Bioinformatics Analysis of Key micro-RNAs and mRNAs under the Hand, Foot, and Mouth Disease Virus Infection.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:33574876", "kind": "science-record", "revision": 4, "content_hash": "sha256:7bd026762b327263fbdb922abe0609eca6d633363cebb6b53a67ac99da4745fd", "created_at": "2026-11-01T17:34:43Z", "updated_at": "2026-11-01T22:03:06Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Bioinformatics Analysis of Key micro-RNAs and mRNAs under the Hand, Foot, and Mouth Disease Virus Infection.", "doi": "10.33073/pjm-2020-052", "pmid": "33574876", "authors": ["Sheng Lin", "Liu Yang", "Shibiao Wang", "Bin Weng", "Min Lin"], "journal": "Polish journal of microbiology", "year": 2020, "abstract": "To clarify crucial key micro-RNAs and mRNAs associated with hand, foot, and mouth disease (HFMD) virus infection, we conducted this bioinformatics analysis from four GEO datasets. The following datasets were used for the analysis: GSE85829, GSE94551, GSE52780, and GSE45589. Differentially expressed genes (DEGs) were acquired, and the analysis of functional and pathway enrichment and the relative regulatory network were conducted. After screening common differentially expressed miRNAs (DE-miRNAs), five key miRNAs were acquired: miR-100-3p, miR-125a-3p, miR-1273g-3p, miR-5585-3p, and miR-671-5p. There were three common enriched GO terms between miRNA-derived prediction and mRNA-derived analysis: biosynthetic process, cytosol, and nucleoplasm. There was one common KEGG pathway, i.e., cell cycle shared between miRNA-based and mRNA-based enrichment. Using TarBase V8 in DIANA tools, we acquired 1,520 potential targets (mRNA) from the five key DE-miRNAs, among which the159 DE-mRNAs also included 11 DEGs. These common DEGs showed a PPI network mainly connected by SMC1A, SMARCC1, SF3B3, LIG1, and BRMS1L. Together, changes in five key miRNAs and 11 key mRNAs may play crucial roles in HFMD progression. A combination of these roles may benefit the early diagnosis and treatment of HFMD.", "claims": [{"id": "claim-method", "statement": "For dataset GSE45589 (mRNA expression), there were two independent samples. Each sample presented the degree of FC after SH-SY5Y cells were infected with EV71 and those genes with FC ratios ≥ 2 or ≤ 0.5 and p < 0.05 were selected as DEGs.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33574876", "locator": "sections[3] \"Materials and Methods\" paragraph 3", "quote": "For dataset GSE45589 (mRNA expression), there were two independent samples. 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