Identification of 10 Hub genes related to the progression of colorectal cancer by co-expression analysis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:33240582", "kind": "science-record", "revision": 7, "content_hash": "sha256:2ffaf1cf398b5aea78eb29f66d6273f19e2f03fb06080ff9c421b97a74021a5f", "created_at": "2026-11-01T15:41:17Z", "updated_at": "2026-11-01T22:02:36Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Identification of 10 Hub genes related to the progression of colorectal cancer by co-expression analysis.", "doi": "10.7717/peerj.9633", "pmid": "33240582", "authors": ["Jie Meng", "Rui Su", "Yun Liao", "Yanyan Li", "Ling Li"], "journal": "PeerJ", "year": 2020, "abstract": "BACKGROUND: Colorectal cancer (CRC) is the third most common cancer in the world. The present study is aimed at identifying hub genes associated with the progression of CRC.\nMETHOD: The data of the patients with CRC were obtained from the Gene Expression Omnibus (GEO) database and assessed by weighted gene co-expression network analysis (WGCNA), Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses performed in R by WGCNA, several hub genes that regulate the mechanism of tumorigenesis in CRC were identified. Differentially expressed genes in the data sets GSE28000 and GSE42284 were used to construct a co-expression network for WGCNA. The yellow, black and blue modules associated with CRC level were filtered. Combining the co-expression network and the PPI network, 15 candidate hub genes were screened.\nRESULTS: After validation using the TCGA-COAD dataset, a total of 10 hub genes (MT1X, MT1G, MT2A, CXCL8, IL1B, CXCL5, CXCL11, IL10RA, GZMB, KIT) closely related to the progression of CRC were identified. The expressions of MT1G, CXCL8, IL1B, CXCL5, CXCL11 and GZMB in CRC tissues were higher than normal tissues (p-value < 0.05). The expressions of MT1X, MT2A, IL10RA and KIT in CRC tissues were lower than normal tissues (p-value < 0.05).\nCONCLUSIONS: By combinating with a series of methods including GO enrichment analysis, KEGG pathway analysis, PPI network analysis and gene co-expression network analysis, we identified 10 hub genes that were associated with the progression of CRC.", "claims": [{"id": "claim-method", "statement": "Kaplan–Meier—Plotter database (http://kmplot.com/analysis/) in CRC data set were used for survival analysis.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33240582", "locator": "sections[9] \"Hub gene survival analysis\" paragraph 2", "quote": "Kaplan–Meier—Plotter database (http://kmplot.com/analysis/) in CRC data set were used for survival analysis."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The following information was supplied regarding data availability:\nThe raw measurements are available in the Supplemental Files.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33240582", "locator": "sections[32] \"Data Availability Statement\" paragraphs 1-3", "quote": "The following information was supplied regarding data availability:\nThe raw measurements are available in the Supplemental Files."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:33240582"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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