Investigating the Effects of Ibuprofen on the Gene Expression Profile in Hippocampus of Mice Model of Alzheimer's Disease through Bioinformatics Analysis.

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One of which is neurodegenerative diseases including Alzheimer disease (AD). In this study gross alteration of gene expression in AD mice by ibuprofen treatment is investigated via Protein-protein interaction network (PPI) analysis. Expression profiling of microarray dataset GSE67306 was retrieved from GEO database and analyzed via GEO2R tool. PPI analysis was performed via Cytoscape 3.7.0. and its plug-ins including Network Analyzer, Gene MANIA, and CluePedia. Numbers of 10 central genes including Htr1a, Sstr2, Drd2, Htr1b, Penk, Pomc, Oprm1, Npy, Sst, and Chrm2 were identified as potential biomarkers. However, the role of Penk gene was highlighted. The finding indicates that ibuprofen changes gene expression level of several genes that are involved in AD.", "claims": [{"id": "claim-method", "statement": "Before the t-test, box plotting was used for cross comparison between groups of interest.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33224242", "locator": "sections[2] \"Experimental\" paragraph 4", "quote": "Before the t-test, box plotting was used for cross comparison between groups of interest."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The expression data GEO series accession number= GSE67306 via platform, GPL6246 was obtained from the Gene Expression Omnibus (GEO, http://www.ncbi.nlm.nih.gov/geo/) (13). The conducted study was entitled “Effect of ibuprofen on hippocampal gene expression in APP-PS1 mice”. 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The finding was Public on Jun 14, 2016 in GEO database.", "access_statement_kind": "repository_route_statement", "sample_accessions": ["GSM1644124", "GSM1644125", "GSM1644126", "GSM1644127", "GSM1644128", "GSM1644129", "GSM1644130", "GSM1644131", "GSM1644132", "GSM1644133", "GSM1644134", "GSM1644135", "GSM1644136", "GSM1644137", "GSM1644139", "GSM1644140", "GSM1644141", "GSM1644142", "GSM1644143"], "sample_count": 19, "group_sizes": null, "group_sizes_basis": null, "platform": "6246", "taxon": "Mus musculus", "title": "Effect of ibuprofen on hippocampal gene expression in APP-PS1 mice", "resolution_checked_at": "2026-11-01T15:39:55Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "asset:table:pmid:33224242:1", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:c3cb4c083c9f9fe3b5cfba0d67ac31d4175041b43c71ac27d4e633bbead9e3dd", "bytes": 739, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[3]/table-wrap[1]#T2", "version": null, "note": "The most significant DEGs scores in the treated AD mice with ibuprofen relative to the AD mice. 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Top 10% of genes based on degree, betweenness and closeness were considered and the genes that were common in the two centrality list were identified as central elements. K; degree, BC; Betweenness centrality, and CC; Closeness centrality. The asterisked genes are common between three groups of central nodes", "structure": {"pmcid": "PMC7667553", "section_index": 3, "section_title": "Results", "row_count": 11, "column_count": 6}, "table": {"label": "Table 3", "caption": "The list of central genes with high centrality values. Top 10% of genes based on degree, betweenness and closeness were considered and the genes that were common in the two centrality list were identified as central elements. K; degree, BC; Betweenness centrality, and CC; Closeness centrality. 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