FRZB as a key molecule in abdominal aortic aneurysm progression affecting vascular integrity.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:33245093", "kind": "science-record", "revision": 6, "content_hash": "sha256:7509348c650379defdbd4d181ca10010aceb7a3ad1aa153a328c0fb2ae05caea", "created_at": "2026-11-01T15:41:17Z", "updated_at": "2026-11-01T21:24:10Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "FRZB as a key molecule in abdominal aortic aneurysm progression affecting vascular integrity.", "doi": "10.1042/BSR20203204", "pmid": "33245093", "authors": ["Chang-Kyu Oh", "Yeji Ko", "Jeong Jun Park", "Hye Jin Heo", "Junho Kang", "Eun Jung Kwon", "Ji Wan Kang", "Yoonsung Lee", "Kyungjae Myung", "Jin Mo Kang", "Dai Sik Ko", "Yun Hak Kim"], "journal": "Bioscience reports", "year": 2021, "abstract": "Abdominal aortic aneurysm (AAA), when ruptured, results in high mortality. The identification of molecular pathways involved in AAA progression is required to improve AAA prognosis. The aim of the present study was to assess the key genes for the progression of AAA and their functional role. Genomic and clinical data of three independent cohorts were downloaded from the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) (GSE57691, GSE7084, and GSE98278). To develop AAA diagnosis and progression-related differentially expressed genes (DEGs), we used a significance analysis of microarray (SAM). Spearman correlation test and gene set analysis were performed to identify potential enriched pathways for DEGs. Only the Frizzled-related protein (FRZB) gene and chromosome 1 open reading frame 24 (C1orf24) exhibited significant down-regulation in all analyses. With FRZB, the pathways were associated with RHO GTPase and elastin fiber formation. With C1orf24, the pathways were elastic fiber formation, extracellular matrix organization, and cell-cell communication. Since only FRZB was evolutionally conserved in the vertebrates, function of FRZB was validated using zebrafish embryos. Knockdown of frzb remarkably reduced vascular integrity in zebrafish embryos. We believe that FRZB is a key gene involved in AAA initiation and progression affecting vascular integrity.", "claims": [{"id": "claim-method", "statement": "In this study, we performed the SAM tests in the ‘siggenes’ package R (version 3.6.1).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33245093", "locator": "sections[6] \"Significance analysis of microarray\" paragraph 2", "quote": "In this study, we performed the SAM tests in the ‘siggenes’ package R (version 3.6.1)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The data that support the findings of the present study are available from the corresponding author upon reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33245093", "locator": "sections[18] \"Data Availability\" paragraph 2", "quote": "The data that support the findings of the present study are available from the corresponding author upon reasonable request."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:33245093"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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"reader_status": "available", "identifier": "section[13]/table-wrap[1]#T2", "version": null, "note": "Top ten significantly enriched Reactome pathways with FRZB and correlated genes from GSE57691 and GSE7084 using Enrichr (https://amp.pharm.mssm.edu/Enrichr/)", "structure": {"pmcid": "PMC7789806", "section_index": 13, "section_title": "Correlation analysis", "row_count": 11, "column_count": 6}, "table": {"label": "Table 2", "caption": "Top ten significantly enriched Reactome pathways with FRZB and correlated genes from GSE57691 and GSE7084 using Enrichr (https://amp.pharm.mssm.edu/Enrichr/)", "rows": [["Term", "Overlap", "P-value", "Odds ratio", "Combined score", "Genes"], ["RHO GTPases activate ROCKs", "3/17", "2.16E-05", "54.29864253", "583.4084401", "PPP1CB; MYH11; MYH10"], ["RHO GTPases activate PAKs", "3/21", "4.18E-05", "43.95604396", "443.2037946", "PPP1CB; MYH11; MYH10"], ["Molecules associated with elastic fibers", "3/30", "1.25E-04", "30.76923077", "276.5468079", "MFAP4; 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"media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:0ed89f66a2b35e6a222e33d10feb8260e5e43bdc73d053cd06a751549026b2ac", "bytes": 1369, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[13]/table-wrap[2]#T3", "version": null, "note": "Top ten significantly enriched Reactome pathways with C1orf24 and correlated genes from GSE57691 and GSE7084 using Enrichr (https://amp.pharm.mssm.edu/Enrichr/)", "structure": {"pmcid": "PMC7789806", "section_index": 13, "section_title": "Correlation analysis", "row_count": 11, "column_count": 6}, "table": {"label": "Table 3", "caption": "Top ten significantly enriched Reactome pathways with C1orf24 and correlated genes from GSE57691 and GSE7084 using Enrichr (https://amp.pharm.mssm.edu/Enrichr/)", "rows": [["Term", "Overlap", "P-value", "Odds ratio", "Combined score", "Genes"], ["Elastic fiber formation", "3/41", "4.32E-04", "20.32520325", "157.4485645", "MFAP4; 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