GFOGER Peptide Modifies the Protein Content of Extracellular Vesicles and Inhibits Vascular Calcification.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:33330469", "kind": "science-record", "revision": 7, "content_hash": "sha256:efc28eaf4252fdbf57910dab05eda820fce4c4fb3403f2adc317545de3aafd56", "created_at": "2026-11-01T15:41:19Z", "updated_at": "2026-11-01T22:02:46Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "GFOGER Peptide Modifies the Protein Content of Extracellular Vesicles and Inhibits Vascular Calcification.", "doi": "10.3389/fcell.2020.589761", "pmid": "33330469", "authors": ["Ali Mansour", "Walaa Darwiche", "Linda Yaker", "Sophie Da Nascimento", "Cathy Gomila", "Claire Rossi", "Vincent Jung", "Pascal Sonnet", "Saïd Kamel", "Ida Chiara Guerrera", "Agnès Boullier", "Jérôme Ausseil"], "journal": "Frontiers in cell and developmental biology", "year": 2020, "abstract": "OBJECTIVE: Vascular calcification (VC) is an active process during which vascular smooth muscle cells (VSMCs) undergo an osteogenic switch and release extracellular vesicles (EVs). In turn, the EVs serve as calcification foci via interaction with type 1 collagen (COL1). We recently showed that a specific, six-amino-acid repeat (GFOGER) in the sequence of COL1 was involved in the latter's interaction with integrins expressed on EVs. Our main objective was to test the GFOGER ability to inhibit VC.\nAPPROACH: We synthesized the GFOGER peptide and tested its ability to inhibit the inorganic phosphate (Pi)-induced calcification of VSMCs and aortic rings. Using mass spectrometry, we studied GFOGER's effect on the protein composition of EVs released from Pi-treated VSMCs.\nRESULTS: Calcification of mouse VSMCs (MOVAS-1 cells), primary human VSMCs, and rat aortic rings was lower in the presence of GFOGER than with Pi alone (with relative decreases of 66, 58, and 91%, respectively; p < 0.001 for all) (no effect was observed with the scramble peptide GOERFG). A comparative proteomic analysis of EVs released from MOVAS-1 cells in the presence or absence of Pi highlighted significant differences in EVs' protein content. Interestingly, the expression of some of the EVs' proteins involved in the calcification process (such as osteogenic markers, TANK-binding kinase 1, and casein kinase II) was diminished in the presence of GFOGER peptide (data are available via ProteomeXchange with identifier PXD018169∗). The decrease of osteogenic marker expression observed in the presence of GFOGER was confirmed by q-RT-PCR analysis.\nCONCLUSION: GFOGER peptide reduces vascular calcification by modifying the protein content of the subsequently released EVs, in particular by decreasing osteogenicswitching in VSMCs.", "claims": [{"id": "claim-method", "statement": "A t-test was used to compare treatment groups with the control (FDR: 0.05; S0: 0.1), and the data are represented as volcano plots.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33330469", "locator": "sections[19] \"Data Processing After the LC-MS/MS Acquisition\" paragraph 3", "quote": "A t-test was used to compare treatment groups with the control (FDR: 0.05; S0: 0.1), and the data are represented as volcano plots."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Our proteomic dataset has been uploaded to Proteome Xchange repository—accession PXD018169.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33330469", "locator": "sections[30] \"Data Availability Statement\" paragraph 2", "quote": "Our proteomic dataset has been uploaded to Proteome Xchange repository—accession PXD018169."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:33330469"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC7734313", "source": "europe_pmc_fulltext", "coverage": "whole_body_without_references", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:PXD018169", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=PXD018169", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "PXD018169", "version": null, "note": "Our proteomic dataset has been uploaded to Proteome Xchange repository—accession PXD018169.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "Our proteomic dataset has been uploaded to Proteome Xchange repository—accession PXD018169.", "access_statement_kind": "data_availability_statement", "sample_accessions": [], "sample_count": null, "group_sizes": null, "group_sizes_basis": null, "platform": null, "taxon": null, "title": "Extracellular Vesicles proteind in vascular calcification", "resolution_checked_at": "2026-10-31T18:35:55Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "asset:table:pmid:33330469:1", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:bf3bfe27c3a2862a5ec715e41d10ef4a79d7ba3fcf9f77b043f9e49328692e9b", "bytes": 868, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[20]/table-wrap[1]#T1", "version": null, "note": "Forward and reverse primers used to quantify transcription of the genes of interest.", "structure": {"pmcid": "PMC7734313", "section_index": 20, "section_title": "RNA Extraction and Quantitative Real-Time PCR", "row_count": 13, "column_count": 3}, "table": {"label": "TABLE 1", "caption": "Forward and reverse primers used to quantify transcription of the genes of interest.", "rows": [["Gene", "Primer", "Sequence"], ["ARP0", "Forward", "5′-TCCAGAGGCACCATTGAAATT-3′"], ["", "Reverse", "5′-TCGCTGGCTCCCACCTT-3’"], ["Runx2", "Forward", "5′-AGGCACAGACAGAAGCTTGATG-3′"], ["", "Reverse", "5′-GCGATCAGAGAACAAACTAGGTT-3′"], ["OCN", "Forward", "5′-GACCGCCTACAAACGCATCT-3′"], ["", "Reverse", "5′-GGGCAGCACAGGTCCTEEATAGT-3′"], ["MGP", "Forward", "5′-ATGAAGAGCCTGCTCCCTCT-3′"], ["", "Reverse", "5′-ATATTTGGCTCCTCGGCGCT-3′"], ["TNAP", "Forward", "5′-CTGCCACTGCCTACTTGTGT-3′"], ["", "Reverse", "5′-GATGGATGTGACCTCATTGC-3′"], ["SMPD3", "Forward", "5′-AGAAACCCGGTCCTCGTACT-3′"], ["", "Reverse", "5′-CCTGACCAGTGCCATTCTTT-3′"]], "row_count": 13, "column_count": 3, "numeric_cells": 12, "header_rows": 1, "locator": "section[20]/table-wrap[1]#T1"}}], "gaps": ["asset:data:geo:PXD018169 not downloaded", "asset:data:geo:PXD018169 licence not read"]}, "analysis": {"state": "partial", "methods": [{"id": "method-1", "family": "group_comparison", "specific": "anova", "label": "Group comparison", "dsl_operations": ["group_summary", "welch_t", "bootstrap_mean_difference", "group_comparison_anova"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["two_or_more_groups_continuous"], "evidence": {"score": 0.8, "band": "high", "quote": "ANOVA"}}, {"id": "method-2", "family": null, "specific": null, "label": "t-test", "dsl_operations": [], "missing_operations": [], "expressible": null, "parameters": {}, "data_shapes": [], "evidence": {"score": 1.0, "band": "verbatim", "quote": "A t-test was used to compare treatment groups with the control (FDR: 0.05; 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Using mass spectrometry, we studied GFOGER's effect on the protein c", "ewed twice a week. Data Processing After the LC-MS/MS Acquisition Raw MS data files were processed", "the scramble peptide GOERFG). A comparative proteomic analysis of EVs released from MOVAS-1 cells", "represented as volcano plots. Lastly, the MS proteomics data were deposited with the ProteomeXchange"], "order_basis": "paper_text_order:725", "id": "assay:1"}, {"role": "data_generation", "definition_id": "animal_model", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["Pi-treated VSMCs. RESULTS: Calcification of mouse VSMCs (MOVAS-1 cells), primary human VSMCs,", "Cs (MOVAS-1 cells), primary human VSMCs, and rat aortic rings was lower in the presence of GF", "igma–Aldrich (St. Louis, MO, United States). Murine Vascular Smooth Muscle Cell Culture Murine a", ". Murine Vascular Smooth Muscle Cell Culture Murine aortic VSMCs (MOVAS-1 CRL-2797, ATCC, Manass"], "order_basis": "paper_text_order:864", "id": "assay:2"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["l pieces (1–2 mm2) of the medial tissue were cultured in Petri dishes containing DMEM 6546 with 15% F", "ant. At confluence, cells were harvested and cultured in T75 flasks. The VSMCs were then cultured in", "cultured in T75 flasks. 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