High expression of collagen 1A2 promotes the proliferation and metastasis of esophageal cancer cells.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:33490184", "kind": "science-record", "revision": 9, "content_hash": "sha256:259ce5884d4e2947ab80f1f6231273115ed445ce0281ed483c115b13e363584a", "created_at": "2026-11-01T15:41:23Z", "updated_at": "2026-11-01T22:46:01Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "High expression of collagen 1A2 promotes the proliferation and metastasis of esophageal cancer cells.", "doi": "10.21037/atm-20-7867", "pmid": "33490184", "authors": ["Guangbin Li", "Wei Jiang", "Yunteng Kang", "Xiaojun Yu", "Chengpeng Zhang", "Yu Feng"], "journal": "Annals of translational medicine", "year": 2020, "abstract": "BACKGROUND: To undertake a bioinformatics analysis to identify abnormally expressed genes [also referred to as differentially expressed genes (DEGs)] and their functions in esophageal carcinoma (ESCA).\nMETHODS: DEGs (i.e., GSE100942, GSE17351, GSE26886, and GSE77861) were obtained from a gene expression omnibus database. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed using online tools from the Database for Annotation, Visualization and Integrated Discovery. A protein-protein interaction network was then constructed based on the Search Tool for the Retrieval of Interacting Genes website. Cytoscape software was used to identify the top 20 DEGs located in the central region of the network. For the overall survival analysis, a Kaplan-Meier analysis was conducted of the Gene Expression Profiling Interactive Analysis website, and collagen (COL) 1A2 was selected to detect the molecular mechanism of COL1A2-small interfering ribonucleic acid (siRNA) in the following ESCA cell lines: Eca109 and TE-1. Next, the expression of COL1A2-messanger ribonucleic acid was determined using real-time quantitative polymerase chain reaction. The expression of COL1A2 was also verified by Western blot. Cell proliferation was measured by colony-forming and MTT assays, and migration and invasion by the transwell assay.\nRESULTS: Based on the GEO database and screening out the hub gene, we identified that COL1A2 was abnormally expressed in ESCA. With a series of in vitro experiments, the expression of COL1A2 was defined as higher in Eca109 and TE-1.\nCONCLUSIONS: COL1A2 was highly expressed in ESCA tissue samples. Additionally, the proliferation and metastasis of Eca109 and TE-1 cell lines were significantly attenuated by siRNA-COL1A2-mediated small interference. Notably, the expression level of COL1A2 was obviously related to the Akt and epithelial-mesenchymal transition (EMT) pathways.", "claims": [{"id": "claim-method", "statement": "A total of 414 differentially expressed genes (DEGs) were identified in the ESCA tissue samples, and compared with normal esophageal tissue samples using the limma package from the R programming language (15).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33490184", "locator": "sections[3] \"Collection of microarray data\" paragraph 2", "quote": "A total of 414 differentially expressed genes (DEGs) were identified in the ESCA tissue samples, and compared with normal esophageal tissue samples using the limma package from the R programming language (15)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The expression profiles of GSE100942, GSE17351, GSE26886, and GSE77861 in 35 normal esophageal tissue samples and 25 ESCA tissue samples were extracted from the Gene Expression Omnibus (GEO) database of the National Center for Biotechnology Information (https://www.ncbi.nlm.nih.gov/geo/) based on the GPL570 platform.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33490184", "locator": "sections[3] \"Collection of microarray data\" paragraph 2", "quote": "The expression profiles of GSE100942, GSE17351, GSE26886, and GSE77861 in 35 normal esophageal tissue samples and 25 ESCA tissue samples were extracted from the Gene Expression Omnibus (GEO) database of the National Center for Biotechnology Information (https://www.ncbi.nlm.nih.gov/geo/) based on the GPL570 platform."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:33490184"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE100942"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE100942"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:33490184", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.21037/atm-20-7867", "sha256": 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