DUS4L (dihydrouridine synthase 4 like) in lung adenocarcinoma (LUAD) and explore its associated pathways in human LUAD.\nMETHODS: Firstly, we evaluated the relationships between clinicopathological characteristics and DUS4L expression via analysis of TCGA RNA sequencing data and other publicly available databases. Then, DUS4L was effectively silenced in LUAD cell line A549 using the lentiviral shRNA (short-hairpin RNA) transfection to assess its effects on cell proliferation, cycle and apoptosis in LUAD cells. RNA-seq technology was applied to shDUS4L and shCtrl-transfected cells to generate the corresponding gene expression profiles. Differentially expressed genes (DEGs) were identified using the DESeq2 program package. Also, DEGs were subjected to Gene Ontology (GO) and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis to explore the associated molecular signaling pathways and relevant biological functions.\nRESULTS: Analysis of TCGA data revealed that DUS4L was highly upregulated in LUAD tissues which was related to clinical T and TNM stages of LUAD. The knockdown of DUS4L effectively inhibited cell proliferation and promoted apoptosis in A549 cells. Furthermore, the DEGs between the shDUS4L and shCtrl A549 cells were mainly enriched in biological processes associated with spliceosome, ribosome, RNA catabolic process, ncRNA (non-coding RNA) processing, and p53 signaling pathway.\nCONCLUSION: Altogether, our results suggest that DUS4L is significantly associated with tumorigenesis and could be utilized as a novel biomarker and therapeutic target for LUAD.", "claims": [{"id": "claim-method", "statement": "One-way ANOVA was applied for comparisons between multiple groups and the difference between the two groups was subjected to unpaired students’ t-tests.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33116848", "locator": "sections[13] \"Statistical Analysis\" paragraph 2", "quote": "One-way ANOVA was applied for comparisons between multiple groups and the difference between the two groups was subjected to unpaired students’ t-tests."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The RNA-seq mRNA expression profiles (Workflow Type: HTSeq-FPKM) of DUS4L in LUAD, including a total of 535 LUAD and 59 adjacent normal tissues, and the corresponding clinical data information were extracted from TCGA Genomic Data Commons (GDC) Portal (https://gdc.cancer.gov/).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33116848", "locator": "sections[3] \"Bioinformatic Analysis\" paragraph 2", "quote": "The RNA-seq mRNA expression profiles (Workflow Type: HTSeq-FPKM) of DUS4L in LUAD, including a total of 535 LUAD and 59 adjacent normal tissues, and the corresponding clinical data information were extracted from TCGA Genomic Data Commons (GDC) Portal (https://gdc.cancer.gov/)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:33116848"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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