High expression of MAPK-14 promoting the death of chondrocytes is an important signal of osteoarthritis process.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:33520453", "kind": "science-record", "revision": 5, "content_hash": "sha256:af46696f9f003caaea793b9dece3dd7d3ac152072b07f7969746a97a2131849b", "created_at": "2026-11-01T15:41:23Z", "updated_at": "2026-11-01T18:54:23Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "High expression of MAPK-14 promoting the death of chondrocytes is an important signal of osteoarthritis process.", "doi": "10.7717/peerj.10656", "pmid": "33520453", "authors": ["Zhiqin Deng", "Xiaotian Hu", "Murad Alahdal", "Jianquan Liu", "Zhe Zhao", "Xiaoqiang Chen", "Junxiong Xie", "Li Duan", "Daping Wang", "Wencui Li"], "journal": "PeerJ", "year": 2021, "abstract": "BACKGROUND: Osteoarthritis (OA) is one of the most common degenerative diseases worldwide. Many researchers are studying the pathogenesis of OA, however, it is still unclear.\nMETHODS: Screening and validation of OA relevant hub genes are an important part of exploring their potential molecular mechanism. Therefore, this study aims to explore and verify the mechanisms of hub genes in the OA by bioinformatics, qPCR, fluorescence and propidium iodide staining.\nRESULTS: Microarray datasets GSE43923, GSE55457 and GSE12021 were collected in the Gene Expression Omnibus (GEO), including 45 samples, which divided into 23 osteoarthritis knee joint samples and 22 samples of normal knee joint. Thereafter, 265 differentiallyexpressedgenes (DEGs) were identified in all, which divided into 199 upregulated genes and 66 downregulated genes. The hub genes MAPK-14, PTPRC, PTPN12 were upregulated, while B9D1 was downregulated. In order to further confirm the expression of screening differential genes in human chondrocytes, the human chondrocytes were extracted from a joint replacement surgery and stained with toluidine blue for identification. Compared with normal chondrocytes, OA chondrocytes had high expression of COL I protein and low expression of COL II protein. The expression levels of MAPK-14, PTPRC and PTPN12 in OA chondrocytes were significantly higher than the expression levels of B9D1 in normal chondrocytes. Moreover, the inflammatory necrosis of OA chondrocytes was increased compared with the normal chondrocytes by propidium iodide staining.\nCONCLUSIONS: The high expression of MAPK-14 works as a promoter of chondrocytes death and an important signal of the osteoarthritis process.", "claims": [{"id": "claim-data-access", "statement": "Raw data are available in the Supplemental Files.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33520453", "locator": "sections[26] \"Additional Information and Declarations\" paragraph 5", "quote": "Raw data are available in the Supplemental Files."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:33520453"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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