Identification and Validation of Autophagy-Related Genes in Chronic Obstructive Pulmonary Disease.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:33469280", "kind": "science-record", "revision": 8, "content_hash": "sha256:5861312be8aeb055f91a003359e08dccdddb0c9356aa4c38dafa3e6f7a30a24a", "created_at": "2026-11-01T15:41:23Z", "updated_at": "2026-11-01T22:46:00Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Identification and Validation of Autophagy-Related Genes in Chronic Obstructive Pulmonary Disease.", "doi": "10.2147/COPD.S288428", "pmid": "33469280", "authors": ["Shulei Sun", "Yuehao Shen", "Jie Wang", "Jinna Li", "Jie Cao", "Jing Zhang"], "journal": "International journal of chronic obstructive pulmonary disease", "year": 2021, "abstract": "PURPOSE: Autophagy plays essential roles in the development of COPD. We aim to identify and validate the potential autophagy-related genes of COPD through bioinformatics analysis and experiment validation.\nMETHODS: The mRNA expression profile dataset GSE38974 was obtained from GEO database. The potential differentially expressed autophagy-related genes of COPD were screened by R software. Then, protein-protein interactions (PPI), correlation analysis, gene-ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were applied for the differentially expressed autophagy-related genes. Finally, RNA expression of top five differentially expressed autophagy-related genes was validated in blood samples from COPD patients and healthy controls by qRT-PCR.\nRESULTS: A total of 40 differentially expressed autophagy-related genes (14 up-regulated genes and 26 down-regulated genes) were identified between 23 COPD patients and 9 healthy controls. The PPI results demonstrated that these autophagy-related genes interacted with each other. The GO and KEGG enrichment analysis of differentially expressed autophagy-related genes indicated several enriched terms related to autophagy and mitophagy. The results of qRT-PCR showed that the expression levels of HIF1A, CDKN1A, BAG3, ERBB2 and ATG16L1 in COPD patients and healthy controls were consistent with the bioinformatics analysis results from mRNA microarray.\nCONCLUSION: We identified 40 potential autophagy-related genes of COPD through bioinformatics analysis. HIF1A, CDKN1A, BAG3, ERBB2 and ATG16L1 may affect the development of COPD by regulating autophagy. 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