miR-766-3p Targeting BCL9L Suppressed Tumorigenesis, Epithelial-Mesenchymal Transition, and Metastasis Through the β-Catenin Signaling Pathway in Osteosarcoma Cells.

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The purpose of the present study was to elucidate the relationship between miR-766-3p and development of osteosarcoma and explore the potential mechanism. In this study, we found that miR-766-3p was the most downregulated miRNA by analyzing GSE65071 from the GEO database. miR-766-3p was lowly expressed in OS tissue samples and cells, and high miR-766-3p expression repressed the malignant level of OS, including cell proliferation, EMT, migration, and invasion in vitro and in vivo. B-Cell Lymphoma 9-Like Protein (BCL9L) was negatively associated with miR-766-3p expression in OS cells and tissue samples and was validated as the downstream target by luciferase reporter assay and western blotting. Rescue experiment indicated that BCL9L could restore the influence of miR-766-3p on OS cells. In addition, the β-Catenin/TCF-4 signal pathway was demonstrated to be related to the miR-766-3p/BCL9L axis. In summary, miR-766-3p, a negative regulator of BCL9L, plays the role of tumor metastasis suppressor via the β-catenin signaling pathway in the progression of OS.", "claims": [{"id": "claim-method", "statement": "In order to detect miRNAs expression in OS clinical tissues, the data from the GEO database (GSE65071) were analyzed using the R package limma (Wendy Allen-Rhoades et al., 2015).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33117820", "locator": "sections[18] \"miR-766-3p Expression Was Down-Regulated in OS Cells and Tissue Samples\" paragraph 2", "quote": "In order to detect miRNAs expression in OS clinical tissues, the data from the GEO database (GSE65071) were analyzed using the R package limma (Wendy Allen-Rhoades et al., 2015)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "All datasets generated for this study are included in the article.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33117820", "locator": "sections[27] \"Data Availability Statement\" paragraph 2", "quote": "All datasets generated for this study are included in the article."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:33117820"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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B-Cell Lymphoma 9-Like Protein (BCL9L) was"], "order_basis": "paper_text_order:743", "id": "assay:1"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["ream target by luciferase reporter assay and western blotting. Rescue experiment indicated that BCL9L coul"], "order_basis": "paper_text_order:951", "id": "assay:2"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["on) (Manassas, United States). OS cells were cultured in DMEM/F12 medium (Life Technologies, NY) with", "80–85% confluence. The hFOB 1.19 cells were cultured in DMEM/F-12 (Life Technologies, NY) containing", "cells, including Saos-2, MG63, HOS, 143B and U2OS, and hFOB1.19 were purchased from the ATCC ("], "order_basis": "paper_text_order:2076", "id": "assay:3"}, {"role": "data_generation", "definition_id": "qpcr", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["s according to the data from GSE65071. 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