Identification prognosis-associated immune genes in colon adenocarcinoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:33140821", "kind": "science-record", "revision": 7, "content_hash": "sha256:4b6bde2ebacc0fefdc91379e058f71df8b5091d04559f777dcd3ba95513ae589", "created_at": "2026-11-01T15:41:14Z", "updated_at": "2026-11-01T22:02:26Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Identification prognosis-associated immune genes in colon adenocarcinoma.", "doi": "10.1042/BSR20201734", "pmid": "33140821", "authors": ["Yandong Miao", "Jiangtao Wang", "Xueping Ma", "Yuan Yang", "Denghai Mi"], "journal": "Bioscience reports", "year": 2020, "abstract": "Colon adenocarcinoma (COAD) is one of the most prevalent malignant tumors worldwide. Immune genes (IGs) have a considerable correlation with tumor initiation and prognosis. The present paper aims to identify the prognosis value of IGs in COAD and conduct a prognosis model for clinical utility. Gene expression data of COAD were downloaded from The Cancer Genome Atlas (TCGA), screening and analyzing differentially expressed IGs by bioinformatics. Core genes were screened by univariate and multivariate Cox regression analyses. Survival analysis was appraised by the Kaplan-Meier method and the log-rank test. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, and Gene Set Enrichment Analysis (GSEA) were used to identify IGs' relevant signal pathways. We predicted the overall survival (OS) by nomogram. Finally, a prognosis model was conducted based on 12 IGs (SLC10A2, CXCL3, NOX4, FABP4, ADIPOQ, IGKV1-33, IGLV6-57, INHBA, UCN, VIP, NGFR, and TRDC). The risk score was an independent prognostic factor, and a nomogram could accurately predict the OS of individual COAD patients. These results were validated in GSE39582, GSE12945, and GSE103479 cohorts. Functional enrichment analysis demonstrated that these IGs are mainly enriched in hormone secretion, hormone transport, lipid transport, cytokine-cytokine receptor interaction, and peroxisome proliferators-activated receptor signaling pathway. In summary, the risk score is an independent prognostic biomarker. We also excavated several IGs related to COAD's survival and maybe potential biomarkers for COAD diagnosis and treatment.", "claims": [{"id": "claim-method", "statement": "Screening differentially expressed genes, IGs, transcription factors (TFs) was undertaken by the Wilcoxon Test [16], using R-package ‘limma’ ‘edgeR.’False-discovery rate (FDR) < 0.05 and Log2 | (fold change, FC) | > 1.5 were set as the cutoffs.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33140821", "locator": "sections[5] \"Identification of differential expression of genes, IGs, and transcription factors\" paragraph 2", "quote": "Screening differentially expressed genes, IGs, transcription factors (TFs) was undertaken by the Wilcoxon Test [16], using R-package ‘limma’ ‘edgeR.’False-discovery rate (FDR) < 0.05 and Log2 | (fold change, FC) | > 1.5 were set as the cutoffs."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The authors certify that all the original data in this research could be obtained from public database. Both the transcriptome and clinical data of COAD were obtained from the TCGA database (https://tcga-data.nci.nih.gov/tcga/). The data of training group are available at NCBI GEO (GSE39582 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39582, GSE12945 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE12945, and GSE103479 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE103479). Other data that were used to support the findings of the present study are included within the supplementary information files. All the raw data in the present study are available from the first author or corresponding author upon request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33140821", "locator": "sections[18] \"Data Availability\" paragraph 2", "quote": "The authors certify that all the original data in this research could be obtained from public database. Both the transcriptome and clinical data of COAD were obtained from the TCGA database (https://tcga-data.nci.nih.gov/tcga/). The data of training group are available at NCBI GEO (GSE39582 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39582, GSE12945 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE12945, and GSE103479 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE103479). Other data that were used to support the findings of the present study are included within the supplementary information files. All the raw data in the present study are available from the first author or corresponding author upon request."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:33140821"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE103479"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "blocked", "blocked_on": "method:group_comparison_wilcoxon", "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE103479"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": ["step:run_analysis blocked on method:group_comparison_wilcoxon"]}, "data": {"state": "partial", "assets": [{"id": "source:pmid:33140821", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1042/BSR20201734", "sha256": "sha256:3be7f34178663d4411d6768b94b922a7f729033067cb41c0c0d10b97772d8119", "bytes": 27597, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1042/BSR20201734", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC7670579", "source": "europe_pmc_fulltext", "coverage": "whole_body_without_references", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE103479", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE103479", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE103479", "version": null, "note": "The authors certify that all the original data in this research could be obtained from public database. Both the transcriptome and clinical data of COAD were obtained from the TCGA database (https://tcga-data.nci.nih.gov/tcga/). The data of training group are available at NCBI GEO (GSE39582 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39582, GSE12945 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE12945, and GSE103479 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE103479). Other data that were used to support the findings of the present study are included within the supplementary information files. All the raw data in the present study are available from the first author or corresponding author upon request.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The authors certify that all the original data in this research could be obtained from public database. Both the transcriptome and clinical data of COAD were obtained from the TCGA database (https://tcga-data.nci.nih.gov/tcga/). The data of training group are available at NCBI GEO (GSE39582 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39582, GSE12945 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE12945, and GSE103479 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE103479). Other data that were used to support the findings of the present study are included within the supplementary information files. All the raw data in the present study are available from the first author or corresponding author upon request.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM2772122", "GSM2772123", "GSM2772124", "GSM2772125", "GSM2772126", "GSM2772127", "GSM2772128", "GSM2772129", "GSM2772130", "GSM2772131", "GSM2772132", "GSM2772133", "GSM2772134", "GSM2772135", "GSM2772136", "GSM2772137", "GSM2772138", "GSM2772139", "GSM2772140", "GSM2772141", "GSM2772142", "GSM2772143", "GSM2772144", "GSM2772145", "GSM2772146", "GSM2772147", "GSM2772148", "GSM2772149", "GSM2772150", "GSM2772151", "GSM2772152", "GSM2772153", "GSM2772154", "GSM2772155", "GSM2772156", "GSM2772157", "GSM2772158", "GSM2772159", "GSM2772160", "GSM2772161", "GSM2772162", "GSM2772163", "GSM2772164", "GSM2772165", "GSM2772166", "GSM2772167", "GSM2772168", "GSM2772169", "GSM2772170", "GSM2772171", "GSM2772172", "GSM2772173", "GSM2772174", "GSM2772175", "GSM2772176", "GSM2772177", "GSM2772178", "GSM2772179", "GSM2772180", "GSM2772181", "GSM2772182", "GSM2772183", "GSM2772184", "GSM2772185", "GSM2772186", "GSM2772187", "GSM2772188", "GSM2772189", "GSM2772190", "GSM2772191", "GSM2772192", "GSM2772193", "GSM2772194", "GSM2772195", "GSM2772196", "GSM2772197", "GSM2772198", "GSM2772199", "GSM2772200", "GSM2772201", "GSM2772202", "GSM2772203", "GSM2772204", "GSM2772205", "GSM2772206", "GSM2772207", "GSM2772208", "GSM2772209", "GSM2772210", "GSM2772211", "GSM2772212", "GSM2772213", "GSM2772214", "GSM2772215", "GSM2772216", "GSM2772217", "GSM2772218", "GSM2772219", "GSM2772220", "GSM2772221", "GSM2772222", "GSM2772223", "GSM2772224", "GSM2772225", "GSM2772226", "GSM2772227", "GSM2772228", "GSM2772229", "GSM2772230", "GSM2772231", "GSM2772232", "GSM2772233", "GSM2772234", "GSM2772235", "GSM2772236", "GSM2772237", "GSM2772238", "GSM2772239", "GSM2772240", "GSM2772241", "GSM2772242", "GSM2772243", "GSM2772244", "GSM2772245", "GSM2772246", "GSM2772247", "GSM2772248", "GSM2772249", "GSM2772250", "GSM2772251", "GSM2772252", "GSM2772253", "GSM2772254", "GSM2772255", "GSM2772256", "GSM2772257", "GSM2772258", "GSM2772259", "GSM2772260", "GSM2772261", "GSM2772262", "GSM2772263", "GSM2772264", "GSM2772265", "GSM2772266", "GSM2772267", "GSM2772268", "GSM2772269", "GSM2772270", "GSM2772271", "GSM2772272", "GSM2772273", "GSM2772274", "GSM2772275", "GSM2772276", "GSM2772277"], "sample_count": 156, "group_sizes": null, "group_sizes_basis": null, "platform": "23985", "taxon": "Homo sapiens", "title": "Gene expression data from stage II and III treated and untreated colorectal cancer patients", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE12945", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE12945", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE12945", "version": null, "note": "The authors certify that all the original data in this research could be obtained from public database. Both the transcriptome and clinical data of COAD were obtained from the TCGA database (https://tcga-data.nci.nih.gov/tcga/). The data of training group are available at NCBI GEO (GSE39582 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39582, GSE12945 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE12945, and GSE103479 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE103479). Other data that were used to support the findings of the present study are included within the supplementary information files. All the raw data in the present study are available from the first author or corresponding author upon request.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The authors certify that all the original data in this research could be obtained from public database. Both the transcriptome and clinical data of COAD were obtained from the TCGA database (https://tcga-data.nci.nih.gov/tcga/). The data of training group are available at NCBI GEO (GSE39582 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39582, GSE12945 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE12945, and GSE103479 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE103479). Other data that were used to support the findings of the present study are included within the supplementary information files. All the raw data in the present study are available from the first author or corresponding author upon request.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM324715", "GSM324716", "GSM324717", "GSM324718", "GSM324719", "GSM324720", "GSM324721", "GSM324722", "GSM324723", "GSM324724", "GSM324725", "GSM324726", "GSM324727", "GSM324728", "GSM324729", "GSM324730", "GSM324731", "GSM324732", "GSM324733", "GSM324734", "GSM324735", "GSM324736", "GSM324737", "GSM324738", "GSM324739", "GSM324740", "GSM324741", "GSM324742", "GSM324743", "GSM324744", "GSM324745", "GSM324746", "GSM324747", "GSM324748", "GSM324749", "GSM324750", "GSM324751", "GSM324752", "GSM324753", "GSM324754", "GSM324755", "GSM324756", "GSM324757", "GSM324758", "GSM324759", "GSM324760", "GSM324761", "GSM324762", "GSM324763", "GSM324764", "GSM324765", "GSM324766", "GSM324767", "GSM324768", "GSM324769", "GSM324770", "GSM324771", "GSM324772", "GSM324773", "GSM324774", "GSM324775", "GSM324776"], "sample_count": 62, "group_sizes": null, "group_sizes_basis": null, "platform": "96", "taxon": "Homo sapiens", "title": "Expression data from colorectal cancers", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE39582", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39582", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE39582", "version": null, "note": "The authors certify that all the original data in this research could be obtained from public database. Both the transcriptome and clinical data of COAD were obtained from the TCGA database (https://tcga-data.nci.nih.gov/tcga/). The data of training group are available at NCBI GEO (GSE39582 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39582, GSE12945 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE12945, and GSE103479 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE103479). Other data that were used to support the findings of the present study are included within the supplementary information files. All the raw data in the present study are available from the first author or corresponding author upon request.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The authors certify that all the original data in this research could be obtained from public database. Both the transcriptome and clinical data of COAD were obtained from the TCGA database (https://tcga-data.nci.nih.gov/tcga/). The data of training group are available at NCBI GEO (GSE39582 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39582, GSE12945 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE12945, and GSE103479 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE103479). Other data that were used to support the findings of the present study are included within the supplementary information files. All the raw data in the present study are available from the first author or corresponding author upon request.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM1681353", "GSM1681354", "GSM1681355", "GSM1681356", "GSM1681357", "GSM1681358", "GSM1681359", "GSM1681360", "GSM1681361", "GSM1681362", "GSM1681363", "GSM1681364", "GSM1681365", "GSM1681366", "GSM1681367", "GSM1681368", "GSM1681369", "GSM1681370", "GSM1681371", "GSM971957", "GSM971958", "GSM971959", "GSM971960", "GSM971961", "GSM971962", "GSM971963", "GSM971964", "GSM971965", "GSM971966", "GSM971967", "GSM971968", "GSM971969", "GSM971970", "GSM971971", "GSM971972", "GSM971973", "GSM971974", "GSM971975", "GSM971976", "GSM971977", "GSM971978", "GSM971979", "GSM971980", "GSM971981", "GSM971982", "GSM971983", "GSM971984", "GSM971985", "GSM971986", "GSM971987", "GSM971988", "GSM971989", "GSM971990", "GSM971991", "GSM971992", "GSM971993", "GSM971994", "GSM971995", "GSM971996", "GSM971997", "GSM971998", "GSM971999", "GSM972000", "GSM972001", "GSM972002", "GSM972003", "GSM972004", "GSM972005", "GSM972006", "GSM972007", "GSM972008", "GSM972009", "GSM972010", "GSM972011", "GSM972012", "GSM972013", "GSM972014", "GSM972015", "GSM972016", "GSM972017", "GSM972018", "GSM972019", "GSM972020", "GSM972021", "GSM972022", "GSM972023", "GSM972024", "GSM972025", "GSM972026", "GSM972027", "GSM972028", "GSM972029", "GSM972030", "GSM972031", "GSM972032", "GSM972033", "GSM972034", "GSM972035", "GSM972036", "GSM972037", "GSM972038", "GSM972039", "GSM972040", "GSM972041", "GSM972042", "GSM972043", "GSM972044", "GSM972045", "GSM972046", "GSM972047", "GSM972048", "GSM972049", "GSM972050", "GSM972051", "GSM972052", "GSM972053", "GSM972054", "GSM972055", "GSM972056", "GSM972057", "GSM972058", "GSM972059", "GSM972060", "GSM972061", "GSM972062", "GSM972063", "GSM972064", "GSM972065", "GSM972066", "GSM972067", "GSM972068", "GSM972069", "GSM972070", "GSM972071", "GSM972072", "GSM972073", "GSM972074", 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The physical bench the paper used is not, so a physical validation cannot be run here."}, "data": {"tier": "R1", "reachable": true, "accessions": ["GSE39582", "GSE12945", "GSE103479"], "resolved_accessions": ["GSE39582", "GSE12945", "GSE103479"], "statements": ["The authors certify that all the original data in this research could be obtained from public database. Both the transcriptome and clinical data of COAD were obtained from the TCGA database (https://tcga-data.nci.nih.gov/tcga/). The data of training group are available at NCBI GEO (GSE39582 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39582, GSE12945 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE12945, and GSE103479 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE103479). Other data that were used to support the findings of the present study are included within the supplementary information files. All the raw data in the present study are available from the first author or corresponding author upon request."], "basis": "The paper names GSE39582, GSE12945, GSE103479 in Gene Expression Omnibus (GEO) and the accession resolves in the repository. No dataset was downloaded and no licence was read."}}, "can_run": {"dsl_operations": [], "assets": ["source:pmid:33140821", "data:geo:GSE103479", "asset:table:pmid:33140821:1", "asset:table:pmid:33140821:2", "asset:table:pmid:33140821:3"], "steps": []}, "must_build": {"dsl_operations": [{"operation": "group_comparison_wilcoxon", "unlocks_papers": null, "priority": null}], "converters": [], "readers": [], "capabilities": [], "data_gaps": ["data:geo:GSE103479"]}, "gaps": []}, "review": {"state": "partial", "machine_critique": {"kind": "extraction_review", "reader": {"model": "MiniMax-M3.1-Flash", "provider": "minimax", "provider_family": "minimax", "read_at": "2026-10-31T18:24:05Z", "independence": "single_reader"}, "evidence_check": {"method": "verbatim", "availability": "verbatim"}, "calls": 3, "usage": {"prompt_tokens": 21548, "completion_tokens": 2070, "cached_tokens": 784}}, "human_critique": null, "stages": [{"id": "extraction", "state": "complete", "reader": "MiniMax-M3.1-Flash", "provider": "minimax"}], "gaps": ["review.independent_reader", "review.machine_critique", "review.human_critique"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "pmid:33140821", "type": "paper", "label": "pmid:33140821"}, {"id": "source:pmid:33140821", "type": "manuscript", "label": "PMID 33140821 full text"}, {"id": "data:geo:GSE103479", "type": "dataset", "label": "GEO GSE103479"}], "activities": [{"id": "activity-reading-sci-2026-11-01", "type": "classification", "used": [{"id": "pmid:33140821", "type": "paper", "label": "pmid:33140821"}, {"id": "source:pmid:33140821", "type": "manuscript", "label": "PMID 33140821 full text"}, {"id": "data:geo:GSE103479", "type": "dataset", "label": "GEO GSE103479"}], "agent": "agent:corpus-assay-engine"}, {"id": "activity-read-33140821", "type": "extraction", "used": ["source:pmid:33140821"], "agent": "agent:minimax-reader"}, {"id": "activity-resolve-GSE103479", "type": "accession_resolution", "used": ["data:geo:GSE103479"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}, {"id": "agent:minimax-reader", "type": "software", "label": "MiniMax literature reader"}], "source_run_id": "reading-sci-2026-11-01", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "pmid:33140821", "parent_hash": "sha256:06bc70cf8e7aa3e25993cdcc882884462daf38b3d86bc5b3493076832bdb0535", "fork_of": null, "generation": 6}, "gaps": ["asset:data:geo:GSE103479 licence not read", "asset:data:geo:GSE103479 not downloaded", "asset:data:geo:GSE12945 licence not read", "asset:data:geo:GSE12945 not downloaded", "asset:data:geo:GSE39582 licence not read", "asset:data:geo:GSE39582 not downloaded", "execution.not_run", "method.group_comparison_wilcoxon", "review.human_critique", "review.independent_reader", "review.machine_critique", "step:run_analysis blocked on method:group_comparison_wilcoxon"], "assays": [{"role": "analysis", "definition_id": "group_comparison", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["Screening differentially expressed genes, IGs, transcription factors (TFs) was undertaken by the Wilcoxon Test [16], using R-package ‘limma’ ‘edgeR.’False-discovery rate (FDR) < 0.05 and Log2 | (fold change, FC) | > 1.5 were set as the cutoffs."], "order_basis": "analysis_only", "id": "assay:1"}]}