Identifying the p65-Dependent Effect of Sulforaphene on Esophageal Squamous Cell Carcinoma Progression via Bioinformatics Analysis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:33374641", "kind": "science-record", "revision": 3, "content_hash": "sha256:fa0669f90fa7a6cd0720e7eff4448c7e616583c6cb6df37162f426f327d6fa5c", "created_at": "2026-11-01T15:41:20Z", "updated_at": "2026-11-01T21:24:23Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Identifying the p65-Dependent Effect of Sulforaphene on Esophageal Squamous Cell Carcinoma Progression via Bioinformatics Analysis.", "doi": "10.3390/ijms22010060", "pmid": "33374641", "authors": ["Sichong Han", "Zhe Wang", "Jining Liu", "Qipeng Yuan"], "journal": "International journal of molecular sciences", "year": 2020, "abstract": "Understanding the mechanism by which sulforaphene (SFE) affects esophageal squamous cell carcinoma (ESCC) contributes to the application of this isothiocyanate as a chemotherapeutic agent. Thus, we attempted to investigate SFE regulation of ESCC characteristics more deeply. We performed gene set enrichment analysis (GSEA) on microarray data of SFE-treated ESCC cells and found that differentially expressed genes are enriched in TNFα_Signaling_via_the_NFκB_Pathway. Coupled with the expression profile data from the GSE20347 and GSE75241 datasets, we narrowed the set to 8 genes, 4 of which (C-X-C motif chemokine ligand 10 (CXCL10), TNF alpha induced protein 3 (TNFAIP3), inhibin subunit beta A (INHBA), and plasminogen activator, urokinase (PLAU)) were verified as the targets of SFE. RNA-sequence (RNA-seq) data of 182 ESCC samples from The Cancer Genome Atlas (TCGA) were grouped into two phenotypes for GSEA according to the expression of CXCL10, TNFAIP3, INHBA, and PLAU. The enrichment results proved that they were all involved in the NFκB pathway. ChIP-seq analyses obtained from the Cistrome database indicated that NFκB-p65 is likely to control the transcription of CXCL10, TNFAIP3, INHBA, and PLAU, and considering TNFAIP3 and PLAU are the most significantly differentially expressed genes, we used chromatin immunoprecipitation-polymerase chain reaction (ChIP-PCR) to verify the regulation of p65 on their expression. The results demonstrated that SFE suppresses ESCC progression by down-regulating TNFAIP3 and PLAU expression in a p65-dependent manner.", "claims": [{"id": "claim-method", "statement": "GSEA v2.0 [34] was used to determine enrichment of significantly DEGs in microarray data of EC109 and KYSE510 cells treated with 20 μM SFE, and the high mRNA levels of CXCL10, TNFAIP3, INHBA, and PLAU-related GO annotations and pathways in Illumina HiSeq 2000 RNA Sequencing of TCGA ESCC data.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33374641", "locator": "sections[19] \"4.11. 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