Quantitative proteomics analysis of Mycoplasma pneumoniae identifies potential macrolide resistance determinants.

{"sci_version": "1.0", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:33580372", "kind": "science-record", "revision": 4, "content_hash": "sha256:5e9250b63d8e5909bc677984c846f9c53b4b534509b8d9ee226b5187a66471a2", "created_at": "2026-11-01T15:41:25Z", "updated_at": "2026-11-01T21:24:40Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Quantitative proteomics analysis of Mycoplasma pneumoniae identifies potential macrolide resistance determinants.", "doi": "10.1186/s13568-021-01187-8", "pmid": "33580372", "authors": ["Shaoli Li", "Guanhua Xue", "Hanqing Zhao", "Yanling Feng", "Chao Yan", "Jinghua Cui", "Xianghui Xie", "Jing Yuan"], "journal": "AMB Express", "year": 2021, "abstract": "Mycoplasma pneumoniae is one of the leading causes of community-acquired pneumonia in children and adolescents. Because of the wide application of macrolides in clinical treatment, macrolide-resistant M. pneumoniae strains have become increasingly common worldwide. However, the molecular mechanisms underlying drug resistance in M. pneumoniae are poorly understood. In the present work, we analyzed the whole proteomes of macrolide-sensitive and macrolide-resistant strains of M. pneumoniae using a tandem mass tag-labeling quantitative proteomic technique, Data are available via ProteomeXchange with identifier PXD022220. In total, 165 differentially expressed proteins were identified, of which 80 were upregulated and 85 were downregulated in the drug-resistant strain compared with the sensitive strain. Functional analysis revealed that these proteins were predominantly involved in protein and peptide biosynthesis processes, the ribosome, and transmembrane transporter activity, which implicates them in the mechanism(s) of resistance of M. pneumoniae to macrolides. Our results provide new insights into drug resistance in M. pneumoniae and identify potential targets for further studies on resistance mechanisms in this bacterium.", "claims": [{"id": "claim-method", "statement": "Differences in the expression levels of six selected DEPs in the PRM analysis between the macrolide-resistant M. pneumoniae strain C267 and the macrolide-sensitive M. pneumoniae strain M129 were determined using a t-test, and p < 0.10 was taken to indicate statistical significance.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33580372", "locator": "sections[11] \"Statistical analysis\" paragraph 2", "quote": "Differences in the expression levels of six selected DEPs in the PRM analysis between the macrolide-resistant M. pneumoniae strain C267 and the macrolide-sensitive M. pneumoniae strain M129 were determined using a t-test, and p < 0.10 was taken to indicate statistical significance."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33580372", "locator": "sections[23] \"notes\" paragraph 1", "quote": "The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:33580372"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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