A Novel Circulating MiRNA-Based Signature for the Diagnosis and Prognosis Prediction of Early-Stage Cervical Cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:33327867", "kind": "science-record", "revision": 7, "content_hash": "sha256:9943b44cb42000499f51cab2a49d47414637b401e10e827651332aea396dfd55", "created_at": "2026-11-01T15:41:19Z", "updated_at": "2026-11-01T21:24:19Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "A Novel Circulating MiRNA-Based Signature for the Diagnosis and Prognosis Prediction of Early-Stage Cervical Cancer.", "doi": "10.1177/1533033820970667", "pmid": "33327867", "authors": ["Huajuan Qiu", "Duoxian Liang", "Limin Liu", "Qun Xiang", "Zhijun Yi", "Yanqin Ji"], "journal": "Technology in cancer research & treatment", "year": 2020, "abstract": "BACKGROUND: MicroRNAs (miRNAs) have been shown to play a key role in regulating the progression of cervical cancer (CC). This study aimed to develop a circulating miRNA-based molecular signature for the diagnosis and prognosis prediction of early-stage CC.\nMETHODS: This study included 112 patients diagnosed with early-stage CC, 45 patients confirmed with cervical intraepithelial neoplasia (CIN) and 90 healthy subjects. Compared to the normal controls, the expression level of miR-21 was increased, while the levels of miR-125b and miR-370 were decreased in CC in both GSE30656 and The Cancer Genome Atlas (TCGA) cohort. The expression levels and diagnostic value of these candidate miRNAs were then validated through qRT-PCR. Their diagnostic and prognostic values for early-stage CC were further explored.\nRESULTS: Compared to the patients with CIN and healthy subjects, serum miR-21 was increased, while serum miR-125b and serum miR-370 were reduced in early-stage CC. In addition, combining these molecules yielded good performance for differentiating early-stage CC from CIN or healthy subjects. Moreover, strong association was found between serum miR-21 and lymph node metastasis (LNM) as well as recurrence-free survival of early-stage CC. Similar observations were found for serum miR-125b and serum miR-370. Patients with simultaneously high serum miR-21 + low serum miR-125b + low serum miR-370 suffered a high risk of LNM and recurrence, while those with low serum miR-21 + high serum miR-125b + high serum miR-370 had little risk for LNM and recurrence.\nCONCLUSIONS: Combining serum miR-21, miR-125b and miR-370 as a miRNA-based signature is promising for the detection and prognosis prediction of early-stage CC.", "claims": [{"id": "claim-method", "statement": "As the data was not normally distributed, the nonparametric Kruskal-Wallis test was used to compare the differences of circulating miRNAs among different groups.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33327867", "locator": "sections[6] \"Statistical Analysis\" paragraph 2", "quote": "As the data was not normally distributed, the nonparametric Kruskal-Wallis test was used to compare the differences of circulating miRNAs among different groups."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Two studies namely GSE30656 and TCGA cohort were included. Then the commonly and significantly changed miRNAs between cervical cancer and normal cervical epithelium were screened and identified. The cut-off was set as fold change≥2 and P < 0.05. Compared to the normal controls, the expression level of miR-21 was increased, while the levels of miR-125b and miR-370 were decreased in CC in both GSE30656 and TCGA cohort. Therefore, these candidate miRNAs were selected for further validation. For the number of included patients, there were 10 CC cases and 10 controls in GSE30656, as well as 299 CC cases and 3 controls in the TCGA cohort.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33327867", "locator": "sections[8] \"The Diagnostic Values of Serum miR-21, miR-125b and miR-370 in Early-Stage CC\" paragraph 2", "quote": "Two studies namely GSE30656 and TCGA cohort were included. Then the commonly and significantly changed miRNAs between cervical cancer and normal cervical epithelium were screened and identified. The cut-off was set as fold change≥2 and P < 0.05. Compared to the normal controls, the expression level of miR-21 was increased, while the levels of miR-125b and miR-370 were decreased in CC in both GSE30656 and TCGA cohort. Therefore, these candidate miRNAs were selected for further validation. 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Then the commonly and significantly changed miRNAs between cervical cancer and normal cervical epithelium were screened and identified. The cut-off was set as fold change≥2 and P < 0.05. Compared to the normal controls, the expression level of miR-21 was increased, while the levels of miR-125b and miR-370 were decreased in CC in both GSE30656 and TCGA cohort. Therefore, these candidate miRNAs were selected for further validation. For the number of included patients, there were 10 CC cases and 10 controls in GSE30656, as well as 299 CC cases and 3 controls in the TCGA cohort.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "Two studies namely GSE30656 and TCGA cohort were included. Then the commonly and significantly changed miRNAs between cervical cancer and normal cervical epithelium were screened and identified. The cut-off was set as fold change≥2 and P < 0.05. Compared to the normal controls, the expression level of miR-21 was increased, while the levels of miR-125b and miR-370 were decreased in CC in both GSE30656 and TCGA cohort. Therefore, these candidate miRNAs were selected for further validation. 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