Overexpression of a Long Non-Coding RNA BC037916 is Associated with Pancreatic Tumorigenesis and Poor Prognosis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:33447050", "kind": "science-record", "revision": 7, "content_hash": "sha256:d9aab8a38c550fbb392648284b7cc5eddd6fd15e765ae0295ab5a4b6242442d2", "created_at": "2026-11-01T15:41:22Z", "updated_at": "2026-11-01T22:45:58Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Overexpression of a Long Non-Coding RNA BC037916 is Associated with Pancreatic Tumorigenesis and Poor Prognosis.", "doi": "10.2147/OTT.S282350", "pmid": "33447050", "authors": ["Gang Chen", "Litao Xu", "Guanxiong Ye", "Junhua Lin", "Zhiqiang Meng", "Yehua Shen"], "journal": "OncoTargets and therapy", "year": 2020, "abstract": "BACKGROUND: Pancreatic cancer is one of the most lethal malignancies. Accumulating evidence supports for the critical contribution of long noncoding RNAs (lncRNAs) to the cancer development and progression. We tried to identify novel lncRNAs involved in the pancreatic carcinogenesis.\nMATERIALS AND METHODS: Two independent datasets (Gene Expression Omnibus datasets: GSE16515 and GSE32688) were obtained from the Gene Expression Omnibus (GEO). The level of BC037916 was detected in pancreatic cancer tissues and adjacent no-tumorous tissues (n=86) by qRT-PCR. Effects of BC037916 on proliferation, apoptosis, and invasion of pancreatic cancer cells were examined.\nRESULTS: We identified a novel lncRNA BC037916 involved in the pancreatic carcinogenesis by analyzing GEO datasets. Quantitative RT-PCR analysis showed that 86.0% (74/86) pancreatic cancer tissues had increased BC037916 expression as compared with normal counterparts. Further, positive correlation was observed between BC037916 expression and clinical stage, primary tumor, and regional lymph node invasion. Importantly, BC037916 was an independent prognostic factor of pancreatic cancer. Functionally, knockdown of BC037916 repressed cell proliferation, inhibited cell invasion, halted cell cycle progression, and promoted apoptosis in both PANC-1 and SW1990 cells. In contrast, overexpression of BC037916 in CAPAN-1 had opposite effects. Moreover, silencing of BC037916 significantly inhibited the tumor growth of xenografted SW1990 cells in vivo. Results of Western blot assays suggested that BC037916 knockdown also suppressed the activation of JAK2/STAT3 and TGF-β signaling. Further experiments suggested that BC037916 positively regulated the expression of Twist through miR-3145-3p.\nCONCLUSION: BC037916 exhibited oncogenic potential in pancreatic cancer development.", "claims": [{"id": "claim-method", "statement": "Data were analyzed by SPSS software (v16.0, SPSS, Inc., Chicago, IL, USA) and shown as mean ± standard deviation (SD). Student’s t-test was carried out to compare the differences between the two groups. The clinical characteristics of tumors with differential BC037916 expression were determined by the Chi-square tests. Cox regression model was employed to determine univariate and multivariate hazard ratios. Patients’ survival analyses were carried out using Kaplan-Meier analysis and Log-rank test.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33447050", "locator": "sections[15] \"Statistical Analysis\" paragraph 2", "quote": "Data were analyzed by SPSS software (v16.0, SPSS, Inc., Chicago, IL, USA) and shown as mean ± standard deviation (SD). Student’s t-test was carried out to compare the differences between the two groups. The clinical characteristics of tumors with differential BC037916 expression were determined by the Chi-square tests. Cox regression model was employed to determine univariate and multivariate hazard ratios. Patients’ survival analyses were carried out using Kaplan-Meier analysis and Log-rank test."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33447050", "locator": "sections[32] \"Data Sharing Statement\" paragraph 2", "quote": "The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:33447050"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE16515"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE16515"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:33447050", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.2147/OTT.S282350", "sha256": 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[["Clinicopathologic features", "Total", "BC037916 Expression", "P value"], ["", "", "Low (n=43)", "High (n=43)", ""], ["Age (years)", "", "", "", ""], ["≤65", "56", "32", "24", "0.1125"], [">65", "30", "11", "19", ""], ["Gender", "", "", "", ""], ["Male", "50", "22", "28", "0.2744"], ["Female", "36", "21", "15", ""], ["Tumor size", "", "", "", ""], [">4cm", "35", "18", "17", "1.000"], ["≤4cm", "51", "25", "26", ""], ["Tumor location", "", "", "", ""], ["Head", "49", "25", "24", "1.000"], ["Body/tail", "37", "18", "17", ""], ["Clinical stage (AJCC)", "", "", "", ""], ["I–II", "66", "38", "28", "0.0202*"], ["III–IV", "20", "5", "15", ""], ["T-Primary tumor", "", "", "", ""], ["T1-T2", "48", "30", "18", "0.0164*"], ["T3-T4", "38", "13", "25", ""], ["N-Regional lymph nodes", "", "", "", ""], ["N0", "51", "31", "20", "0.0275*"], ["N1", "35", "12", "23", ""], ["M-Distant metastasis", "", "", "", ""], ["M0", "77", "40", "37", "0.4833"], ["M1", "9", "3", "6", ""]], "row_count": 26, "column_count": 5, "numeric_cells": 63, "header_rows": 2, "locator": "section[19]/table-wrap[1]#t0001"}}, {"id": "asset:table:pmid:33447050:2", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:9f4f9a54f911eb681f69bf065d055b8014e64a1d0a4cf7a447e277bbe515ed79", "bytes": 1074, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[20]/table-wrap[1]#t0002", "version": null, "note": "Cox Regression Analysis of BC037916 Expression with Cancer-Specific Mortality in Pancreatic Cancer", "structure": {"pmcid": "PMC7801918", "section_index": 20, "section_title": "BC037916 Serves as an Independent Prognostic Factor for Pancreatic Cancer", "row_count": 11, "column_count": 5}, "table": {"label": "Table 2", "caption": "Cox Regression Analysis of BC037916 Expression with Cancer-Specific Mortality in Pancreatic Cancer", "rows": [["", "Univariate Analysis", "Multivariate Analysis"], ["", "HR (95% CI)", "P value", "HR (95% CI)", "P value"], ["BC037916 (high vs low)", "2.81(1.65–4.79)", "0.000***", "2.13 (1.18–3.83", "0.012*"], ["Gender (male vs female)", "0.67 (0.39–1.13)", "0.133", "", ""], ["Age (>65 vs ≤65)", "0.78 (0.46–1.32)", "0.358", "", ""], ["Tumor Size (>4cm vs ≤4cm)", "1.20 (0.71–2.03)", "0.490", "", ""], ["Tumor location (Head vs bodyandtail)", "1.26 (0.76–2.10)", "0.377", "", ""], ["Clinical Stage (I–II vs III–IV)", "2.15 (1.22–3.79)", "0.008**", "1.92 (1.08–3.41)", "0.026*"], ["T-Primary tumor (T1–2 vs T3–4)", "1.76 (1.01–2.93)", "0.030*", "1.32 (0.77–2.27)", "0.315"], ["N-Regional lymph nodes (N0 vs N1)", "2.17 (1.30–3.63)", "0.003**", "1.78 (1.05–3.04)", "0.034*"], ["M-Distant metastasis (M0 vs M1)", "0.88 (0.38–2.04)", "0.762", "", ""]], "row_count": 11, "column_count": 5, "numeric_cells": 32, "header_rows": 2, "locator": "section[20]/table-wrap[1]#t0002"}}], "gaps": ["asset:data:geo:GSE16515 not downloaded", "asset:data:geo:GSE16515 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Student’s t-test was carried out to compare the differences between the two groups. The clinical characteristics of tumors with differential BC037916 expression were determined by the Chi-square tests. Cox regression model was employed to determine univariate and multivariate hazard ratios. 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